Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 12 publications.
Images
Publications
Filter by
- Translational psychiatry 13:3322023Chronic administration of XBD173 ameliorates cognitive deficits and neuropathology via 18 kDa translocator protein (TSPO) in a mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpit Kumar Pradhan,Tatjana Neumüller,Claudia Klug,Severin Fuchs,Martin Schlegel,Markus Ballmann,Katharina Johanna Tartler,Antoine Pianos,Maria Sanchez Garcia,Philippe Liere,Michael Schumacher,Matthias Kreuzer,Rainer Rupprecht,Gerhard RammesPubMed 37891168
- Communications biology 6:8462023Sexual dimorphism in the social behaviour of Cntnap2-null mice correlates with disrupted synaptic connectivity and increased microglial activity in the anterior cingulate cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatt S Dawson,Kevin Gordon-Fleet,Lingxin Yan,Vera Tardos,Huanying He,Kwong Mui,Smriti Nawani,Zeinab Asgarian,Marco Catani,Cathy Fernandes,Uwe DrescherPubMed 37582968
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:597-6142023Bone Deterioration in Response to Chronic High-Altitude Hypoxia Is Attenuated by a Pulsed Electromagnetic Field Via the Primary Cilium/HIF-1α Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxia Hao,Dan Wang,Zedong Yan,Yuanjun Ding,Juan Zhang,Juan Liu,Xi Shao,Xiyu Liu,Lu Wang,Erping Luo,Jing Cai,Da JingPubMed 36680558
- STAR protocols 3:1016872022Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpit Kumar Pradhan,Qinfang Shi,Katharina Johanna Tartler,Gerhard RammesPubMed 36125933
- International journal of molecular sciences 23:2022Role of Periostin Expression in Non-Small Cell Lung Cancer: Periostin Silencing Inhibits the Migration and Invasion of Lung Cancer Cells via Regulation of MMP-2 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Ratajczak-Wielgomas,Alicja Kmiecik,Piotr DziegielPubMed 35163164
- Journal of diabetes investigation 13:22-332021VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi Wen,Fei Deng,Lulu Li,Li Xu,Xin Li,Qiuling FanPubMed 34494385
- Nature microbiology 6:769-7822021Dinoflagellate symbionts escape vomocytosis by host cell immune suppression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie R Jacobovitz,Sebastian Rupp,Philipp A Voss,Ira Maegele,Sebastian G Gornik,Annika GusePubMed 33927382
- Oncogenesis 9:182020PERK-mediated expression of peptidylglycine α-amidating monooxygenase supports angiogenesis in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHimanshu Soni et. al.PubMed 32054826
- Cell reports 28:3395-3405.e62019Structural Definition of a Neutralization-Sensitive Epitope on the MERS-CoV S1-NTD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNianshuang Wang,Osnat Rosen,Lingshu Wang,Hannah L Turner,Laura J Stevens,Kizzmekia S Corbett,Charles A Bowman,Jesper Pallesen,Wei Shi,Yi Zhang,Kwanyee Leung,Robert N Kirchdoerfer,Michelle M Becker,Mark R Denison,James D Chappell,Andrew B Ward,Barney S Graham,Jason S McLellanPubMed 31553909
- eLife 8:2019Sterol transfer by atypical cholesterol-binding NPC2 proteins in coral-algal symbiosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Ann Hambleton et. al.PubMed 31159921
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Fc region
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 12 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488)
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Fc region
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (ab150089)
ICC/IF image of Anti-beta Actin antibody - Loading Control ab8227 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Actin antibody - Loading Control ab8227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150089 Alexa Fluor® 488 goat anti-rabbit IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (ab150089)
Emission spectra of the Alexa Fluor® conjugated secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (ab150089)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling SARS-CoV-2 orf3d with primary antibody anti-SARS-CoV-2 orf3d (Anti-SARS-CoV-2 orf3d antibody [EPR25045-51] ab284040) at 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor® 594) (ab150089) (green) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a SARS-CoV2 orf3d expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-CoV2 orf8 expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain cells at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com