AB98693

Goat Anti-Mouse IgG1 (HRP) preadsorbed

Name: Goat Anti-Mouse IgG1 (HRP) preadsorbed secondary antibody (ab98693) | Abcam
Brand: Abcam Limited
SKU: AB98693
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 23 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (AB98693)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (AB98693)

IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab98693, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG1

Target specificity

Heavy chain

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ELISA, IHC-P, ICC, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1.

Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins.

Less than 1% cross reactivity to human and rat IgG1 was detected. This antibody may cross react with IgG1 from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/20000", "notes":"Colorimetric1/5000 - 1/25000 Chemiluminescent" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"Similar dilution to primary antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG1 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:32134 PubMed40890409

2025

The administration of passive and active immunotherapy against Syntenin-1 decreased the tumoral growth and pulmonary metastasis in a murine model of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

María Lilia Nicolás-Morales,Víctor Manuel Luna-Pineda,Carlos Alberto Serrano-Bello,Miguel David Guerrero-Macedonio,Cynthia Rodríguez-Nava,Isela Parra-Rojas,Mónica Espinoza-Rojo,Eugenia Flores-Alfaro,Luz Del Carmen Alarcón-Romero,Amalia Vences-Velázquez,Karen Cortés-Sarabia

Skin health and disease 4:e394 PubMed39355740

2024

Pigment epithelium derived factor drives melanocyte proliferation and migration in neurofibromatosis café au lait macules.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Lovatt,Megan Williams,Alex Gibbs,Abdullahi Mukhtar,Huw J Morgan,Simone Lanfredini,Carlotta Olivero,Gill Spurlock,Sally Davies,Charlotte Philpott,Hannah Tovell,Peter Turnpenny,Dilair Baban,Sam Knight,Hilde Brems,Julian R Sampson,Eric Legius,Meena Upadhyaya,Girish K Patel

Parasites & vectors 17:377 PubMed39237959

2024

Live-attenuated PruΔgra72 strain of Toxoplasma gondii induces strong protective immunity against acute and chronic toxoplasmosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Yu Kang,Ze-Xuan Wu,Shu-Feng Yang,Yu-Yang Tian,Xing-Quan Zhu,Xiao-Nan Zheng

Vaccines 12: PubMed39066391

2024

KD-409, a Respiratory Syncytial Virus FG Chimeric Protein without the CX3C Chemokine Motif, Is an Efficient Respiratory Syncytial Virus Vaccine Preparation for Passive and Active Immunization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Yamaue,Masaharu Torikai,Madoka Terashima,Hiroaki Mori

Nature nanotechnology 18:1105-1114 PubMed37365276

2023

Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexiang Han,Mohamad-Gabriel Alameh,Kamila Butowska,James J Knox,Kendall Lundgreen,Majed Ghattas,Ningqiang Gong,Lulu Xue,Ying Xu,Marc Lavertu,Paul Bates,Junchao Xu,Guangjun Nie,Yi Zhong,Drew Weissman,Michael J Mitchell

Journal of nanobiotechnology 21:175 PubMed37264420

2023

A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xiang Jia,Sen-Lin Peng,Ndayambaje Yvan Kalisa,Qiang Chao,Zhifang Zhou,Xiao-Dong Gao,Ning Wang

Cell reports 42:112182 PubMed36934325

2023

Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Itoh,Vibhu Sahni,Sara J Shnider,Holly McKee,Jeffrey D Macklis

EBioMedicine 89:104485 PubMed36857860

2023

COVID-19 mRNA vaccine protects against SARS-CoV-2 Omicron BA.1 infection in diet-induced obese mice through boosting host innate antiviral responses.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Chen,Wenchen Song,Can Li,Jiaxuan Wang,Feifei Liu,Zhanhong Ye,Peidi Ren,Yihan Tong,Junhua Li,Zhihua Ou,Andrew Chak-Yiu Lee,Jian-Piao Cai,Bosco Ho-Yin Wong,Jasper Fuk-Woo Chan,Kwok-Yung Yuen,Anna Jin-Xia Zhang,Hin Chu

Biomaterials science 10:6968-6979 PubMed36222485

2022

assessment of triazine lipid nanoparticles as transfection agents for plasmid DNA.

Applications

Unspecified application

Species

Unspecified reactive species

David Nardo,Michelle G Pitts,Rupinder Kaur,Vincent J Venditto

Acta pharmaceutica Sinica. B 13:2219-2233 PubMed35846427

2022

Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Fuhua Wu,Shuang Luo,Yongshun Zhang,Yangsen Ou,Hairui Wang,Zhaofei Guo,Chunting He,Shuting Bai,Penghui He,Min Jiang,Xiaoyan Chen,Guangsheng Du,Xun Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG1 (HRP) preadsorbed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (AB98693)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (23)

Product promise