AB97250

Goat Anti-Mouse IgG2b heavy chain (HRP)

Name: Goat Anti-Mouse IgG2b heavy chain (HRP) secondary antibody (ab97250) | Abcam
Brand: Abcam Limited
SKU: AB97250
Price: 265 USD
Availability: InStock

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(49 Publications)

Goat Anti-Mouse IgG2b heavy chain (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ELISA and ICC.

- Reacts specifically with mouse IgG2b
- Cross reactivity with other immunoglobulins is less than 2%
- Cited in over 30 publications

View Alternative Names

IGHG2B, Ig gamma-2B chain C region, Igh-3, Immunoglobulin G2b, gamma2b, immunoglobulin heavy constant gamma 2B

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (AB97250)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (AB97250)

IHC image of Coilin staining in human testis formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab87913, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97250, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG2b

Target specificity

Heavy chain

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, ELISA, ICC, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with other immunoglobulins is less than 2%.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"Similar dilution to primary antibody." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/25000", "notes":"WB-Colorimetric 1/2000 - 1/20000; WB-Chemiluminescent 1/5000 - 1/25000" } } }

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Product details

This antibody reacts with the heavy chain of Mouse IgG2b

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Mouse IgG2b

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Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1641109 PubMed40735325

2025

A novel adjuvant system BK-02 with CpG2006 and MF59 enhances the immunogenicity of a herpes zoster subunit vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Wang,Yingnan Guo,Xin Tang,Ying Sun,Tingting Wu,Hanyu Peng,Cenrong Wang,Weiheng Su,Chunlai Jiang,Yang Zang,Yaru Quan,Kangwei Xu,Bo Sun

iScience 28:112542 PubMed40487437

2025

Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Baba,Hiroki Hayashi,Shota Yoshida,Nanxiang Yin,Munehisa Shimamura,Ryuichi Morishita,Hiromi Rakugi,Hironori Nakagami,Koichi Yamamoto

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409837 PubMed40289872

2025

Microbiota-Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyu Gao,Yuhan Zhang,Zhi Hu,Shengwen Chen,Qiaolin Wang,Yong Zeng,Huiqi Yin,Junpeng Zhao,Yijing Zhan,Changxing Gao,Yue Xin,Bing Chen,Stijn van der Veen,Ming Zhao,Deyu Fang,Qianjin Lu

Cell transplantation 34:9636897251328419 PubMed40264358

2025

A novel method of pancreatic islet transplantation at the liver surface using a gelatin hydrogel nonwoven fabric.

Applications

Unspecified application

Species

Unspecified reactive species

Yukiko Endo Kumata,Akiko Inagaki,Yasuhiro Nakamura,Takehiro Imura,Ryusuke Saito,Takumi Katano,Shoki Suzuki,Kazuaki Tokodai,Takashi Kamei,Michiaki Unno,Kimiko Watanabe,Yasuhiko Tabata,Masafumi Goto

Molecular cancer 24:12 PubMed39806486

2025

Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Dafei Chai,Junhao Wang,Jing Ming Lim,Xiaohui Xie,Xinfang Yu,Dan Zhao,Perry Ayn Mayson Maza,Yifei Wang,Dana Cyril-Remirez,Ken H Young,Yong Li

Pathogens (Basel, Switzerland) 13: PubMed39770342

2025

An Evaluation of the Cellular and Humoral Response of a Multi-Epitope Vaccine Candidate Against COVID-19 with Different Alum Adjuvants.

Applications

Unspecified application

Species

Unspecified reactive species

Lineth Juliana Vega Rojas,Rocío Alejandra Ruíz-Manzano,Miguel Andrés Velasco-Elizondo,María Antonieta Carbajo-Mata,Diego Josimar Hernández-Silva,Mariana Rocha-Solache,Jesús Hernández,Rosa Martha Pérez-Serrano,Guadalupe Zaldívar-Lelo de Larrea,Teresa García-Gasca,Juan Mosqueda

ACS applied bio materials 7:7675-7683 PubMed39512153

2024

A Self-Amplifying Human Papillomavirus 16 Vaccine Candidate Delivered by Tobacco Mosaic Virus-Like Particles.

Applications

Unspecified application

Species

Unspecified reactive species

Sweta Karan,Jessica Fernanda Affonso De Oliveira,Miguel A Moreno-Gonzalez,Nicole F Steinmetz

Vaccines 12: PubMed39460288

2024

Sequential Immune Acquisition of Monoclonal Antibodies Enhances Phagocytosis of by Recognizing ATP Synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Huang,Zhujun Zeng,Zhuolin Li,Mengjun Li,Linlin Zhai,Yuhao Lin,Rui Xu,Jiuxin Qu,Bao Zhang,Wei Zhao,Chenguang Shen

Communications biology 7:1158 PubMed39284859

2024

An intranasal cationic liposomal polysaccharide vaccine elicits humoral immune responses against Streptococcus pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wei,Cecilia Romanò,Chengxin Li,Gael Clergeaud,Thomas L Andresen,Jonas R Henriksen,Anders E Hansen,Mads H Clausen

Journal of extracellular biology 2:e124 PubMed38938900

2024

Cancer cell invasion alters the protein profile of extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Jens C Luoto,Leila S Coelho-Rato,Cecilia Jungarå,Sara H Bengs,Jannica Roininen,John E Eriksson,Lea Sistonen,Eva Henriksson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG2b heavy chain (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (AB97250)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (49)

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