Goat Anti-Mouse IgG H&L (HRP polymer) is a secondary antibody that is ideal for tissue imaging. Suitable for IHC and more.
- Reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins
- No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit
- Cited in over 10 publications
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Publications
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- Biology of sex differences 14:892023Sex and interspecies differences in ESR2-expressing cell distributions in mouse and rat brains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Morishita,Shimpei Higo,Kinuyo Iwata,Hirotaka IshiiPubMed 38111056
- Nature communications 13:14872022Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFotis Nikolos,Kazukuni Hayashi,Xen Ping Hoi,Mark Ellie Alonzo,Qianxing Mo,Armine Kasabyan,Hideki Furuya,Jane Trepel,Dolores Di Vizio,Jlenia Guarnerio,Dan Theodorescu,Charles Rosser,Andrea Apolo,Matthew Galsky,Keith Syson ChanPubMed 35347124
- Brain and behavior 12:e25222022Protective role of activating transcription factor 3 against neuronal damage in rats with cerebral ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Ma,Gaixia Li,Xiuxin FuPubMed 35263513
- Current protocols 2:e3572022Establishing an In Vitro 3D Spheroid Model to Study Medulloblastoma Drug Response and Tumor Dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie J Roper,Beth CoylePubMed 35080813
- Cancer medicine 10:6442-64552021Inhibition of lipid metabolism exerts antitumor effects on rhabdomyosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Miyagaki,Ken Kikuchi,Jun Mori,Gary D Lopaschuk,Tomoko Iehara,Hajime HosoiPubMed 34472721
- Frontiers in pharmacology 11:6283142021Neobavaisoflavone Induces Bilirubin Metabolizing Enzyme UGT1A1 via PPARα and PPARγ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Di Zhu,Xiao-Qing Guan,Jing Chen,Sheng Peng,Moshe Finel,Ying-Yuan Zhao,Rui-Min Wang,Hui-Chang Bi,Ming Lei,Dan-Dan Wang,Guang-Bo GePubMed 33628187
- Scientific reports 11:425920213D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie J Roper,Franziska Linke,Paul J Scotting,Beth CoylePubMed 33608621
- Gastroenterology 160:1224-1239.e302020The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Kobayashi,Krystyna A Gieniec,Josephine A Wright,Tongtong Wang,Naoya Asai,Yasuyuki Mizutani,Tadashi Lida,Ryota Ando,Nobumi Suzuki,Tamsin R M Lannagan,Jia Q Ng,Akitoshi Hara,Yukihiro Shiraki,Shinji Mii,Mari Ichinose,Laura Vrbanac,Matthew J Lawrence,Tarik Sammour,Kay Uehara,Gareth Davies,Leszek Lisowski,Ian E Alexander,Yoku Hayakawa,Lisa M Butler,Andrew C W Zannettino,M Omar Din,Jeff Hasty,Alastair D Burt,Simon J Leedham,Anil K Rustgi,Siddhartha Mukherjee,Timothy C Wang,Atsushi Enomoto,Masahide Takahashi,Daniel L Worthley,Susan L WoodsPubMed 33197448
- Cancer chemotherapy and pharmacology 86:361-3742020miR-331-3p is involved in glucocorticoid resistance reversion by rapamycin through suppression of the MAPK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianna Lucafò et. al.PubMed 32776229
- Iranian journal of basic medical sciences 22:722-7282020Irisin protects the substantia nigra dopaminergic neurons in the rat model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSam Zarbakhsh,Manouchehr Safari,Mohammad Reza Aldaghi,Hamid Reza Sameni,Laya Ghahari,Younes Khaleghi Lagmouj,Khojasteh Rahimi Jaberi,Houman ParsaiePubMed 32373292
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP polymer
- Applications
- Ie, IHC-P
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01868
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Ie | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes This product is provided in a pre-diluted, ready-to-use format. Incubate 150 μl per slide for 1 hour. |
Associated Products
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6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Goat Anti-Mouse IgG H&L (HRP polymer) is a secondary antibody that is ideal for tissue imaging. Suitable for IHC and more.
- Reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins
- No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit
- Cited in over 10 publications
Key facts
- Description
- Goat Anti-Mouse IgG H&L (HRP polymer)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP polymer
- Applications
- Ie, IHC-P
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01868
- Specificity
Based on IEP, ab214879 reacts with heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins. No cross-reactivity is observed to non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig or rabbit.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.1% Proclin 300
Constituents: 98% PBS, 1% BSA- Purification technique
- Affinity purification
- Purification notes
ab214879 was affinity purified using solid phase Mouse IgG. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody reacts with the heavy and light chains of Mouse IgG
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10 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of Triple positive breast cancer tissue stained with Ki-67 and detected with ab214879.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue using Anti-SOX10 antibody [SOX10/991] ab218522 at a 1/2000 dilution followed by a ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Nuclear staining in mouse cerebrum.
The section was incubated with Anti-SOX10 antibody [SOX10/991] ab218522 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded Human breast tissue using Anti-SOX10 antibody [SOX10/991] ab218522 at a 1/1000 dilution followed by a ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Nuclear staining in human breast.
The section was incubated with Anti-SOX10 antibody [SOX10/991] ab218522 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Tau (phospho S396) with Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free ab302491 at 1/20000 (0.05 ug/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Positive staining on human cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free ab302491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Tau (phospho S396) with Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free ab302491 at 1/20000 (0.05 ug/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Positive staining on human breast carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free ab302491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded human tissues labeling Hsp27 with Anti-Hsp27 antibody [G3.1] ab2790 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with Anti-Hsp27 antibody [G3.1] ab2790 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC2 with Anti-MUC2 antibody [996/1] ab11197 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with Anti-MUC2 antibody [996/1] ab11197 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with Anti-Human IgG antibody [IG266] ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Cytoplasmic staining in plasma of human colon.
The section was incubated with Anti-Human IgG antibody [IG266] ab200699 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue using Anti-SOX10 antibody [SOX10/991] ab218522 at a 1/2000 dilution followed by a ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Nuclear staining in rat cerebrum.
The section was incubated with Anti-SOX10 antibody [SOX10/991] ab218522 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Immunohistochemical analysis of paraffin-embedded Human pituitary gland tissue labeling ACTH with Anti-ACTH antibody [AH26] ab233953 at 1/5000 (0.233 µg/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Positive staining on human pituitary gland. The section was incubated with Anti-ACTH antibody [AH26] ab233953 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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