Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 66 publications.
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- Frontiers in oncology 13:11780212023Cystathionine β-synthase affects organization of cytoskeleton and modulates carcinogenesis in colorectal carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronika Liskova,Barbora Chovancova,Petr Babula,Ingeborg Rezuchova,Kristina Ploth Pavlov,Miroslava Matuskova,Olga KrizanovaPubMed 37483514
- Cell division 18:92023SIRT4 is an independent prognostic factor in bladder cancer and inhibits bladder cancer growth by suppressing autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yin,Guohao Cai,Huaiwen Wang,Weijia Chen,Shan Liu,Guoyu HuangPubMed 37301821
- Physiological reports 10:e154742022Muscarinic receptors in the rat ovary are involved in follicular development but not in steroid secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernanda C Cuevas,Daniela Bastias,Constanza Alanis,Agustin Benitez,Valentina Squicciarini,Raul Riquelme,Pia Sessenhausen,Artur Mayerhofer,Hernan E LaraPubMed 36325585
- iScience 25:1048112022Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah E Cabral,Jessica P Otis,Kimberly L MowryPubMed 35982794
- Scientific reports 12:122082022SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuohao Cai,Zhuhui Ge,Yunqiu Xu,Liangliang Cai,Pingliang Sun,Guoyu HuangPubMed 35842463
- FEBS open bio 12:913-9242022Camptothecin, triptolide, and apoptosis inducer kit have differential effects on mitochondria in colorectal carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronika Liskova,Marek Kajsik,Barbora Chovancova,Ladislav Roller,Olga KrizanovaPubMed 35318813
- Frontiers in endocrinology 12:7575922021Sperm Proteome Analysis to Investigate DNA Repair Mechanisms in Varicocele Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenata Finelli,Sara Darbandi,Peter Natesan Pushparaj,Ralf Henkel,Edmund Ko,Ashok AgarwalPubMed 34975746
- BMC cancer 21:10852021Knockdown of circ_0011946 targets miR-216a-5p/BCL2L2 axis to regulate proliferation, migration, invasion and apoptosis of oral squamous cell carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhou,Shuhong Zhang,Zhonghan Min,Zhongwei Yu,Huaiwei Zhang,Jiao JiaoPubMed 34620126
- Nanomaterials (Basel, Switzerland) 11:2021Optical Monitoring of the Biodegradation of Porous and Solid Silicon Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaxim B Gongalsky,Nikolay V Pervushin,Daria E Maksutova,Uliana A Tsurikova,Pavel P Putintsev,Oleg D Gyuppenen,Yana V Evstratova,Olga A Shalygina,Gelina S Kopeina,Andrey A Kudryavtsev,Boris Zhivotovsky,Liubov A OsminkinaPubMed 34578485
- Experimental and therapeutic medicine 22:8462021Upregulated osteoprotegerin expression promotes lung cancer cell invasion by increasing miR-20a expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Wan,Ziwei Tu,Zhentian Liu,Yun Cai,Yinglan Chen,Chunhua LingPubMed 34149892
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Fc region
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary ELISA) |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000) |
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 66 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG Fc (HRP)
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Fc region
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis, this antibody reacts specifically with Rabbit IgG-Fc Fragment. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)
IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-beta Actin antibody - Loading Control ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)
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