Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 19 publications.
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- Nature nanotechnology 18:1105-11142023Adjuvant lipidoid-substituted lipid nanoparticles augment the immunogenicity of SARS-CoV-2 mRNA vaccines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuexiang Han,Mohamad-Gabriel Alameh,Kamila Butowska,James J Knox,Kendall Lundgreen,Majed Ghattas,Ningqiang Gong,Lulu Xue,Ying Xu,Marc Lavertu,Paul Bates,Junchao Xu,Guangjun Nie,Yi Zhong,Drew Weissman,Michael J MitchellPubMed 37365276
- Journal of nanobiotechnology 21:1752023A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Xiang Jia,Sen-Lin Peng,Ndayambaje Yvan Kalisa,Qiang Chao,Zhifang Zhou,Xiao-Dong Gao,Ning WangPubMed 37264420
- Cell reports 42:1121822023Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Itoh,Vibhu Sahni,Sara J Shnider,Holly McKee,Jeffrey D MacklisPubMed 36934325
- EBioMedicine 89:1044852023COVID-19 mRNA vaccine protects against SARS-CoV-2 Omicron BA.1 infection in diet-induced obese mice through boosting host innate antiviral responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanxia Chen,Wenchen Song,Can Li,Jiaxuan Wang,Feifei Liu,Zhanhong Ye,Peidi Ren,Yihan Tong,Junhua Li,Zhihua Ou,Andrew Chak-Yiu Lee,Jian-Piao Cai,Bosco Ho-Yin Wong,Jasper Fuk-Woo Chan,Kwok-Yung Yuen,Anna Jin-Xia Zhang,Hin ChuPubMed 36857860
- Biomaterials science 10:6968-69792022assessment of triazine lipid nanoparticles as transfection agents for plasmid DNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Nardo,Michelle G Pitts,Rupinder Kaur,Vincent J VendittoPubMed 36222485
- Acta pharmaceutica Sinica. B 13:2219-22332022Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuhua Wu,Shuang Luo,Yongshun Zhang,Yangsen Ou,Hairui Wang,Zhaofei Guo,Chunting He,Shuting Bai,Penghui He,Min Jiang,Xiaoyan Chen,Guangsheng Du,Xun SunPubMed 35846427
- iScience 25:1044562022Mechanistic elucidation of freezing-induced surface decomposition of aluminum oxyhydroxide adjuvant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahuan Li,Ge Yu,Zhihui Liang,Min Li,Chen Chen,Xin Li,Yiyang Guo,Cheng Yang,Yang Liu,Caiqiao Zhang,Weiting Zhang,Jiaxu Liu,Xuehu Ma,Changying Xue,Bingbing SunPubMed 35874920
- Nature communications 13:8242022Development of a skin- and neuro-attenuated live vaccine for varicella.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Dequan Pan,Wenkun Fu,Xiangzhong Ye,Jinle Han,Lianwei Yang,Jizong Jia,Jian Liu,Rui Zhu,Yali Zhang,Che Liu,Jianghui Ye,Anca Selariu,Yuqiong Que,Qinjian Zhao,Ting Wu,Yimin Li,Jun Zhang,Tong Cheng,Hua Zhu,Ningshao XiaPubMed 35149692
- Marine drugs 18:2020Viridicatol Isolated from Deep-Sea Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhendan Shu et. al.PubMed 33081290
- ImmunoHorizons 4:455-4632020Autoantibody Responses to Apolipoprotein A-I Are Not Diet- or Sex-Linked in C57BL/6 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle G Pitts et. al.PubMed 32759326
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG1
- Target specificity
- Heavy chain
- Minimal cross-reactivity
- Rat, Human
- Conjugation
- HRP
- Applications
- ICC, IHC-P, WB, ELISA
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes Colorimetric1/5000 - 1/25000 Chemiluminescent |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes Similar dilution to primary antibody. |
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 19 publications.
Key facts
- Description
- Goat Anti-Mouse IgG1 (HRP) preadsorbed
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG1
- Target specificity
- Heavy chain
- Minimal cross-reactivity
- Rat, Human
- Conjugation
- HRP
- Applications
- ICC, IHC-P, WB, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1.
Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins.
Less than 1% cross reactivity to human and rat IgG1 was detected. This antibody may cross react with IgG1 from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG1 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG1 (HRP) preadsorbed (ab98693)
IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-Histone H1.0 antibody [27] ab11080, 7.5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab98693, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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