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AB150089

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488)

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(14 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 14 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (AB150089)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (AB150089)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling SARS-CoV-2 orf3d with primary antibody anti-SARS-CoV-2 orf3d (ab284040) at 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor® 594) (ab150089) (green) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a SARS-CoV2 orf3d expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-CoV2 orf8 expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain cells at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (AB150089)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (AB150089)

ICC/IF image of ab8227 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150089 Alexa Fluor® 488 goat anti-rabbit IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (AB150089)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) (AB150089)

Emission spectra of the Alexa Fluor® conjugated secondary antibodies.

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-Fr, Flow Cyt, IHC-P, ELISA, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Rabbit IgG
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 5:100965 PubMed39826552

2025

A thermoplastic chip for 2D and 3D correlative assays combining screening and high-resolution imaging of immune cell responses.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna van Ooijen,Quentin Verron,Hanqing Zhang,Patrick A Sandoz,Thomas W Frisk,Valentina Carannante,Karl Olofsson,Arnika K Wagner,Niklas Sandström,Björn Önfelt

Bioengineering (Basel, Switzerland) 11: PubMed39451336

2024

Galectin-3/Gelatin Electrospun Scaffolds Modulate Collagen Synthesis in Skin Healing but Do Not Improve Wound Closure Kinetics.

Applications

Unspecified application

Species

Unspecified reactive species

Karrington A McLeod,Madeleine Di Gregorio,Dylan Tinney,Justin Carmichael,David Zuanazzi,Walter L Siqueira,Amin Rizkalla,Douglas W Hamilton

Translational psychiatry 13:332 PubMed37891168

2023

Chronic administration of XBD173 ameliorates cognitive deficits and neuropathology via 18 kDa translocator protein (TSPO) in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Arpit Kumar Pradhan,Tatjana Neumüller,Claudia Klug,Severin Fuchs,Martin Schlegel,Markus Ballmann,Katharina Johanna Tartler,Antoine Pianos,Maria Sanchez Garcia,Philippe Liere,Michael Schumacher,Matthias Kreuzer,Rainer Rupprecht,Gerhard Rammes

Communications biology 6:846 PubMed37582968

2023

Sexual dimorphism in the social behaviour of Cntnap2-null mice correlates with disrupted synaptic connectivity and increased microglial activity in the anterior cingulate cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Matt S Dawson,Kevin Gordon-Fleet,Lingxin Yan,Vera Tardos,Huanying He,Kwong Mui,Smriti Nawani,Zeinab Asgarian,Marco Catani,Cathy Fernandes,Uwe Drescher

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:597-614 PubMed36680558

2023

Bone Deterioration in Response to Chronic High-Altitude Hypoxia Is Attenuated by a Pulsed Electromagnetic Field Via the Primary Cilium/HIF-1α Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxia Hao,Dan Wang,Zedong Yan,Yuanjun Ding,Juan Zhang,Juan Liu,Xi Shao,Xiyu Liu,Lu Wang,Erping Luo,Jing Cai,Da Jing

STAR protocols 3:101687 PubMed36125933

2022

Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q.

Applications

Unspecified application

Species

Unspecified reactive species

Arpit Kumar Pradhan,Qinfang Shi,Katharina Johanna Tartler,Gerhard Rammes

International journal of molecular sciences 23: PubMed35163164

2022

Role of Periostin Expression in Non-Small Cell Lung Cancer: Periostin Silencing Inhibits the Migration and Invasion of Lung Cancer Cells via Regulation of MMP-2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Ratajczak-Wielgomas,Alicja Kmiecik,Piotr Dziegiel

Journal of diabetes investigation 13:22-33 PubMed34494385

2021

VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Si Wen,Fei Deng,Lulu Li,Li Xu,Xin Li,Qiuling Fan

Nature microbiology 6:769-782 PubMed33927382

2021

Dinoflagellate symbionts escape vomocytosis by host cell immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Marie R Jacobovitz,Sebastian Rupp,Philipp A Voss,Ira Maegele,Sebastian G Gornik,Annika Guse

Oncogenesis 9:18 PubMed32054826

2020

PERK-mediated expression of peptidylglycine α-amidating monooxygenase supports angiogenesis in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Himanshu Soni,Julia Bode,Chi D L Nguyen,Laura Puccio,Michelle Neßling,Rosario M Piro,Jonas Bub,Emma Phillips,Robert Ahrends,Betty A Eipper,Björn Tews,Violaine Goidts
View all publications
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