Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 42 publications.
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- Cell reports. Medicine 5:1014982024Oncostatin M signaling drives cancer-associated skeletal muscle wasting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAylin Domaniku-Waraich,Samet Agca,Batu Toledo,Melis Sucuoglu,Sevgi Döndü Özen,Sevval Nur Bilgic,Dilsad Hilal Arabaci,Aynur Erkin Kashgari,Serkan KirPubMed 38569555
- The Journal of physiology 601:4699-47212023Interleukin-1β triggers muscle-derived extracellular superoxide dismutase expression and protects muscles from doxorubicin-induced atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMami Yamada,Mitsuharu OkutsuPubMed 37815420
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e231562023Muscle p62 stimulates the expression of antioxidant proteins alleviating cancer cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMami Yamada,Eiji Warabi,Hisashi Oishi,Vitor A Lira,Mitsuharu OkutsuPubMed 37624620
- Biology 12:2023Neutrophil Extracellular Traps Correlate with Tumor Necrosis and Size in Human Malignant Melanoma Metastases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLennard Marten Weide,Fiona Schedel,Carsten WeishauptPubMed 37372107
- International journal of molecular sciences 24:2023Hamster Sperm Possess Functional Na/Ca-Exchanger 1: Its Implication in Hyperactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGen L Takei,Yuhei Ogura,Yoshihiro Ujihara,Fubito Toyama,Keitaro Hayashi,Tomoe FujitaPubMed 37240252
- Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 73:2023Assessment of mitochondrial respiratory capacity in relation to cardiac contractile performance in patients with normal to mildly decreased cardiac systolic function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Ljubkovic,F Runjic,C Bulat,M Cavar,L Frankovic,Z Marovic,N Bilopavlovic,J MarinovicPubMed 37087564
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229202023Glutamatergic melanocortin-4 receptor neurons regulate body weight.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaodong Liu,Xiaojing Li,Penghui Li,Rihan Hai,Jiacheng Li,Qi Fan,Xing Wang,Yujie Chen,Xiaojuan Cao,Xiaoyu Zhang,Ruifeng Gao,Kun Wang,Chenguang DuPubMed 37078546
- Life science alliance 6:2023Local tissue mechanics control cardiac pacemaker cell embryonic patterning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrevor Henley,Julie Goudy,Marietta Easterling,Carrie Donley,Robert Wirka,Michael BressanPubMed 36973005
- Frontiers in physiology 14:11274742023Delayed denervation-induced muscle atrophy in knockout mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingming Zhang,Ming Chen,Yi Li,Man Rao,Duanyang Wang,Zhongqi Wang,Licheng Zhang,Pengbin Yin,Peifu TangPubMed 36909232
- Viruses 15:2022Influence of Heparan Sulfate Proteoglycans and Factor X on species D Human Adenovirus Uptake and Transduction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatrin Schröer,Montaha Alshawabkeh,Sebastian Schellhorn,Katrin Bronder,Wenli Zhang,Anja EhrhardtPubMed 36680095
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgM
- Target specificity
- Mu chain
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (By similarity). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (By similarity). Isoform 1. Constant region of secreted IgM (sIgM), also known as the Fc region of IgM antibody. Able to multimerize, forms high order polymers, mainly pentamers and occasionally hexamers, providing for multivalency and high avidity recognition of antigens (By similarity). Natural sIgM are polyreactive and recognize conserved self- and pathogen-derived structures, whereas immune sIgM are secreted only upon exposure to pathogens and are antigen-specific. Both natural and immune sIgM are required for an efficient humoral immune response to infection (PubMed:10899913, PubMed:28135254, PubMed:34788614). Mediates sIgM effector functions mostly via Fc receptors and the complement system. On lymphoid cells binds high-affinity Fc receptor FCMR and promotes induction of an efficient neutralizing IgG response while maintaining tolerance to self-antigens. Recruits C1q complement component to initiate the classical complement pathway, facilitating the recognition and neutralization of pathogens by the host. Together with C1q and mannose-binding lectin promotes the phagocytosis of apoptotic cells by macrophages, ensuring the clearance of potential autoimmune epitopes from tissues (By similarity) (PubMed:10899913, PubMed:11062505, PubMed:28135254). Involved in mucosal immunity. It is transported by transcytosis across mucosal epithelium by PIGR and secreted on the apical side in complex with PIGR secretory component to scan mucosal lining for pathogens. IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across mucosal M cells toward antigen-presenting cells in mucosal lymphoid tissues (PubMed:34788614). Isoform 2. Constant region of membrane-bound IgM, part of the B cell receptor complex (BCR). IgM BCR provides constitutive tonic signaling for B cell survival. Mediates pre-BCR signaling that regulates B cell selection and rearrangement of Ig genes via allelic exclusion.
Alternative names
Igh-6, Ighm, Immunoglobulin heavy constant mu
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 42 publications.
Key facts
- Description
- Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgM
- Target specificity
- Mu chain
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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5 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
ICC/IF image of Anti-Vimentin antibody [VI-10] ab20346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with Anti-Vimentin antibody [VI-10] ab20346 at 1/500 dilution. The secondary antibody (green) was ab150121 used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
Anti-Vimentin antibody [LN-6] ab230171 staining Vimentin in HeLa-VIM cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Vimentin antibody [LN-6] ab230171 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046. Cells were then incubated with ab150121 at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
Anti-A2B5 antibody [105] ab53521 staining A2B5 in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-A2B5 antibody [105] ab53521 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046. Cells were then incubated with ab150121 at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121)
Anti-A2B5 antibody [105] ab53521 staining A2B5 in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-A2B5 antibody [105] ab53521 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046. Cells were then incubated with ab150121 at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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