Goat anti-mouse IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for IHC, western blot, IP and ELISA.
- Cited in over 1270 publications
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- Aging and disease 15:1499-15072024Senolytic Treatment Improve Small Intestine Regeneration in Aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing-Tian Luo,Yuan-Chun Ye,Wei-Ming Guo,Qing Zhu,Sa-Shuang Wang,Nan Li,Lei Wang,Chun-Sheng Cheng,Gang FanPubMed 37815904
- Journal of nanobiotechnology 22:2332024Comprehensive dry eye therapy: overcoming ocular surface barrier and combating inflammation, oxidation, and mitochondrial damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyou Xia,Yu Zhang,Yangrui Du,Zhigang Wang,Long Cheng,Zhiyu DuPubMed 38725011
- Chemical biology & drug design 103:e145192024Kaempferol attenuates inflammation in lipopolysaccharide-induced gallbladder epithelial cells by inhibiting the MAPK/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Tao Wu,Xin-Xing Lin,Xiao-Lei Yang,Yong Ding,Jia-Liang Wang,Chen-Lu Liu,Wei-Zhou YuPubMed 38570708
- Journal of cancer research and clinical oncology 150:512024Vitamin D-mediated tsRNA-07804 triggers mitochondrial dysfunction and suppresses non-small cell lung cancer progression by targeting CRKL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonggang Liang,Xiaoqiang Zhang,Jinhua Peng,Jing Liu,He Chen,Shanxian GuoPubMed 38289488
- Antioxidants (Basel, Switzerland) 13:2024Relationship between Aspartame-Induced Cerebral Cortex Injury and Oxidative Stress, Inflammation, Mitochondrial Dysfunction, and Apoptosis in Sprague Dawley Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJureeporn U-Pathi,Yen-Chia Yeh,Chia-Wen Chen,Eddy E Owaga,Rong-Hong HsiehPubMed 38275622
- International journal of molecular medicine 53:2024Senolytic combination of dasatinib and quercetin protects against diabetic kidney disease by activating autophagy to alleviate podocyte dedifferentiation via the Notch pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinwang Zhu,Congxiao Zhang,Linlin Liu,Li Xu,Li YaoPubMed 38240118
- Clinical and translational medicine 14:e15392024ARID5B-mediated LINC01128 epigenetically activated pyroptosis and apoptosis by promoting the formation of the BTF3/STAT3 complex in β2GPI/anti-β2GPI-treated monocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Tan,Jiao Qiao,Shuo Yang,Qingchen Wang,Hongchao Liu,Qi Liu,Weimin Feng,Boxin Yang,Zhongxin Li,Liyan CuiPubMed 38224186
- BMC women's health 24:412024Fanconi anemia complementation group D2 promotes sensitivity of endometrial cancer cells to chemotherapeutic agents by inhibiting the ferroptosis pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Hong Lin,Wei-Hong Zeng,Hai-Kun Yang,Li-Shan Huang,Ru Pan,Nan-Xiang LeiPubMed 38218826
- RSC advances 14:2182-21912024Horseradish peroxidase-catalyzed polyacrylamide gels: monitoring their polymerization with BSA-stabilized gold nanoclusters and their functional validation in electrophoresis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Liao,Tao Li,Fengjiao Chen,Shaoying Yan,Liying Zhu,Hua Tang,Dan WangPubMed 38213962
- BMC cancer 24:252024Cyclin B2 impairs the p53 signaling in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinsong Liu,Yong Yuan,Xiaofen Shang,Lu XinPubMed 38166895
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ELISA, IP, WB, IHC-P
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes - |
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Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
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Goat anti-mouse IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for IHC, western blot, IP and ELISA.
- Cited in over 1270 publications
Key facts
- Description
- Goat Anti-Mouse IgG H&L (HRP)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ELISA, IP, WB, IHC-P
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
The antibody used for conjugation reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182017): Chicken IgY, less than 2%. Human IgG, less than 6%. Rabbit IgG, less than 7%. Rat IgG, less than 47%.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 overnight at 4°C. Antibody binding was detected using ab205719, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 52 kDa
Exposure time: 5s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
IHC image of alpha tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution. An HRP-conjugated secondary (ab205719, 1/10000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
IHC image of histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade ab31830 at 1/1000 dilution. An HRP-conjugated secondary (ab205719, 1/10000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 overnight at 4°C. Antibody binding was detected using ab205719, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab205719) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 42 kDa
Exposure time: 10s
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