Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 75 publications.
Goat
Mouse
IgG2a
Heavy chain
HRP
ICC, IHC-P, ELISA, WB
Polyclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes Similar dilution to primary antibody. |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000 |
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 75 publications.
Goat Anti-Mouse IgG2a heavy chain (HRP)
Goat
Mouse
IgG2a
Heavy chain
HRP
ICC, IHC-P, ELISA, WB
Polyclonal
No
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%.
IgG
Liquid
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA
Affinity purification Immunogen
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Blue Ice
+4°C
+4°C
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IHC image of SSB staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-SSB antibody [mAbcam75927] ab75927, 0.5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97245, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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