AB97260

Goat Anti-Mouse IgG3 heavy chain (HRP)

Name: Goat Anti Mouse (IgG3 heavy chain) secondary antibody HRP (ab97260) | Abcam
Brand: Abcam Limited
SKU: AB97260
Price: 220 USD
Availability: InStock

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(26 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 26 publications.

View Alternative Names

Ig gamma-3 chain C region

778 PE/Cy7®

Goat Anti-Mouse IgG3 gamma chain (PE/Cy7®) preadsorbed
Biotin

Goat Anti-Mouse IgG3 heavy chain (Biotin)
519 FITC

Goat Anti-Mouse IgG3 heavy chain (FITC)
Unconjugated

Goat Anti-Mouse IgG3 heavy chain preadsorbed
Alkaline Phosphatase

Goat Anti-Mouse IgG3 heavy chain (Alkaline Phosphatase) preadsorbed

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG3

Target specificity

Heavy chain

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, WB, ICC, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(as Primary)" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/25000", "notes":"Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-3 chain C region

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112542 PubMed40487437

2025

Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Baba,Hiroki Hayashi,Shota Yoshida,Nanxiang Yin,Munehisa Shimamura,Ryuichi Morishita,Hiromi Rakugi,Hironori Nakagami,Koichi Yamamoto

iScience 28:111568 PubMed39868033

2025

A subunit vaccine Ag85A-LpqH focusing on humoral immunity provides substantial protection against tuberculosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyuan Zeng,You Zuo,Minghui Tang,Chengrui Lei,Huoming Li,Xiuling Ma,Jiahong Ji,Hao Li

Vaccines 12: PubMed39460288

2024

Sequential Immune Acquisition of Monoclonal Antibodies Enhances Phagocytosis of by Recognizing ATP Synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Huang,Zhujun Zeng,Zhuolin Li,Mengjun Li,Linlin Zhai,Yuhao Lin,Rui Xu,Jiuxin Qu,Bao Zhang,Wei Zhao,Chenguang Shen

Communications biology 7:1158 PubMed39284859

2024

An intranasal cationic liposomal polysaccharide vaccine elicits humoral immune responses against Streptococcus pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wei,Cecilia Romanò,Chengxin Li,Gael Clergeaud,Thomas L Andresen,Jonas R Henriksen,Anders E Hansen,Mads H Clausen

Viruses 16: PubMed38543803

2024

Novel Universal Recombinant Rotavirus A Vaccine Candidate: Evaluation of Immunological Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitriy L Granovskiy,Nelli S Khudainazarova,Ekaterina A Evtushenko,Ekaterina M Ryabchevskaya,Olga A Kondakova,Marina V Arkhipenko,Marina V Kovrizhko,Elena P Kolpakova,Tatyana I Tverdokhlebova,Nikolai A Nikitin,Olga V Karpova

NPJ vaccines 8:88 PubMed37286568

2023

A mosquito AgTRIO mRNA vaccine contributes to immunity against malaria.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Min Chuang,Mohamad-Gabriel Alameh,Selma Abouneameh,Hamidah Raduwan,Michel Ledizet,Drew Weissman,Erol Fikrig

Journal of nanobiotechnology 21:175 PubMed37264420

2023

A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xiang Jia,Sen-Lin Peng,Ndayambaje Yvan Kalisa,Qiang Chao,Zhifang Zhou,Xiao-Dong Gao,Ning Wang

Small (Weinheim an der Bergstrasse, Germany) 19:e2205819 PubMed36564365

2022

Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiong Chen,Diana H Quan,Gayathri Sam,Victoria Ozberk,Xiaonan T Wang,Peter Halfmann,Manisha Pandey,Michael F Good,Yoshihiro Kawaoka,Warwick J Britton,Bernd H A Rehm

MedComm 3:e188 PubMed36474858

2022

Mixed formulation of mRNA and protein-based COVID-19 vaccines triggered superior neutralizing antibody responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jialu Zhang,Qian He,Xujia Yan,Jianyang Liu,Yu Bai,Chaoqiang An,Bopei Cui,Fan Gao,Qunying Mao,Junzhi Wang,Miao Xu,Zhenglun Liang

Frontiers in immunology 13:960405 PubMed36341456

2022

Antibody production in mice requires neither vitamin D, nor the vitamin D receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Lori A Plum,William Blaser,Logan Peter,Jean Prahl,Jeremy Seeman,Hector F DeLuca

View all publications

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Product promise

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Goat Anti-Mouse IgG3 heavy chain (HRP)

Related conjugates and formulations (5)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (26)

Product promise