AB97200

Goat Anti-Rabbit IgG Fc (HRP)

Name: Goat Anti Rabbit (IgG Fc) secondary antibody HRP (ab97200) | Abcam
Brand: Abcam Limited
SKU: AB97200
Price: 265 USD
Availability: InStock

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(72 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 72 publications.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (AB97200)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (AB97200)

IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Unknown

Western blot - Goat Anti-Rabbit IgG Fc (HRP) (AB97200)

ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

false

673 DyLight® 650

Goat Anti-Rabbit IgG Fc (DyLight® 650) preadsorbed
519 FITC

Goat Anti-Rabbit IgG Fc (FITC) preadsorbed
Biotin

Goat Anti-Rabbit IgG Fc (Biotin)
518 DyLight® 488

Goat Anti-Rabbit IgG Fc (DyLight® 488)
603 Alexa Fluor® 568

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 568)
519 Alexa Fluor® 488

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488)
565 Alexa Fluor® 555

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 555)
665 Alexa Fluor® 647

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 647)
775 Alexa Fluor® 750

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 750)
617 Alexa Fluor® 594

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 594)
Unconjugated

Goat Anti-Rabbit IgG Fc preadsorbed

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, ELISA, WB, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis, this antibody reacts specifically with Rabbit IgG-Fc Fragment. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(Primary ELISA)" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/25000", "notes":"(Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (72)

Recent publications for all applications. Explore the full list and refine your search

BMC biotechnology 25:72 PubMed40670938

2025

An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking.

Applications

Unspecified application

Species

Unspecified reactive species

Manman Lin,Guangjie Han,Chuanming Li,Lixin Huang,Qin Liu,Nan Zhang,Yang Xia,Yurong Lu,Xianjin Liu,Yuan Liu,Jian Xu,Johnson Lin

Cirugia y cirujanos 93:151-157 PubMed40199318

2025

Evaluation of the effectiveness of human breast milk exosomes in experimental testicular torsion-detorsion injury model.

Applications

Unspecified application

Species

Unspecified reactive species

Ünal T Öztürk,Hatice S Y Cömert,Gül Şalcı,Ahmet Alver,Sevdegül A Mungan,Neslihan Sağlam,Şeniz Erdem,Serdar Karakullukçu,Mustafa İmamoğlu,Haluk Sarıhan

The Journal of biological chemistry 301:108067 PubMed39667502

2024

Iron regulatory protein 1-deficient mice exhibit hypospermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Aileen Harrer,Niraj Ghatpande,Tiziana Grimaldini,Daniela Fietz,Vishnu Kumar,Christiane Pleuger,Monika Fijak,Dankward T Föppl,Lennart P Rynio,Hans-Christian Schuppe,Adrian Pilatz,Marek Bartkuhn,Tara Procida-Kowalski,Noga Guttmann-Raviv,Sudhanshu Bhushan,Esther G Meyron-Holtz,Andreas Meinhardt

Pathology, research and practice 256:155233 PubMed38452583

2024

SIRT7: A potential prognostic marker and therapeutic target in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Xu,Xiaojing Cai,Guohao Cai,Guoyu Huang

JCI insight 9: PubMed38301068

2024

Iron regulatory proteins 1 and 2 have opposing roles in regulating inflammation in bacterial orchitis.

Applications

Unspecified application

Species

Unspecified reactive species

Niraj Ghatpande,Aileen Harrer,Bar Azoulay-Botzer,Noga Guttmann-Raviv,Sudhanshu Bhushan,Andreas Meinhardt,Esther G Meyron-Holtz

Andrology 12:1389-1397 PubMed38148671

2023

Testosterone replacement therapy: association with mortality in high-risk patient subgroups.

Applications

Unspecified application

Species

Unspecified reactive species

Amar Mann,Richard C Strange,Carola S König,Geoffrey Hackett,Ahmad Haider,Karim Sultan Haider,Peter Desnerck,Sudarshan Ramachandran

Frontiers in oncology 13:1178021 PubMed37483514

2023

Cystathionine β-synthase affects organization of cytoskeleton and modulates carcinogenesis in colorectal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Liskova,Barbora Chovancova,Petr Babula,Ingeborg Rezuchova,Kristina Ploth Pavlov,Miroslava Matuskova,Olga Krizanova

Cell division 18:9 PubMed37301821

2023

SIRT4 is an independent prognostic factor in bladder cancer and inhibits bladder cancer growth by suppressing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yin,Guohao Cai,Huaiwen Wang,Weijia Chen,Shan Liu,Guoyu Huang

Physiological reports 10:e15474 PubMed36325585

2022

Muscarinic receptors in the rat ovary are involved in follicular development but not in steroid secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda C Cuevas,Daniela Bastias,Constanza Alanis,Agustin Benitez,Valentina Squicciarini,Raul Riquelme,Pia Sessenhausen,Artur Mayerhofer,Hernan E Lara

iScience 25:104811 PubMed35982794

2022

Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Cabral,Jessica P Otis,Kimberly L Mowry

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Rabbit IgG Fc (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (AB97200)

Western blot - Goat Anti-Rabbit IgG Fc (HRP) (AB97200)

Related conjugates and formulations (11)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (72)

Product promise