AB7063

Goat Anti-Mouse IgG H&L preadsorbed

Name: Goat Anti-Mouse IgG preadsorbed secondary antibody (ab7063) | Abcam
Brand: Abcam Limited
SKU: AB7063
Price: 220 USD
Availability: InStock

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(13 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 13 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

ELISA - Goat Anti-Mouse IgG H&L preadsorbed (AB7063)

ELISA

Supplier Data

ELISA - Goat Anti-Mouse IgG H&L preadsorbed (AB7063)

ELISA results of ab7063 against purified Mouse IgG mx10. Each well was coated in duplicate with 1.0 µg of Mouse IgG [Red Line], Bovine IgG (p/n 001-0102) [Green Line], Chicken IgG [Blue Line], Goat IgG [Purple Line], Guinea Pig IgG, Hamster IgG, Horse IgG, Human IgG, Rabbit IgG, Rat IgG, and Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Applications

ELISA, WB, IP, ICC/IF, IHC-P, IHC-Fr, Conjugation, ID

applications

Clonality

Polyclonal

Immunogen

Native Full Length Protein corresponding to Mouse Ighg1.

P01868

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/5000 - 1/20000", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/20000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50000 - 1/100000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & medicine 22: PubMed40434360

2025

Nuclear PHGDH regulates macrophage polarization through transcriptional repression of and in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Wang,Xin Du,Zhiren Han,Jiaxin Zhong,Jiayu Yuan,Lin Jiang,Beinan Han,Wenkui Fu,Hongde Li,Hai Hu,Zhenkun Na

PeerJ 12:e18227 PubMed39434797

2024

ALKBH5 modulation of ferroptosis in recurrent miscarriage: implications in cytotrophoblast dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanmei Qin,Jiayi Wu,Xiaowei Wei,Xueqing Liu,Yi Lin

The Journal of infectious diseases 231:e244-e253 PubMed39126323

2024

Characterization of the Spatiotemporal Localization of a Pan-Mucorales-Specific Antigen During Germination and Immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa C Hudson,Dora E Corzo-Léon,Iana Kalinina,Duncan Wilson,Christopher R Thornton,Adilia Warris,Elizabeth R Ballou

Translational oncology 46:101996 PubMed38795560

2024

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingke Li,Zhiwu Wang,Jian Wang,Jiangong Wang,Xuan Zheng,Dan Li,Zhuo Wang,Jingwu Li,Yufeng Li

Phytotherapy research : PTR 38:1745-1760 PubMed37740455

2023

Ginsenoside RG1-induced mesenchymal stem cells alleviate diabetic cardiomyopathy through secreting exosomal circNOTCH1 to promote macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Zhen,Jinping Bai,Jia Liu,Hongbo Men,Haitao Yu

Frontiers in immunology 14:1196350 PubMed37465664

2023

Reduning alleviates sepsis-induced acute lung injury by reducing apoptosis of pulmonary microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Wang,Zhe Guo,Xuesong Wang,Haiyan Liao,Feng Chen,Yuxin Liu,Zhong Wang

Cellular and molecular life sciences : CMLS 80:156 PubMed37208565

2023

CSNK1D-mediated phosphorylation of HNRNPA2B1 induces miR-25-3p/miR-93-5p maturation to promote prostate cancer cell proliferation and migration through mA-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Qi,Wenyi Shen,Xiyi Wei,Yifei Cheng,Fan Xu,Yuxiao Zheng,Lu Li,Chao Qin,Xiao Li

Molecular medicine reports 26: PubMed36004465

2022

Angiotensin II receptor type 1 blocker candesartan improves morphine tolerance by reducing morphine‑induced inflammatory response and cellular activation of BV2 cells via the PPARγ/AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxin Zhao,Feiyan Shen,Jixiang Yao,Shanshan Su,Zhongmin Zhao

Cell transplantation 31:9636897221108192 PubMed35838296

2022

MiR-199-3p Suppressed Inflammatory Response by Targeting MECP2 to Alleviate TRX-Induced PHN in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijian Wang,Wei Shen,Mengye Zhu,Mu Xu,Mizhen Qiu,Daying Zhang,Shibiao Chen

Small methods 6:e2200152 PubMed35451210

2022

Protein Ligand Nanopattern Size Selects for Cellular Adhesion via Hemidesmosomes over Focal Adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Sadegh Ghorbani,Ali Shahrokhtash,Julien E Gautrot,Duncan S Sutherland

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG H&L preadsorbed

ELISA - Goat Anti-Mouse IgG H&L preadsorbed (AB7063)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Applications

Clonality

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (13)

Product promise