JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB97248

Goat Anti-Mouse IgG2b heavy chain (Biotin)

Be the first to review this product! Submit a review

|

(2 Publications)

Suitable for ICC/IF, IHC-P, ELISA, WB. Cited in 2 publications.

View Alternative Names

IGHG2B, Ig gamma-2B chain C region, Igh-3, Immunoglobulin G2b, gamma2b, immunoglobulin heavy constant gamma 2B

  • 778 PE/Cy7®

    Goat Anti-Mouse IgG2b heavy chain (PE/Cy7®) preadsorbed

  • 785 APC/Cy7®

    Goat Anti-Mouse IgG2b heavy chain (APC/Cy7®) preadsorbed

  • Unconjugated

    Goat Anti-Mouse IgG2b heavy chain preadsorbed

  • HRP

    Goat Anti-Mouse IgG2b heavy chain (HRP)

  • 519 FITC

    Goat Anti-Mouse IgG2b heavy chain (FITC) preadsorbed

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG2b

Target specificity

Heavy chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

WB, ELISA, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with other immunoglobulins is less than 2%.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>ELISA-Primary</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>WB-Chemiluminescent</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

style
left-column

Target data

See full target information Mouse IgG2b
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12406-12418 PubMed33411367

2020

Immunological dynamics after subcutaneous immunization with a squalene-based oil-in-water adjuvant.

Applications

Unspecified application

Species

Unspecified reactive species

Sjoerd T T Schetters,Laura J W Kruijssen,Matheus H W Crommentuijn,Hakan Kalay,Joke M M den Haan,Yvette van Kooyk

Frontiers in immunology 9:990 PubMed29867967

2018

Mouse DC-SIGN/CD209a as Target for Antigen Delivery and Adaptive Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Sjoerd T T Schetters,Laura J W Kruijssen,Matheus H W Crommentuijn,Hakan Kalay,Jordi Ochando,Joke M M den Haan,Juan J Garcia-Vallejo,Yvette van Kooyk
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB172329

Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647)

Flow Cytometry - Rat monoclonal [SB76b] Anti-Mouse IgG3 gamma chain (Alexa Fluor® 647) (AB172329)

  • 0
  • 0
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB97255

Goat Anti-Mouse IgG2c heavy chain (HRP)

  • 0
  • 0
Secondary Antibodies

AB97245

Goat Anti-Mouse IgG2a heavy chain (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2a heavy chain (HRP) (AB97245)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com