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AB150098

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed

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(1 Publication)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed (AB150098)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed (AB150098)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150098, goat polyclonal secondary antibody to Rabbit IgG - FC (Alexa Fluor®; 555), pre-adsorbed, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed (AB150098)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed (AB150098)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150098, goat polyclonal secondary antibody to Rabbit IgG - FC (Alexa Fluor® 555), pre-adsorbed, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed (AB150098)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555) preadsorbed (AB150098)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, Flow Cyt, IHC-P, IHC-Fr, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Rabbit IgG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxicology in vitro : an international journal pub 37:88-96 PubMed27622579

2016

Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells.

Applications

Unspecified application

Species

Unspecified reactive species

Delyan P Ivanov,Abdal-Jabbar Al-Rubai,Anna M Grabowska,Margaret K Pratten
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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