JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB150090

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555)

Be the first to review this product! Submit a review

|

(4 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.
3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) (AB150090)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) (AB150090)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150090 Alexa Fluor® 555 goat anti-rabbit IgG Fc used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) (AB150090)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) (AB150090)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150090 Alexa Fluor® 555 goat anti-rabbit IgG Fc used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) (AB150090)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) (AB150090)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

style
left-column

Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
style
left-column

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

style
left-column

Target data

See full target information Rabbit IgG
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular neurobiology 45:78 PubMed40788579

2025

Role of Repressive Histone Lysine Demethylases and Methylases in Susceptibility to Depression Using a Novel Progressive Social Defeat Stress Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Arpan Mukhoti,P K Annapoorna,Ashutosh Kumar,Pratishtha Wadnerkar,Ayesha Atqa Khan,Salil Saurav Pathak,Sumana Chakravarty,Arvind Kumar

Cancers 14: PubMed36428580

2022

Effect of Collagen Matrix on Doxorubicin Distribution and Cancer Cells' Response to Treatment in 3D Tumor Model.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Druzhkova,Elena Nikonova,Nadezhda Ignatova,Irina Koryakina,Mikhail Zyuzin,Artem Mozherov,Dmitriy Kozlov,Dmitry Krylov,Daria Kuznetsova,Uliyana Lisitsa,Vladislav Shcheslavskiy,Evgeny A Shirshin,Elena Zagaynova,Marina Shirmanova

Nature communications 11:246 PubMed31937764

2020

Synaptic density marker SV2A is reduced in schizophrenia patients and unaffected by antipsychotics in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ellis Chika Onwordi,Els F Halff,Thomas Whitehurst,Ayla Mansur,Marie-Caroline Cotel,Lisa Wells,Hannah Creeney,David Bonsall,Maria Rogdaki,Ekaterina Shatalina,Tiago Reis Marques,Eugenii A Rabiner,Roger N Gunn,Sridhar Natesan,Anthony C Vernon,Oliver D Howes

Frontiers in immunology 9:516 PubMed29616030

2018

Recognition of Double-Stranded RNA and Regulation of Interferon Pathway by Toll-Like Receptor 10.

Applications

Unspecified application

Species

Unspecified reactive species

Suki Man-Yan Lee,Tsz-Fung Yip,Sheng Yan,Dong-Yan Jin,Hong-Li Wei,Rey-Ting Guo,Joseph Sriyal Malik Peiris
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com