Suitable for ICC, ELISA, IHC-P, WB. Ideal for western blot. Cited in 130 publications.
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- NPJ vaccines 8:1872023A Pvs25 mRNA vaccine induces complete and durable transmission-blocking immunity to Plasmodium vivax.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNawapol Kunkeaw,Wang Nguitragool,Eizo Takashima,Niwat Kangwanrangsan,Hiromi Muramatsu,Mayumi Tachibana,Tomoko Ishino,Paulo J C Lin,Ying K Tam,Sathit Pichyangkul,Takafumi Tsuboi,Norbert Pardi,Jetsumon SattabongkotPubMed 38092803
- Neoplasma 70:670-6822023Exploring the expression of SNHG1 and its effect on the PI3K-AKT axis in nasopharyngeal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Yang,Yan-Ping Yang,Mei-Ling Yi,Fang-Ting Huang,Xia Zhu,Guang-Wu HuangPubMed 38053377
- Diseases (Basel, Switzerland) 11:2023Systematic Evaluation of the Distribution of Immune Cells following Subcutaneous Administration of Haemophilus Influenzae Type B Vaccine to Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao He,Yuxiu Zhao,Hongyang Liang,Xue Wang,Haoyue Lan,Dongyang Tian,Yan Li,Hui WangPubMed 37873783
- PLoS neglected tropical diseases 17:e00116252023Excretory/Secretory Products from Schistosoma japonicum Eggs Alleviate Ovalbumin-Induced Allergic Airway Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhidan Li,Xiaoling Wang,Wei Zhang,Wenbin Yang,Bin Xu,Wei HuPubMed 37788409
- Toxins 15:2023PLGA Nanoparticle-Based Dissolving Microneedle Vaccine of ε Toxin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wan,Yue Li,Jing Wang,Zhiying Jin,Wenwen Xin,Lin Kang,Junhong Wang,Xiaoyang Li,Yakun Cao,Hao Yang,Jinglin Wang,Shan GaoPubMed 37505730
- iScience 26:1071202023Comparison of DNA vaccines with AS03 as an adjuvant and an mRNA vaccine against SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPraveen Neeli,Dafei Chai,Xu Wang,Navid Sobhani,George Udeani,Yong LiPubMed 37361876
- Epigenetics & chromatin 16:262023PARP-dependent and NAT10-independent acetylation of N4-cytidine in RNA appears in UV-damaged chromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlena Svobodová Kovaříková,Lenka Stixová,Aleš Kovařík,Eva BártováPubMed 37322549
- NPJ vaccines 8:882023A mosquito AgTRIO mRNA vaccine contributes to immunity against malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Min Chuang,Mohamad-Gabriel Alameh,Selma Abouneameh,Hamidah Raduwan,Michel Ledizet,Drew Weissman,Erol FikrigPubMed 37286568
- Cells 12:2023Intermittent Theta Burst Stimulation Improves Motor and Behavioral Dysfunction through Modulation of NMDA Receptor Subunit Composition in Experimental Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilica Zeljkovic Jovanovic,Jelena Stanojevic,Ivana Stevanovic,Andjela Stekic,Samuel J Bolland,Nebojsa Jasnic,Milica Ninkovic,Marina Zaric Kontic,Tihomir V Ilic,Jennifer Rodger,Nadezda Nedeljkovic,Milorad DragicPubMed 37296646
- Nature immunology 24:1124-11372023Spatial dysregulation of T follicular helper cells impairs vaccine responses in aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyssa Silva-Cayetano,Sigrid Fra-Bido,Philippe A Robert,Silvia Innocentin,Alice R Burton,Emily M Watson,Jia Le Lee,Louise M C Webb,William S Foster,Ross C J McKenzie,Alexandre Bignon,Ine Vanderleyden,Dominik Alterauge,Julia P Lemos,Edward J Carr,Danika L Hill,Isabella Cinti,Karl Balabanian,Dirk Baumjohann,Marion Espeli,Michael Meyer-Hermann,Alice E Denton,Michelle A LintermanPubMed 37217705
Key facts
- Host species
Goat
- Target species
Mouse
- Target isotype
IgG1
- Target specificity
Heavy chain
- Conjugation
HRP
- Applications
ICC, ELISA, IHC-P, WB
- Clonality
Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method. |
Targets
Ig gamma-1 chain C region, membrane-bound form
Alternative names
Igh-4, Ighg1, Igh-4, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for ICC, ELISA, IHC-P, WB. Ideal for western blot. Cited in 130 publications.
Key facts
- Description
Goat Anti-Mouse IgG1 (HRP)
- Host species
Goat
- Target species
Mouse
- Target isotype
IgG1
- Target specificity
Heavy chain
- Conjugation
HRP
- Applications
ICC, ELISA, IHC-P, WB
- Clonality
Polyclonal
- Pre-adsorbed
No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Product promise
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) (ab97240)
IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-Histone H1.0 antibody [27] ab11080, 7.5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Image from Arnò B et al., PLOS One, Fig. 2.; doi: 10.1371/journal.pone.0068404 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Western blot - Goat Anti-Mouse IgG1 (HRP) (ab97240)
The band corresponding to the enzyme–substrate cleaved complex, has been indicated by an asterisk.
All lanes: Anti-Topoisomerase I antibody (ab58313)
Lane 1: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 1 minute.
Lane 2: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 2 minutes.
Lane 3: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 4 minutes.
Lane 4: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 8 minutes.
Lane 5: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 1 minute.
Lane 6: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 2 minutes.
Lane 7: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 4 minutes.
Lane 8: Products generated by incubation of hTop1 with [γ -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 8 minutes.
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG1 (HRP) (ab97240)
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