Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 15 publications.
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- Stem cell research & therapy 14:2052023NG2-positive pericytes regulate homeostatic maintenance of slow-type skeletal muscle with rapid myonuclear turnover.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakamitsu Tatsukawa,Kohei Kano,Kei-Ichi Nakajima,Takashi Yazawa,Ryoji Eguchi,Maki Kabara,Kiwamu Horiuchi,Taiki Hayasaka,Risa Matsuo,Naoyuki Hasebe,Nobuyoshi Azuma,Jun-Ichi KawabePubMed 37592340
- Alcohol, clinical & experimental research 47:1271-12822023Impact of prior alcohol use on the subsequent development of cancer cachexia in male and female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph A Laudato,Abigail L Tice,Bonde R Johnson,Allyson Russo,Michael L Rossetti,Blake O Bridges,Arthur Egan,Bradley S Gordon,Jennifer L SteinerPubMed 37208964
- eLife 12:2023Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Li,Xinqi Lou,Yingjie Zha,Yinyin Qin,Jun Zha,Lei Hong,Zhanli Xie,Shudi Yang,Chen Wang,Jianzhong An,Zhenhao Zhang,Shigang QiaoPubMed 37010266
- The Journal of cell biology 222:2023Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Shi Liu,Yicheng Wang,Xiaoman Zhou,Linpei Zhang,Ganglong Yang,Xiao-Dong Gao,Yoshiko Murakami,Morihisa Fujita,Taroh KinoshitaPubMed 36828365
- Signal transduction and targeted therapy 8:282023Proximity-enabled covalent binding of IL-2 to IL-2Rα selectively activates regulatory T cells and suppresses autoimmunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Zhang,Jiaqi Sun,Yeshuang Yuan,Dezhong Ji,Yeting Sun,Yudong Liu,Shengjie Li,Xingxing Zhu,Xunyao Wu,Jin Hu,Qiu Xie,Ling Wu,Lulu Liu,Boyang Cheng,Yuanjie Zhang,Lingjuan Jiang,Lidan Zhao,Fei Yu,Wei Song,Min Wang,Yue Xu,Shiliang Ma,Yunyun Fei,Lihe Zhang,Demin Zhou,Xuan ZhangPubMed 36690610
- Drug development research 83:1858-18662022Neuroprotective effects of nerolidol against Alzheimer's disease in Wistar rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeyman Taheri,Parichehreh Yaghmaei,Zahra Hajebrahimi,Kazem ParivarPubMed 36321205
- Advanced healthcare materials 11:e22004482022One-Step Bioprinting of Multi-Channel Hydrogel Filaments Using Chaotic Advection: Fabrication of Pre-Vascularized Muscle-Like Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdna Johana Bolívar-Monsalve,Carlos Fernando Ceballos-González,Carolina Chávez-Madero,Brenda Guadalupe de la Cruz-Rivas,Silvana Velásquez Marín,Shirley Mora-Godínez,Luisa María Reyes-Cortés,Ali Khademhosseini,Paul S Weiss,Mohamadmahdi Samandari,Ali Tamayol,Mario Moisés Alvarez,Grissel Trujillo-de SantiagoPubMed 35930168
- Research (Washington, D.C.) 2022:97942352022Helper T Cell (CD4) Targeted Tacrolimus Delivery Mediates Precise Suppression of Allogeneic Humoral Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Shen,Chang Liu,Pengpeng Yan,Meifang Wang,Luying Guo,Shuaihui Liu,Jianghua Chen,Jessica M Rosenholm,Hongfeng Huang,Rending Wang,Hongbo ZhangPubMed 35958106
- Nature immunology 22:1107-11172021Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengchong Li,Mengdi Jiang,Ketian Li,Hao Li,Yangzhong Zhou,Xinyue Xiao,Yue Xu,Suzanne Krishfield,Peter E Lipsky,George C Tsokos,Xuan ZhangPubMed 34385713
- Cell death discovery 7:1802021Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengchan Li,Kunmin Yan,Lili Wu,Zhong Zheng,Yun Du,Ziting Liu,Luyao Zhao,Wei Li,Yulan Sheng,Lijie Ren,Chaojun Tang,Li ZhuPubMed 34282126
Key facts
- Host species
Goat
- Target species
Mouse
- Target isotype
IgM
- Target specificity
Mu chain
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Target data
Function
IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions (By similarity).
Alternative names
Immunoglobulin heavy constant mu, Igh-6, Ighm
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 15 publications.
Key facts
- Description
Goat Anti-Mouse IgM mu chain (Alexa Fluor® 647)
- Host species
Goat
- Target species
Mouse
- Target isotype
IgM
- Target specificity
Mu chain
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
Polyclonal
- Pre-adsorbed
No
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 647) (ab150123)
ICC/IF image of Anti-Vimentin antibody [VI-10] ab20346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Vimentin antibody [VI-10] ab20346, 1/500) overnight at +4°C. The secondary antibody (red) was ab150123 used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 647) (ab150123)
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