Goat Anti-Mouse IgG H&L (FITC) preadsorbed

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (AB7064)

ab7064 was used at dilution 1/20 with the primary antibody ab7852 in ICC. See the review on ab7852.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (AB7064)

Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells

LAMP1 was detected with a mouse antiLAMP1 antibody in ICC/IF analysis of BS-C-1 cells.

Anti-LAMP1 was visualized using ab7064 at 1/500 diulution.

Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (AB7064)

Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (AB7064)

ab7064 was used at dilution 1/2000 with the primary antibody ab2378 in IHC-P. See the review on ab2378.

• WB

Supplier Data

Western blot - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (AB7064)

Western blot analysis of ab7064 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064) at 1/1000 dilution

false

• Dot

Supplier Data

Dot Blot - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (AB7064)

Dot blot analysis of ab7064. Imaged using the Bio-Rad VersaDoc® 4000 MP.

All lanes:

Dot Blot - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)

Lane 1:

Mouse IgG at 200 ng

Lane 2:

Mouse IgG at 66.67 ng

Lane 3:

Mouse IgG at 22.2 ng

Lane 4:

Mouse IgG at 7.4 ng

Lane 5:

Mouse IgG at 2.47 ng

Secondary

All lanes:

Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescien Conjugated Antibody at 1/1000 dilution

false