Goat anti-mouse IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot and ELISA.
- Cited in over 360 publications
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- Orphanet journal of rare diseases 19:152024Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle Bisschoff,Izelle Smuts,Marli Dercksen,Maryke Schoonen,Barend C Vorster,George van der Watt,Careni Spencer,Kireshnee Naidu,Franclo Henning,Surita Meldau,Robert McFarland,Robert W Taylor,Krutik Patel,Mahmoud R Fassad,Jana Vandrovcova,Ronald J A Wanders,Francois H van der WesthuizenPubMed 38221620
- Fluids and barriers of the CNS 21:52024Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodnie Colón Ortiz,Stephen Knerler,Lisa B Fridman,Alicia Mercado,Amira-Storm Price,Jose J Rosado-Franco,Hannah Wilkins,Bianca R Flores,Benjamin C Orsburn,Dionna W WilliamsPubMed 38200564
- PLoS genetics 19:e10111092023The Muscleblind-like protein MBL-1 regulates microRNA expression in Caenorhabditis elegans through an evolutionarily conserved autoregulatory mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJens Verbeeren,Joana Teixeira,Susana M D A GarciaPubMed 38134228
- Oncology letters 27:162023TREM1 is involved in the mechanism between asthma and lung cancer by regulating the Toll‑like receptor signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhulin Yan,Wei Zhang,Keyu SunPubMed 38028174
- Nature communications 14:75322023Vesicles driven by dynein and kinesin exhibit directional reversals without regulators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshwin I D'Souza,Rahul Grover,Gina A Monzon,Ludger Santen,Stefan DiezPubMed 37985763
- Development (Cambridge, England) 150:2023Dysregulation of the endoplasmic reticulum blocks recruitment of centrosome-associated proteins resulting in mitotic failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine R Rollins,J Todd BlankenshipPubMed 37971218
- Nature communications 14:73492023TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Liu,Tatt Jhong Haw,Malcolm R Starkey,Ashleigh M Philp,Stelios Pavlidis,Christina Nalkurthi,Prema M Nair,Henry M Gomez,Irwan Hanish,Alan Cy Hsu,Elinor Hortle,Sophie Pickles,Joselyn Rojas-Quintero,Raul San Jose Estepar,Jacqueline E Marshall,Richard Y Kim,Adam M Collison,Joerg Mattes,Sobia Idrees,Alen Faiz,Nicole G Hansbro,Ryutaro Fukui,Yusuke Murakami,Hong Sheng Cheng,Nguan Soon Tan,Sanjay H Chotirmall,Jay C Horvat,Paul S Foster,Brian Gg Oliver,Francesca Polverino,Antonio Ieni,Francesco Monaco,Gaetano Caramori,Sukhwinder S Sohal,Ken R Bracke,Peter A Wark,Ian M Adcock,Kensuke Miyake,Don D Sin,Philip M HansbroPubMed 37963864
- Pediatric research 95:996-10082023microRNA profilings identify plasma biomarkers and targets associated with pediatric epilepsy patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Wang,Xulai Shi,Ping-Ping Li,Li Gao,Yueyuan Zhou,Luyao Li,Hao Ye,Xiaoqin Fu,Peijun LiPubMed 37884644
- Molecular therapy. Nucleic acids 34:1020602023Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoko Shiba,Xiao Yang,Mitsuto Sato,Shin Kadota,Yota Suzuki,Masahiro Agata,Kohei Nagamine,Masaki Izumi,Yusuke Honda,Tomoya Koganehira,Hideki Kobayashi,Hajime Ichimura,Shinichiro Chuma,Junichi Nakai,Shugo Tohyama,Keiichi Fukuda,Daigo Miyazaki,Akinori Nakamura,Yuji ShibaPubMed 38028197
- Journal of neuroinflammation 20:2302023Stabilizing histamine release in gut mast cells mitigates peripheral and central inflammation after stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria P Blasco Conesa,Frank W Blixt,Pedram Peesh,Romeesa Khan,Janelle Korf,Juneyoung Lee,Gayathri Jagadeesan,Alexander Andersohn,Tushar K Das,Chunfeng Tan,Claudia Di Gesu,Gabriela Delevati Colpo,Jose Félix Moruno-Manchón,Louise D McCullough,Robert Bryan,Bhanu P GaneshPubMed 37805585
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Goat anti-mouse IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot and ELISA.
- Cited in over 360 publications
Key facts
- Description
- Goat Anti-Mouse IgG H&L (HRP)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Part of the AbExcel range.
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Full details and terms and conditions can be found here:
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6 product images
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1 μg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 μg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 secondsSecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
This image is courtesy of an anonymous customer review.Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97023, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (Anti-hnRNP M3-M4 antibody [2A6-2H3] ab128143) at 1/1000 dilution
Lane 1: HEK293T whole cell lysate at 5 µg
Lane 2: LNCaP whole cell lysate at 5 µg
Lane 3: A-549 whole cell lysate at 5 µg
Lane 4: MCF-7 whole cell lysate at 5 µg
Lane 5: K-562 whole cell lysate at 5 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 3s
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (Anti-hnRNP M3-M4 antibody [2A6-2H3] ab128143) at 1/1000 dilution
Lane 1: BW5147.3 whole cell lysate at 10 µg
Lane 2: CT26 whole cell lysate at 10 µg
Lane 3: CH27 whole cell lysate at 10 µg
Lane 4: TCMK-1 whole cell lysate at 10 µg
Lane 5: NIH 3T3 whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 10s
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
All lanes: Western blot - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] ab128175) at 1/1000 dilution
Lane 1: Hep-G2 whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 50 µg
Lane 3: HEK293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Lane 5: NIH 3T3 whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)
Developed using the ECL technique.
Predicted band size: 66 kDa, 70 kDa
Observed band size: 70 kDa, 66 kDa
Exposure time: 10s
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