Oxidase Goat Anti Mouse (IgG) secondary antibody Glucose (ab136783)

Suitable for ELISA, WB, IHC-P, IHC-Fr, ICC. Cited in 3 publications.

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Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4

Reactivity data

Application	Reactivity	Dilution info	Notes
Application ELISA	Reactivity Reacts	Dilution info 1/100.00000 - 1/1000.00000	Notes Substrate:10 mg/ml D(+)glucose, 0.1 mg/ml grade II (Rz=0.6) peroxidase, 0.35 mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H₂SO₄. Reading at 492 nm.
Application WB	Reactivity Reacts	Dilution info 1/400	Notes -
Application IHC-P	Reactivity Reacts	Dilution info 1/10.00000 - 1/100.00000	Notes -
Application IHC-Fr	Reactivity Reacts	Dilution info 1/10.00000 - 1/100.00000	Notes -
Application ICC	Reactivity Reacts	Dilution info 1/10.00000 - 1/100.00000	Notes Substrate: 15 mg/ml D(+)glucose, 0.5 mg/ml blue thiazolyl, 0.35 mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.

Target data

Suitable for ELISA, WB, IHC-P, IHC-Fr, ICC. Cited in 3 publications.

Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4

Form: Liquid
Storage buffer: Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Purification technique: Affinity purification Immunogen
Purification notes: ab136783 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).

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