AB97022

Goat Anti-Mouse IgG H&L (FITC)

Name: Goat Anti Mouse (IgG) secondary antibody FITC (ab97022) | Abcam
Brand: Abcam Limited
SKU: AB97022
Price: 265 USD
Availability: InStock

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(42 Publications)

Suitable for IHC-P, ICC/IF, Flow Cyt. Cited in 42 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images

Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (FITC) (AB97022)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (FITC) (AB97022)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :

Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness

Primary antibody : Mouse anti-E-cadherin, 1 : 100
Secondary antibody : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022), 1 : 100
Nuclei were counterstained with DAPI

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110350 PubMed39108722

2024

Heterozygous Gnaq deficiency enhances Ifi202b/IFI16 and NF-κB activation in endothelial cells and exacerbates lupus nephritis pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Yan He,Mengqin Zhang,Jimin Zhang,Wuwei Zhuang,Yuechi Sun,Xing Chen,Hangzhou Fu,Xuanli Tang,Guixiu Shi

Journal of assisted reproduction and genetics 41:2079-2098 PubMed38839698

2024

The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Rahbar,Reza Asadpour,Zohreh Mazaheri

Galen medical journal 12:e2805 PubMed38774852

2024

Evaluation of Apoptosis-related Genes and Hormone Secretion Profiles Using Three Dimensional Culture System of Human Testicular Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Aghbibi Nikmahzar,Farnaz Khadivi,Morteza Koruji,Mehrdad Jahanshahi,Masoomeh Dehghan Tarazjani,Maryam Shabani,Yasaman Abbasi,Mehdi Abbasi

Science signaling 17:eadi9844 PubMed38771918

2024

Condensation of RNase L promotes its rapid activation in response to viral infection in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renee Cusic,James M Burke

Science advances 10:eadk8152 PubMed38295168

2024

G3BP1-dependent condensation of translationally inactive viral RNAs antagonizes infection.

Applications

Unspecified application

Species

Unspecified reactive species

James M Burke,Oshani C Ratnayake,J Monty Watkins,Rushika Perera,Roy Parker

Artificial organs 47:1818-1830 PubMed37698035

2023

Differentiation of human primary testicular cells in the presence of SCF using the organoid culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Aghbibi Nikmahzar,Morteza Koruji,Mehrdad Jahanshahi,Farnaz Khadivi,Maryam Shabani,Sanaz Dehghani,Mehdi Forouzesh,Ayob Jabari,Narjes Feizollahi,Maryam Salem,Nasrin Ghanami Gashti,Yasaman Abbasi,Mehdi Abbasi

BioImpacts : BI 13:97-108 PubMed37193077

2023

Bone marrow mesenchymal stem cells improve cognitive impairments induced by methamphetamine in rats and reduce relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Raheleh Rafaiee,Naghmeh Ahmadiankia,Seyed Abbas Mousavi,Behnaz Jafari,Hamid Kalalian Moghaddam

Iranian journal of basic medical sciences 26:23-29 PubMed36594063

2023

Berberine hydrochloride improves cognitive deficiency through hippocampal up-regulation of neurotrophins following inhalant self-administration of methamphetamine.

Applications

Unspecified application

Species

Unspecified reactive species

Fahimeh Mohseni,Raheleh Rafaiee,Leila Rezaeian,Mohammad Niroumand Sarvandani,Hamid Kalalian Moghaddam

American journal of translational research 14:8903-8917 PubMed36628218

2022

Long non-coding RNA HOTTIP induces inflammation in asthma by promoting EFNA3 transcription by CCCTC-binding factor.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wu,Jinming Liu,Jie Deng,Yuanjing Chen

PLoS pathogens 18:e1010930 PubMed36318584

2022

RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

James M Burke,Nina Ripin,Max B Ferretti,Laura A St Clair,Emma R Worden-Sapper,Fernando Salgado,Sara L Sawyer,Rushika Perera,Kristen W Lynch,Roy Parker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG H&L (FITC)

Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (FITC) (AB97022)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (42)

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