AB97255

Goat Anti-Mouse IgG2c heavy chain (HRP)

Name: Goat Anti Mouse (IgG2c heavy chain) secondary antibody HRP (ab97255) | Abcam
Brand: Abcam Limited
SKU: AB97255

Be the first to review this product! Submit a review

(27 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 27 publications.

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG2c

Target specificity

Heavy chain

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, WB, ELISA, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunogloublin serum proteins.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(Primary ELISA)" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/25000", "notes":"(Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Mouse IgG2c

style

left-column

Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1641109 PubMed40735325

2025

A novel adjuvant system BK-02 with CpG2006 and MF59 enhances the immunogenicity of a herpes zoster subunit vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Wang,Yingnan Guo,Xin Tang,Ying Sun,Tingting Wu,Hanyu Peng,Cenrong Wang,Weiheng Su,Chunlai Jiang,Yang Zang,Yaru Quan,Kangwei Xu,Bo Sun

iScience 28:112542 PubMed40487437

2025

Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Baba,Hiroki Hayashi,Shota Yoshida,Nanxiang Yin,Munehisa Shimamura,Ryuichi Morishita,Hiromi Rakugi,Hironori Nakagami,Koichi Yamamoto

Science advances 11:eadr2631 PubMed40215318

2025

Generation of an inflammatory niche in a hydrogel depot through recruitment of key immune cells improves efficacy of mRNA vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Emily L Meany,John H Klich,Carolyn K Jons,Tianyang Mao,Namit Chaudhary,Ashley Utz,Julie Baillet,Ye E Song,Olivia M Saouaf,Ben S Ou,Shoshana C Williams,Noah Eckman,Darrell J Irvine,Eric Appel

Nature communications 16:2971 PubMed40140411

2025

An mRNA vaccine against monkeypox virus inhibits infection by co-activation of humoral and cellular immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Wanbo Tai,Chongyu Tian,Huicheng Shi,Benjie Chai,Xinyang Yu,Xinyu Zhuang,Pengyuan Dong,Min Li,Qi Yin,Shengyong Feng,Weixiao Wang,Oujia Zhang,Shibo Liang,Yang Liu,Jianying Liu,Longchao Zhu,Guangyu Zhao,Mingyao Tian,Guocan Yu,Gong Cheng

Journal of virology 99:e0232324 PubMed40035515

2025

Thymic stromal lymphopoietin improves protective immunity of the SARS-CoV-2 subunit vaccine by inducing dendritic cell-dependent germinal center response.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Hu,Ying Zhang,Housheng Zheng,Xiwen Zhao,Wei Ran,Chenghui Liao,Mingcheng Lu,Jian Zhou,Xun Song,Liang Ye

iScience 28:111568 PubMed39868033

2025

A subunit vaccine Ag85A-LpqH focusing on humoral immunity provides substantial protection against tuberculosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyuan Zeng,You Zuo,Minghui Tang,Chengrui Lei,Huoming Li,Xiuling Ma,Jiahong Ji,Hao Li

Communications biology 7:1158 PubMed39284859

2024

An intranasal cationic liposomal polysaccharide vaccine elicits humoral immune responses against Streptococcus pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wei,Cecilia Romanò,Chengxin Li,Gael Clergeaud,Thomas L Andresen,Jonas R Henriksen,Anders E Hansen,Mads H Clausen

NPJ vaccines 9:148 PubMed39143104

2024

Crimean Congo hemorrhagic fever virus nucleoprotein and GP38 subunit vaccine combination prevents morbidity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elif Karaaslan,Teresa E Sorvillo,Florine E M Scholte,Troy Justin O'Neal,Stephen R Welch,Katherine A Davies,JoAnn D Coleman-McCray,Jessica R Harmon,Jana M Ritter,Scott D Pegan,Joel M Montgomery,Jessica R Spengler,Christina F Spiropoulou,Éric Bergeron

Genes & diseases 11:101066 PubMed38550714

2024

Intranasal boosting with RBD-HR protein vaccine elicits robust mucosal and systemic immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Wenyan Ren,Hong Lei,Jiayu Wang,Haiying Que,Dandan Wan,Aqu Alu,Dandan Peng,Minyang Fu,Weiqi Hong,Yuhe Huang,Xiangrong Song,Guangwen Lu,Xiawei Wei

ACS nano 18:3214-3233 PubMed38215338

2024

Nanoparticle-Conjugated Toll-Like Receptor 9 Agonists Improve the Potency, Durability, and Breadth of COVID-19 Vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Ben S Ou,Julie Baillet,Vittoria C T M Picece,Emily C Gale,Abigail E Powell,Olivia M Saouaf,Jerry Yan,Anahita Nejatfard,Hector Lopez Hernandez,Eric A Appel

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG2c heavy chain (HRP)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (27)

Product promise