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Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 75 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (AB97245), expandable thumbnail

Publications

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG2a

Target specificity

Heavy chain

Conjugation

HRP

Applications

ICC, IHC-P, ELISA, WB

Clonality

Polyclonal

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Reactivity data

Application

ICC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

1/200.00000 - 1/2000.00000

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/10000.00000 - 1/100000.00000

Notes

Similar dilution to primary antibody.

Application

WB

Reactivity

Reacts

Dilution info

1/2000.00000 - 1/25000.00000

Notes

Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000

Recommended products

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 75 publications.

Key facts

Description

Goat Anti-Mouse IgG2a heavy chain (HRP)

Host species

Goat

Target species

Mouse

Target isotype

IgG2a

Target specificity

Heavy chain

Conjugation

HRP

Applications

ICC, IHC-P, ELISA, WB

Clonality

Polyclonal

Pre-adsorbed

No

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with other immunoglobulins is less than 2%.

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Product promise

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Full details and terms and conditions can be found here:
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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245)

    IHC image of SSB staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-SSB antibody [mAbcam75927] ab75927, 0.5μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97245, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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