Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 51 publications.
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- Frontiers in immunology 15:13769072024Suppression of the growth and metastasis of mouse melanoma by and tapeworms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManfred Schreiber,Tomáš Macháček,Vojtěch Vajs,Barbora Šmídová,Martin Majer,Jiří Hrdý,Ondřej Tolde,Jan Brábek,Daniel Rösel,Petr HorákPubMed 38571957
- Science advances 9:eadg71122023FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYajie Liu,Kairan Yu,Xiaotian Kong,Keren Zhang,Lingyan Wang,Nana Zhang,Qiushi Chen,Mingshan Niu,Wenli Li,Xiaomin Zhong,Sijin Wu,Jianing Zhang,Yubo LiuPubMed 37595040
- EMBO reports 24:e564582023O-GlcNAcylation promotes topoisomerase IIα catalytic activity in breast cancer chemoresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangzhi Liu,Kairan Yu,Keren Zhang,Mingshan Niu,Qiushi Chen,Yajie Liu,Lingyan Wang,Nana Zhang,Wenli Li,Xiaomin Zhong,Guohui Li,Sijin Wu,Jianing Zhang,Yubo LiuPubMed 37249035
- Nature immunology 24:1124-11372023Spatial dysregulation of T follicular helper cells impairs vaccine responses in aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyssa Silva-Cayetano,Sigrid Fra-Bido,Philippe A Robert,Silvia Innocentin,Alice R Burton,Emily M Watson,Jia Le Lee,Louise M C Webb,William S Foster,Ross C J McKenzie,Alexandre Bignon,Ine Vanderleyden,Dominik Alterauge,Julia P Lemos,Edward J Carr,Danika L Hill,Isabella Cinti,Karl Balabanian,Dirk Baumjohann,Marion Espeli,Michael Meyer-Hermann,Alice E Denton,Michelle A LintermanPubMed 37217705
- NPJ vaccines 8:722023A candidate subunit vaccine induces protective immunity against Mycobacterium avium subspecies paratuberculosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingzhu Shao,Ning Cui,Yangyang Tang,Fanruo Chen,Yingying Cui,Guanghui Dang,Siguo LiuPubMed 37210376
- Frontiers in microbiology 14:11174942023COVID-19 vaccines based on viral nanoparticles displaying a conserved B-cell epitope show potent immunogenicity and a long-lasting antibody response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Fernanda Affonso de Oliveira,Zhongchao Zhao,Yi Xiang,Matthew D Shin,Kathleen Elizabeth Villaseñor,Xinyi Deng,Sourabh Shukla,Shaochen Chen,Nicole F SteinmetzPubMed 37152732
- Nature communications 14:14622023B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiat Stoler-Barak,Ethan Harris,Ayelet Peres,Hadas Hezroni,Mirela Kuka,Pietro Di Lucia,Amalie Grenov,Neta Gurwicz,Meital Kupervaser,Bon Ham Yip,Matteo Iannacone,Gur Yaari,John D Crispino,Ziv ShulmanPubMed 36927854
- Frontiers in immunology 13:9604052022Antibody production in mice requires neither vitamin D, nor the vitamin D receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLori A Plum,William Blaser,Logan Peter,Jean Prahl,Jeremy Seeman,Hector F DeLucaPubMed 36341456
- Biological & pharmaceutical bulletin 45:1133-11412022A Chinese Medicine, Tokishakuyakusan, Increases Bovine Oviductal Tonus via G Protein-Coupled Estrogen Receptor 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSayaka Kubota,Yuki Yamamoto,Koji KimuraPubMed 35908895
- Advanced therapeutics 5:2022A single-dose Qβ VLP vaccine against S100A9 protein reduces atherosclerosis in a preclinical model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar A Ortega-Rivera,Matthew D Shin,Miguel A Moreno-Gonzalez,Jonathan K Pokorski,Nicole F SteinmetzPubMed 36570039
Key facts
- Host species
Goat
- Target species
Mouse
- Target isotype
IgM
- Target specificity
Mu chain
- Conjugation
HRP
- Applications
ICC, IHC-P, ELISA, WB
- Clonality
Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary ELISA) |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000) |
Target data
Function
IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions (By similarity).
Alternative names
Immunoglobulin heavy constant mu, Igh-6, Ighm
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 51 publications.
Alternative names
Immunoglobulin heavy constant mu, Igh-6, Ighm
Key facts
- Description
Goat Anti-Mouse IgM mu chain (HRP)
- Host species
Goat
- Target species
Mouse
- Target isotype
IgM
- Target specificity
Mu chain
- Conjugation
HRP
- Applications
ICC, IHC-P, ELISA, WB
- Clonality
Polyclonal
- Pre-adsorbed
No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Product promise
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgM mu chain (HRP) (ab97230)
IHC image of Hsp70 staining in human lung formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-Hsp70 antibody [2A4] ab5442, 1/2000 dilution overnight at +4°C. An HRP-conjugated secondary (ab97230, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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