Suitable for ELISA, WB, IHC-P, IHC-Fr, ICC. Cited in 3 publications.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/1000.00000 | Notes Substrate:10 mg/ml D(+)glucose, 0.1 mg/ml grade II (Rz=0.6) peroxidase, 0.35 mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm. |
Application WB | Reactivity Reacts | Dilution info 1/400 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
Application ICC | Reactivity Reacts | Dilution info 1/10.00000 - 1/100.00000 | Notes Substrate: 15 mg/ml D(+)glucose, 0.5 mg/ml blue thiazolyl, 0.35 mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness. |
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Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Suitable for ELISA, WB, IHC-P, IHC-Fr, ICC. Cited in 3 publications.
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
ab136783 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
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