Goat Anti-Mouse IgG H&L (TRITC)

3 product images

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

  Overlay histogram showing Jurkat cells stained with Anti-CD3 antibody [MEM-57] ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 antibody [MEM-57] ab8090, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.

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  Western blot - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

  Western blot analysis of ab6786 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

  All lanes: Mouse IgG at 50 ng

  Secondary

  All lanes: Western blot - Goat Anti-Mouse IgG H&L (TRITC) (ab6786) at 1/1000 dilution

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  Dot Blot - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

  Dot blot analysis of ab6786.

  All lanes: Dot Blot - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

  Lane 1: Mouse IgG at 50 ng

  Lane 2: Mouse IgG at 16.67 ng

  Lane 3: Mouse IgG at 5.56 ng

  Lane 4: Mouse IgG at 1.85 ng

  Lane 5: Mouse IgG at 0.62 ng

  Secondary

  All lanes: Rhodamine Conjugated Goat Anti-Mouse IgG secondary antibody at 1/1000 dilution