Goat anti-mouse IgG H&L (FITC) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Cited in over 530 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes For PFA fixed tissue see PMID 15716365. |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/500 - 1/2500 | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application IHC (PFA fixed) | Reactivity Expected | Dilution info 1/1000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes (PMID16006199) |
Application ELISA | Reactivity Expected | Dilution info 1/10000 - 1/50000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
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Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Goat anti-mouse IgG H&L (FITC) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Cited in over 530 publications
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution, 0.01% BSA
Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Terms & Conditions.
ab6785 was used at dilution 1/300 with the primary antibody Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade ab6002 in IHC-P. See the review on ab6002.
Anti-Vimentin antibody [RV202] - Cytoskeleton Marker ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
Western blot analysis of ab6785 used as a secondary antibody at 1/1000 dilution for 60 min at RT.
All lanes: Mouse IgG at 50 ng
All lanes: Western blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785) at 1/1000 dilution
Dot blot analysis of ab6785.
All lanes: Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Lane 1: Mouse IgG at 100 ng
Lane 2: Mouse IgG at 33.3 ng
Lane 3: Mouse IgG at 11.1 ng
Lane 4: Mouse IgG at 3.7 ng
Lane 5: Mouse IgG at 1.23 ng
All lanes: Goat Anti-Mouse IgG Fluorescein Conjugated Antibody at 1 µg/mL
ab6785 was used at dilution 1/100 with the primary antibody Anti-HLA G antibody [4H84] ab52455 in Flow Cyt. See the review on ab52455.
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