Goat Anti-Mouse IgG H&L (HRP)

• WB

Unknown

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB97023)

All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/20000 dilution

Developed using the ECL technique

Performed under reducing conditions.

Exposure time : 20 seconds

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

false

• WB

Supplier Data

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB97023)

All lanes:

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (<a href='/en-us/products/primary-antibodies/hnrnp-m3-m4-antibody-2a6-2h3-ab128143'>ab128143</a>) at 1/1000 dilution

Lane 1:

HEK293T whole cell lysate at 5 µg

Lane 2:

LNCaP whole cell lysate at 5 µg

Lane 3:

A-549 whole cell lysate at 5 µg

Lane 4:

MCF-7 whole cell lysate at 5 µg

Lane 5:

K-562 whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

Predicted band size: 77 kDa

Observed band size: 80 kDa

true

Exposure time: 3s

• WB

Supplier Data

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB97023)

All lanes:

Western blot - Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (<a href='/en-us/products/primary-antibodies/igf2bp2-imp-2-antibody-1e301e5-ab128175'>ab128175</a>) at 1/1000 dilution

Lane 1:

Hep-G2 whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

HEK293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

Predicted band size: 66 kDa,70 kDa

Observed band size: 70 kDa,66 kDa

true

Exposure time: 10s

• WB

Supplier Data

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB97023)

All lanes:

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (<a href='/en-us/products/primary-antibodies/hnrnp-m3-m4-antibody-2a6-2h3-ab128143'>ab128143</a>) at 1/1000 dilution

Lane 1:

BW5147.3 whole cell lysate at 10 µg

Lane 2:

CT26 whole cell lysate at 10 µg

Lane 3:

CH27 whole cell lysate at 10 µg

Lane 4:

TCMK-1 whole cell lysate at 10 µg

Lane 5:

NIH 3T3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

Predicted band size: 77 kDa

Observed band size: 80 kDa

true

Exposure time: 10s

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (AB97023)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97023, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

AbReview32272****

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB97023)

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab97023)

true

Exposure time: 3min

This image is courtesy of an anonymous Abreview.