AB47827

Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed

Name: Goat Anti-Mouse IgG+IgM (HRP) preadsorbed secondary antibody (ab47827) | Abcam
Brand: Abcam Limited
SKU: AB47827

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(34 Publications)

Suitable for EM, ICC, IHC-P, WB, ELISA, IHC-Fr. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 34 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-6, Ighm, Immunoglobulin heavy constant mu, Igh-4, Ighg1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (AB47827)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (AB47827)

ab47827 was used at dilution 1/500 with the primary antibody ab2869 in IHC-P. See the review on ab2869.

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG, IgM

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ICC, EM, IHC-P, ELISA, WB, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

Native Full Length Protein corresponding to Mouse Ighg1.

P01868

Specificity

No reaction was observed against bovine, horse or human serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/40000", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighm,Ighg1

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45:2189-2202 PubMed40569616

2025

Assessing the effects of aging on the Koizumi's and Zea Longa's methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyeon Lim,Hongrae Kim,Sehyun Lim,Hyo-Eun Kim,So-Jung Moon,Hyung-Hwan Kim,Suin Cho

Frontiers in pharmacology 16:1587351 PubMed40337519

2025

LL37 promotes angiogenesis: a potential therapeutic strategy for lower limb ischemic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Yang,Gang Wu,Yini Wang,Qiancheng Mao,Dongxu Zhang,Jitao Wu

Journal of neuroinflammation 21:261 PubMed39407211

2024

Glutamine metabolism modulates microglial NLRP3 inflammasome activity through mitophagy in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Zhang,Miao Li,Xiang Li,Zhiyang Feng,Gan Luo,Ying Wang,Xiaoyan Gao

Neural regeneration research 20:277-290 PubMed38767492

2024

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Cong,Jing-Jing Hu,Yan Yu,Xiao-Li Li,Xiao-Ting Sun,Li-Ting Wang,Xia Wu,Ling-Jie Zhu,Xiao-Jia Yang,Qian-Ru He,Fei Ding,Hai-Yan Shi

World journal of surgical oncology 22:128 PubMed38725005

2024

METTL14 regulates CD8T-cell activation and immune responses to anti-PD-1 therapy in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chongqi Sun,Jian Wang,Huixing Li,Luyao Liu,Yu Lin,Ling Zhang,Xianglong Zu,Yizhi Zhu,Yongqian Shu,Dong Shen,Qiong Wang,Yiqian Liu

Pharmaceutical biology 61:1512-1524 PubMed38069658

2023

Network pharmacology integrated with experimental verification reveals the antipyretic characteristics and mechanism of Zi Xue powder.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyu Zhang,Shining Ge,Fengyin Diao,Wen Song,Ying Zhang,Pengwei Zhuang,Yanjun Zhang

Aging 15:8113-8136 PubMed37595258

2023

Comprehensive characterization of pyroptosis phenotypes with distinct tumor immune profiles in gastric cancer to aid immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Kaida Huang,Yubiao Lin,Guoqin Qiu,Shengyu Wang,Lihua Feng,Zhigao Zheng,Yingqin Gao,Xin Fan,Wenhui Zheng,Jianmin Zhuang,Fanghong Luo,Shuitu Feng

Environmental toxicology 39:551-561 PubMed37436232

2023

Scoparone inhibits the development of hepatocellular carcinoma by modulating the p38 MAPK/Akt/NF-κB signaling in nonalcoholic fatty liver disease mice.

Applications

Unspecified application

Species

Unspecified reactive species

Miaoqing Ye,Chunyan Liu,Jiaojiao Liu,Fenping Lu,Jingdong Xue,Fenping Li,Yinghui Tang

Oncology letters 26:331 PubMed37415630

2023

Long non‑coding RNA PTCSC3 suppresses triple‑negative breast cancer by downregulating long non‑coding RNA MIR100HG.

Applications

Unspecified application

Species

Unspecified reactive species

Guojun Zhang,Lei Gao,Junliang Zhang,Rui Wang,Xiangdong Wei

PeerJ 11:e15441 PubMed37304872

2023

MFAP2 enhances cisplatin resistance in gastric cancer cells by regulating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Li,Hong-Yi Zhang,Rong-Gui Zhang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (AB47827)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (34)

Product promise