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AB175652

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

5

(2 Reviews)

|

(36 Publications)

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) is a secondary antibody with a maximum absorption wavelength of 401nm and a maximum emission wavelength of 421nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

4 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)

ICC/IF image of ab6046 stained HeLa cells. The cells were 4% PFA fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 5μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)
  • ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)

Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

This data was developed using the unconjugated antibody (ab182016).

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)
  • ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)

Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

This data was developed using the unconjugated antibody (ab182016).

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (AB175652)

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ICC/IF, Flow Cyt, IHC-P, IHC-Fr, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" } } }
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You may be interested in:

AB175660

Goat Anti-Mouse IgG H&L (Alexa Fluor® 405)

5

1 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 11: PubMed38140228

2023

Lipid A Is a Potent Adjuvant That Induces Sterilizing Immunity against Leptospirosis.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek P Varma,Mohammad Kadivella,Sridhar Kavela,Syed M Faisal

American journal of cancer research 13:5577-5589 PubMed38058835

2023

Detection of CSTF2 by nano fluorescent probe and its correlation with malignant biological characteristics in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongmin Jiang,Xiaozhi Liu,Yongqing Deng, Duojierenqing, Renqingcuo,Wenhan Wu,Wei Meng,Zhijiang Shao

Communications biology 6:749 PubMed37468760

2023

Identifying dysregulated immune cell subsets following volumetric muscle loss with pseudo-time trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren A Hymel,Shannon E Anderson,Thomas C Turner,William Y York,Hongmanlin Zhang,Adrian R Liversage,Hong Seo Lim,Peng Qiu,Luke J Mortensen,Young C Jang,Nick J Willett,Edward A Botchwey

Cells 12: PubMed37408275

2023

Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Viktória Pevná,Georges Wagnières,Daniel Jancura,Veronika Huntošová

International journal of molecular sciences 24: PubMed37240373

2023

Parkin Precipitates on Mitochondria via Aggregation and Autoubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa T Ardah,Nada Radwan,Engila Khan,Tohru Kitada,M Emdadul Haque

Nature communications 14:1820 PubMed37002243

2023

Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Hua Wu,Yang-Yang He,Zhang-Rong Chen,Ze-Yuan He,Yi Yan,Yangzhige He,Guang-Ming Wang,Yu Dong,Ying Yang,Yi-Min Sun,Yong-Hong Ren,Qiu-Yan Zhao,Xiao-Dan Yang,Li-Ying Wang,Cai-Jun Fu,Miao He,Si-Jin Zhang,Ji-Fen Fu,Hong Liu,Zhi-Cheng Jing

The Journal of physiology 601:1407-1424 PubMed36869605

2023

Antagonism of TRPV4 channels partially reduces mechanotransduction in rat skeletal muscle afferents.

Applications

Unspecified application

Species

Unspecified reactive species

Ayumi Fukazawa,Amane Hori,Norio Hotta,Kimiaki Katanosaka,Juan A Estrada,Rie Ishizawa,Han-Kyul Kim,Gary A Iwamoto,Scott A Smith,Wanpen Vongpatanasin,Masaki Mizuno

Frontiers in physiology 14:1127474 PubMed36909232

2023

Delayed denervation-induced muscle atrophy in knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Zhang,Ming Chen,Yi Li,Man Rao,Duanyang Wang,Zhongqi Wang,Licheng Zhang,Pengbin Yin,Peifu Tang

Cell & bioscience 13:37 PubMed36810259

2023

Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xia,Yang Li,Wanting Xu,Chengwei Wu,Hanfei Zheng,Liqun Liu,Lei Dong

eLife 12: PubMed36656755

2023

Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Theodore J Zwang,Rachel E Bennett,Maria Lysandrou,Benjamin Woost,Anqi Zhang,Charles M Lieber,Douglas S Richardson,Bradley T Hyman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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