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Goat anti-mouse IgG H&L (FITC) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Cited in over 530 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (AB6785), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (AB6785), expandable thumbnail
  • Western blot - Goat Anti-Mouse IgG H&L (FITC) (AB6785), expandable thumbnail
  • Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (AB6785), expandable thumbnail
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (AB6785), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
IHC-Fr, Flow Cyt, Dot, IHC-P, IM, IHC (PFA fixed), ICC/IF, ELISA, WB
Clonality
Polyclonal

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Reactivity data

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

For PFA fixed tissue see PMID 15716365.

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/500 - 1/2500
Notes

-

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC (PFA fixed)
Reactivity
Expected
Dilution info
1/1000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

(PMID16006199)

Application
ELISA
Reactivity
Expected
Dilution info
1/10000 - 1/50000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/10000
Notes

-

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Alternative names

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Goat anti-mouse IgG H&L (FITC) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Cited in over 530 publications

Key facts

Description
Goat Anti-Mouse IgG H&L (FITC)
Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
IHC-Fr, Flow Cyt, Dot, IHC-P, IM, IHC (PFA fixed), ICC/IF, ELISA, WB
Clonality
Polyclonal
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution, 0.01% BSA

Purification technique
Affinity purification
Purification notes

Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

    ab6785 was used at dilution 1/300 with the primary antibody Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade ab6002 in IHC-P. See the review on ab6002.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785), expandable thumbnail
    This image is courtesy of a customer review submitted by J Chai.

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

    Anti-Vimentin antibody [RV202] - Cytoskeleton Marker ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

  • Western blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785), expandable thumbnail

    Western blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

    Western blot analysis of ab6785 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

    All lanes: Mouse IgG at 50 ng

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785) at 1/1000 dilution

  • Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785), expandable thumbnail

    Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

    Dot blot analysis of ab6785.

    All lanes: Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

    Lane 1: Mouse IgG at 100 ng

    Lane 2: Mouse IgG at 33.3 ng

    Lane 3: Mouse IgG at 11.1 ng

    Lane 4: Mouse IgG at 3.7 ng

    Lane 5: Mouse IgG at 1.23 ng

    Secondary

    All lanes: Goat Anti-Mouse IgG Fluorescein Conjugated Antibody at 1 µg/mL

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785), expandable thumbnail

    Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

    ab6785 was used at dilution 1/100 with the primary antibody Anti-HLA G antibody [4H84] ab52455 in Flow Cyt. See the review on ab52455.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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