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AB97253

Goat Anti-Mouse IgG2c heavy chain (Biotin)

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(3 Publications)

Suitable for ICC/IF, IHC-P, ELISA, WB. Cited in 3 publications.

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG2c

Target specificity

Heavy chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA, ICC/IF, IHC-P, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunogloublin serum proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>(Primary ELISA)</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>(Chemiluminescent)</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 132: PubMed36317628

2022

Stromal structure remodeling by B lymphocytes limits T cell activation in lymph nodes of Mycobacterium tuberculosis-infected mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Daniel,Nayan D Bhattacharyya,Claudio Counoupas,Yi Cai,Xinchun Chen,James A Triccas,Warwick J Britton,Carl G Feng

NPJ vaccines 6:143 PubMed34848711

2021

A single dose, BCG-adjuvanted COVID-19 vaccine provides sterilising immunity against SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Counoupas,Matt D Johansen,Alberto O Stella,Duc H Nguyen,Angela L Ferguson,Anupriya Aggarwal,Nayan D Bhattacharyya,Alice Grey,Owen Hutchings,Karishma Patel,Rezwan Siddiquee,Erica L Stewart,Carl G Feng,Nicole G Hansbro,Umaimainthan Palendira,Megan C Steain,Bernadette M Saunders,Jason K K Low,Joel P Mackay,Anthony D Kelleher,Warwick J Britton,Stuart G Turville,Philip M Hansbro,James A Triccas

NPJ vaccines 5:105 PubMed33298977

2020

Mucosal delivery of a multistage subunit vaccine promotes development of lung-resident memory T cells and affords interleukin-17-dependent protection against pulmonary tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Counoupas,Kia C Ferrell,Anneliese Ashhurst,Nayan D Bhattacharyya,Gayathri Nagalingam,Erica L Stewart,Carl G Feng,Nikolai Petrovsky,Warwick J Britton,James A Triccas
View all publications

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