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AB150092

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594)

5

(1 Review)

|

(18 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 18 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594) (AB150092)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594) (AB150092)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150092 Alexa Fluor® 594 goat anti-rabbit IgG Fc used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594) (AB150092)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594) (AB150092)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150092 Alexa Fluor® 594 goat anti-rabbit IgG Fc used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594) (AB150092)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594) (AB150092)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

IHC-P, Flow Cyt, ICC/IF, ELISA, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Rabbit IgG
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1422 PubMed41044405

2025

Estradiol and dihydrotestosterone exert sex-specific effects on human fibroblast and endothelial proliferation, bioenergetics, and vasculogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley T Martier,Yasmin V Maurice,K Michael Conrad,Delia A Carlino,Franck Mauvais-Jarvis,Mark J Mondrinos

Journal of nanobiotechnology 23:445 PubMed40524151

2025

Biosynthesized nanoparticles of Tibetan medicine mercuric sulfide preparation to promote endocytosis and realize drug crossing through blood brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Jia,Huilan Yue,Liying Liu,Luya Wang,Lin Zhang,Jihong Tao,Guoying Zhou,Lixin Wei,Hongxin Dong,Rinchen Dhondrup,Xiaohui Zhao

Journal of immunology research 2024:5556042 PubMed39534554

2024

Manual Therapy Exerts Local Anti-Inflammatory Effects Through Neutrophil Clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwen Liu,Shiguo Yuan,Kai Zheng,Gaofeng Liu,Junhua Li,Baofei Ye,Yangkun Wang,Li Yin,Yikai Li

Frontiers in immunology 13:854445 PubMed35479088

2022

Identification of Anti-Collapsin Response Mediator Protein 2 Antibodies in Patients With Encephalitis or Encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaibiao Xu,Dongmei Wang,Yan He,Shengnan Wang,Guanghui Liu,Yue Pan,Haishan Jiang,Yu Peng,Fenliang Xiao,Yihua Huang,Qiqi Wang,Yongming Wu,Suyue Pan,Yafang Hu

iScience 24:103225 PubMed34712922

2021

IL-17A reprograms intestinal epithelial cells to facilitate HIV-1 replication and outgrowth in CD4+ T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Raul Wiche Salinas,Annie Gosselin,Laurence Raymond Marchand,Etiene Moreira Gabriel,Olivier Tastet,Jean-Philippe Goulet,Yuwei Zhang,Dragos Vlad,Hanane Touil,Jean-Pierre Routy,Mariana G Bego,Mohamed El-Far,Nicolas Chomont,Alan L Landay,Éric A Cohen,Cécile Tremblay,Petronela Ancuta

International journal of molecular sciences 21: PubMed33120955

2020

β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Filipa Amaro,Dany Silva,Henrique Reguengo,José C Oliveira,Clara Quintas,Nuno Vale,Jorge Gonçalves,Paula Fresco

Frontiers in physiology 10:1248 PubMed31611818

2019

Syndecans and Enzymes for Heparan Sulfate Biosynthesis and Modification Differentially Correlate With Presence of Inflammatory Infiltrate in Periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Roko Duplancic,Marija Roguljic,Ivan Puhar,Nika Vecek,Ruzica Dragun,Katarina Vukojevic,Mirna Saraga-Babic,Darko Kero

Molecular reproduction and development 86:1663-1670 PubMed31410930

2019

BRCA1 regulates HMGA2 levels in the Swan71 trophoblast cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel C West,Jennifer E Russ,Gerrit J Bouma,Quinton A Winger

Molecular reproduction and development 86:1086-1093 PubMed31215730

2019

LIN28B regulates androgen receptor in human trophoblast cells through Let-7c.

Applications

Unspecified application

Species

Unspecified reactive species

Erin S McWhorter,Rachel C West,Jennifer E Russ,Asghar Ali,Quinton A Winger,Gerrit J Bouma

The EMBO journal 37: PubMed30158112

2018

Function of HNRNPC in breast cancer cells by controlling the dsRNA-induced interferon response.

Applications

Unspecified application

Species

Unspecified reactive species

Yusheng Wu,Wenwei Zhao,Yang Liu,Xiangtian Tan,Xin Li,Qin Zou,Zhengtao Xiao,Hui Xu,Yuting Wang,Xuerui Yang
View all publications
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