AB96882

Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed

Name: Goat Anti-Mouse IgG (DyLight® 650) preadsorbed secondary antibody (ab96882) | Abcam
Brand: Abcam Limited
SKU: AB96882
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 650 replaces DyLight® 649.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:2201-2216 PubMed41044342

2025

Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Ionescu,Lior Ankol,Anand Ganapathy Subramaniam,Topaz Altman,Iddo Magen,Yahel Cohen,Yehuda Danino,Tal Gradus-Pery,Yoav Niv,Ori Bar Avi,Danielle Geller,Amjd Ibraheem,Ruilei Cheng,Noam Steinberg,Leenor Alfahel,Pauline Duc,Zeynep Ergul-Ulger,Doruk Arslan,Ersin Tan,Florence Rage,Nilo Riva,Angello Quattrini,Can Ebru Bekircan-Kurt,Adrian Israelson,Amir Dori,Eran Hornstein,Eran Perlson

Scientific reports 15:9206 PubMed40097560

2025

ZIP13 marks muscle satellite cells and contributes to their quiescent and active phase balance.

Applications

Unspecified application

Species

Unspecified reactive species

Emi Yoshigai,Takafumi Hara,Masaki Hashimoto,Hidenao Tsuzuki,Takaya Abe,Kenichi Inoue,Ayaka Noguchi,Takuto Ohashi,Toshiyuki Fukada

ACS pharmacology & translational science 6:320-333 PubMed36798475

2023

Development of Brain-Derived Bioscaffolds for Neural Progenitor Cell Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Julia C Terek,Matthew O Hebb,Lauren E Flynn

Scientific reports 12:22641 PubMed36587177

2022

Live probiotic bacteria administered in a pathomimetic Leaky Gut Chip ameliorate impaired epithelial barrier and mucosal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Soyoun Min,Nam Than,Yong Cheol Shin,Grace Hu,Woojung Shin,Yoko M Ambrosini,Hyun Jung Kim

Cellular and molecular gastroenterology and hepatology 13:289-307 PubMed34536564

2021

C-Reactive Protein Protects Against Acetaminophen-Induced Liver Injury by Preventing Complement Overactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Yun Li,Zhao-Ming Tang,Zhe Wang,Jian-Min Lv,Xiao-Ling Liu,Yu-Lin Liang,Bin Cheng,Ning Gao,Shang-Rong Ji,Yi Wu

BMC cancer 21:765 PubMed34215227

2021

CD36 promotes vasculogenic mimicry in melanoma by mediating adhesion to the extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Carmela Martini,Mark DeNichilo,Danielle P King,Michaelia P Cockshell,Brenton Ebert,Brian Dale,Lisa M Ebert,Anthony Woods,Claudine S Bonder

Analytical methods : advancing methods and applications 13:1191-1203 PubMed33605950

2021

Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Shane Patrick Flanagan,Ronen Fogel,Adrienne Lesley Edkins,Lance St John Ho,Janice Limson

Journal of virology 87:12957-66 PubMed24067955

2013

Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssaviruses in vitro.

Applications

Flow Cyt

Species

Unspecified reactive species

S E Smith,M S Gibson,R S Wash,F Ferrara,E Wright,N Temperton,P Kellam,M Fife

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG H&L (DyLight® 650) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (8)

Product promise