AB98698

Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed

Name: Goat Anti Mouse (IgG2a heavy chain) secondary antibody preadsorbed HRP (ab98698)
Brand: Abcam Limited
SKU: AB98698
Price: 265 USD
Availability: InStock

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(13 Publications)

Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 13 publications.

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG2a

Target specificity

Heavy chain

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ELISA, ICC, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2a was detected. This antibody may cross react with IgG2a from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/20000", "notes":"Colorimetric1/5000 - 1/25000 Chemiluminescent" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"Primary" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2a was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Mouse IgG2a

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:32134 PubMed40890409

2025

The administration of passive and active immunotherapy against Syntenin-1 decreased the tumoral growth and pulmonary metastasis in a murine model of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

María Lilia Nicolás-Morales,Víctor Manuel Luna-Pineda,Carlos Alberto Serrano-Bello,Miguel David Guerrero-Macedonio,Cynthia Rodríguez-Nava,Isela Parra-Rojas,Mónica Espinoza-Rojo,Eugenia Flores-Alfaro,Luz Del Carmen Alarcón-Romero,Amalia Vences-Velázquez,Karen Cortés-Sarabia

Parasites & vectors 17:377 PubMed39237959

2024

Live-attenuated PruΔgra72 strain of Toxoplasma gondii induces strong protective immunity against acute and chronic toxoplasmosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Yu Kang,Ze-Xuan Wu,Shu-Feng Yang,Yu-Yang Tian,Xing-Quan Zhu,Xiao-Nan Zheng

Vaccines 12: PubMed39066391

2024

KD-409, a Respiratory Syncytial Virus FG Chimeric Protein without the CX3C Chemokine Motif, Is an Efficient Respiratory Syncytial Virus Vaccine Preparation for Passive and Active Immunization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Yamaue,Masaharu Torikai,Madoka Terashima,Hiroaki Mori

Journal of extracellular vesicles 12:e12354 PubMed37553837

2023

Endocytosis of red blood cell extracellular vesicles by macrophages leads to cytoplasmic heme release and prevents foam cell formation in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thach Tuan Pham,Anh Hong Le,Cong Phi Dang,Suet Yen Chong,Dang Vinh Do,Boya Peng,Migara Kavishka Jayasinghe,Hong Boon Ong,Dong Van Hoang,Roma Anne Louise,Yuin-Han Loh,Han Wei Hou,Jiong-Wei Wang,Minh Tn Le

Biomaterials science 10:6968-6979 PubMed36222485

2022

assessment of triazine lipid nanoparticles as transfection agents for plasmid DNA.

Applications

Unspecified application

Species

Unspecified reactive species

David Nardo,Michelle G Pitts,Rupinder Kaur,Vincent J Venditto

Frontiers in immunology 13:1057499 PubMed36389760

2022

An ambient-temperature stable nanoparticle-based vaccine for nasal application that confers long-lasting immunogenicity to carried antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Adolfo Cruz-Resendiz,Gonzalo Acero,Alicia Sampieri,Goar Gevorkian,Carolina Salvador,Laura Escobar,Margarita Jacaranda Rosendo-Pineda,Mara Medeiros,Luis Vaca

Acta pharmaceutica Sinica. B 13:2219-2233 PubMed35846427

2022

Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Fuhua Wu,Shuang Luo,Yongshun Zhang,Yangsen Ou,Hairui Wang,Zhaofei Guo,Chunting He,Shuting Bai,Penghui He,Min Jiang,Xiaoyan Chen,Guangsheng Du,Xun Sun

Nature communications 13:824 PubMed35149692

2022

Development of a skin- and neuro-attenuated live vaccine for varicella.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Dequan Pan,Wenkun Fu,Xiangzhong Ye,Jinle Han,Lianwei Yang,Jizong Jia,Jian Liu,Rui Zhu,Yali Zhang,Che Liu,Jianghui Ye,Anca Selariu,Yuqiong Que,Qinjian Zhao,Ting Wu,Yimin Li,Jun Zhang,Tong Cheng,Hua Zhu,Ningshao Xia

Marine drugs 18: PubMed33081290

2020

Viridicatol Isolated from Deep-Sea Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhendan Shu,Qingmei Liu,Cuiping Xing,Yafen Zhang,Yu Zhou,Jun Zhang,Hong Liu,Minjie Cao,Xianwen Yang,Guangming Liu

Plants (Basel, Switzerland) 9: PubMed32013187

2020

Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin.

Applications

Unspecified application

Species

Unspecified reactive species

Elena A Blokhina,Eugenia S Mardanova,Liudmila A Stepanova,Liudmila M Tsybalova,Nikolai V Ravin

View all publications

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Product promise

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Goat Anti-Mouse IgG2a heavy chain (HRP) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (13)

Product promise