Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 36 publications.
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- ACS nano 18:3214-32332024Nanoparticle-Conjugated Toll-Like Receptor 9 Agonists Improve the Potency, Durability, and Breadth of COVID-19 Vaccines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBen S Ou,Julie Baillet,Vittoria C T M Picece,Emily C Gale,Abigail E Powell,Olivia M Saouaf,Jerry Yan,Anahita Nejatfard,Hector Lopez Hernandez,Eric A AppelPubMed 38215338
- iScience 26:1071202023Comparison of DNA vaccines with AS03 as an adjuvant and an mRNA vaccine against SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPraveen Neeli,Dafei Chai,Xu Wang,Navid Sobhani,George Udeani,Yong LiPubMed 37361876
- NPJ vaccines 8:882023A mosquito AgTRIO mRNA vaccine contributes to immunity against malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Min Chuang,Mohamad-Gabriel Alameh,Selma Abouneameh,Hamidah Raduwan,Michel Ledizet,Drew Weissman,Erol FikrigPubMed 37286568
- Journal of nanobiotechnology 21:1752023A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Xiang Jia,Sen-Lin Peng,Ndayambaje Yvan Kalisa,Qiang Chao,Zhifang Zhou,Xiao-Dong Gao,Ning WangPubMed 37264420
- Frontiers in microbiology 14:11174942023COVID-19 vaccines based on viral nanoparticles displaying a conserved B-cell epitope show potent immunogenicity and a long-lasting antibody response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Fernanda Affonso de Oliveira,Zhongchao Zhao,Yi Xiang,Matthew D Shin,Kathleen Elizabeth Villaseñor,Xinyi Deng,Sourabh Shukla,Shaochen Chen,Nicole F SteinmetzPubMed 37152732
- Nature communications 14:14622023B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiat Stoler-Barak,Ethan Harris,Ayelet Peres,Hadas Hezroni,Mirela Kuka,Pietro Di Lucia,Amalie Grenov,Neta Gurwicz,Meital Kupervaser,Bon Ham Yip,Matteo Iannacone,Gur Yaari,John D Crispino,Ziv ShulmanPubMed 36927854
- Small (Weinheim an der Bergstrasse, Germany) 19:e22058192022Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuxiong Chen,Diana H Quan,Gayathri Sam,Victoria Ozberk,Xiaonan T Wang,Peter Halfmann,Manisha Pandey,Michael F Good,Yoshihiro Kawaoka,Warwick J Britton,Bernd H A RehmPubMed 36564365
- Frontiers in immunology 13:10259312022B21 DNA vaccine expressing ag85b, rv2029c, and rv1738 confers a robust therapeutic effect against latent infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufeng Weng,Jinyi Zhang,Huixia Ma,Jingyu Zhou,Liqiu Jia,Yanmin Wan,Peng Cui,Qiaoling Ruan,Lingyun Shao,Jing Wu,Honghai Wang,Wenhong Zhang,Ying XuPubMed 36569899
- Advanced therapeutics 5:2022A single-dose Qβ VLP vaccine against S100A9 protein reduces atherosclerosis in a preclinical model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar A Ortega-Rivera,Matthew D Shin,Miguel A Moreno-Gonzalez,Jonathan K Pokorski,Nicole F SteinmetzPubMed 36570039
- Molecular therapy. Nucleic acids 28:743-7542022A taRNA vaccine candidate induces a specific immune response that protects mice against Chikungunya virus infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristin Schmidt,Erik Haefner,Julia Gerbeth,Tim Beissert,Ugur Sahin,Mario Perkovic,Barbara S SchnierlePubMed 35664702
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG2b
- Target specificity
- Heavy chain
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes Similar dilution to primary antibody. |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes WB-Colorimetric 1/2000 - 1/20000; WB-Chemiluminescent 1/5000 - 1/25000 |
Target data
Alternative names
IGHG2B, Ig gamma-2B chain C region, Igh-3, Immunoglobulin G2b, gamma2b, immunoglobulin heavy constant gamma 2B
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 36 publications.
Key facts
- Description
- Goat Anti-Mouse IgG2b heavy chain (HRP)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG2b
- Target specificity
- Heavy chain
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with other immunoglobulins is less than 2%.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)
IHC image of Coilin staining in human testis formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-Coilin antibody [IH10] ab87913, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97250, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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