AB97253
Goat Anti-Mouse IgG2c heavy chain (Biotin)
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(3 Publications)
Suitable for ICC/IF, IHC-P, ELISA, WB. Cited in 3 publications.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>(Primary ELISA)</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>(Chemiluminescent)</p>" } } }
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Target data
Publications (3)
Recent publications for all applications. Explore the full list and refine your search
The Journal of clinical investigation 132: PubMed36317628
2022
Stromal structure remodeling by B lymphocytes limits T cell activation in lymph nodes of Mycobacterium tuberculosis-infected mice.
Applications
Unspecified application
Species
Unspecified reactive species
Lina Daniel,Nayan D Bhattacharyya,Claudio Counoupas,Yi Cai,Xinchun Chen,James A Triccas,Warwick J Britton,Carl G Feng
NPJ vaccines 6:143 PubMed34848711
2021
A single dose, BCG-adjuvanted COVID-19 vaccine provides sterilising immunity against SARS-CoV-2 infection.
Applications
Unspecified application
Species
Unspecified reactive species
Claudio Counoupas,Matt D Johansen,Alberto O Stella,Duc H Nguyen,Angela L Ferguson,Anupriya Aggarwal,Nayan D Bhattacharyya,Alice Grey,Owen Hutchings,Karishma Patel,Rezwan Siddiquee,Erica L Stewart,Carl G Feng,Nicole G Hansbro,Umaimainthan Palendira,Megan C Steain,Bernadette M Saunders,Jason K K Low,Joel P Mackay,Anthony D Kelleher,Warwick J Britton,Stuart G Turville,Philip M Hansbro,James A Triccas
NPJ vaccines 5:105 PubMed33298977
2020
Mucosal delivery of a multistage subunit vaccine promotes development of lung-resident memory T cells and affords interleukin-17-dependent protection against pulmonary tuberculosis.
Applications
Unspecified application
Species
Unspecified reactive species
Claudio Counoupas,Kia C Ferrell,Anneliese Ashhurst,Nayan D Bhattacharyya,Gayathri Nagalingam,Erica L Stewart,Carl G Feng,Nikolai Petrovsky,Warwick J Britton,James A Triccas
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