Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed

• ICC

Lab

Immunocytochemistry - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling eIF4A3 with ab180573 at 1/90 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SD Rat primary neuron cells labelling Pan Trk with ab267830 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG Fc (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized ICR Mouse primary neuron cells labelling Pan Trk with ab267830 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG Fc (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (green) was ab150091 Alexa Fluor® 488 goat anti-rabbit IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150091 Alexa Fluor® 488 goat anti-rabbit IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (AB150097)