AB175654

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

Name: Goat Anti-Rabbit IgG (Alexa Fluor® 405) preadsorbed secondary antibody (ab175654) | Abcam
Brand: Abcam Limited
SKU: AB175654
Price: 275 USD
Availability: InStock

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(9 Publications)

Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175654 Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L, pre-adsorbed, used at 1μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

Flow Cyt, ELISA, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:2201-2216 PubMed41044342

2025

Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Ionescu,Lior Ankol,Anand Ganapathy Subramaniam,Topaz Altman,Iddo Magen,Yahel Cohen,Yehuda Danino,Tal Gradus-Pery,Yoav Niv,Ori Bar Avi,Danielle Geller,Amjd Ibraheem,Ruilei Cheng,Noam Steinberg,Leenor Alfahel,Pauline Duc,Zeynep Ergul-Ulger,Doruk Arslan,Ersin Tan,Florence Rage,Nilo Riva,Angello Quattrini,Can Ebru Bekircan-Kurt,Adrian Israelson,Amir Dori,Eran Hornstein,Eran Perlson

Science advances 9:eadd6480 PubMed37595032

2023

Organoid cell fate dynamics in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Zheng,Max A Betjes,Pascal Ender,Yvonne J Goos,Guizela Huelsz-Prince,Hans Clevers,Jeroen S van Zon,Sander J Tans

Molecular neurobiology 60:1766-1781 PubMed36571720

2022

Activation of CREB-BDNF Pathway in Pyramidal Neurons in the Hippocampus Improves the Neurological Outcome of Mice with Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Jiang,Qingying Liu,Yumei Zhao,Chunyang Wang,Ming Sun

Glia 70:106-122 PubMed34498776

2021

Increasing astrogenesis in the developing hippocampus induces autistic-like behavior in mice via enhancing inhibitory synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Chen,Xiao-Lin Ma,Hui Zhao,Xiao-Yu Wang,Min-Xin Xu,Hua Wang,Tian-Qi Yang,Cheng Peng,Shuang-Shuang Liu,Man Huang,Yu-Dong Zhou,Yi Shen

Molecular cell 79:934-949.e14 PubMed32822587

2020

Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls Adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Huang,Cristel V Camacho,Rohit Setlem,Keun Woo Ryu,Balaji Parameswaran,Rana K Gupta,W Lee Kraus

Reproductive biology and endocrinology : RB&E 17:41 PubMed31060561

2019

Analyses of bovine luteal fractions obtained by FACS reveals enrichment of miR-183-96-182 cluster miRNAs in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bushra T Mohammed,Cristina L Esteves,F Xavier Donadeu

Translational psychiatry 7:e1192 PubMed28786974

2017

Brain antibodies in the cortex and blood of people with schizophrenia and controls.

Applications

IHC-Fr

Species

Human

L J Glass,D Sinclair,D Boerrigter,K Naude,S J Fung,D Brown,V S Catts,P Tooney,M O'Donnell,R Lenroot,C Galletly,D Liu,T W Weickert,C Shannon Weickert

Stem cells and development 26:964-972 PubMed28376684

2017

Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.

Applications

Flow Cyt

Species

Horse

Cristina L Esteves,Tara A Sheldrake,Lucy Dawson,Timothy Menghini,Burgunde Elisabeth Rink,Karin Amilon,Nusrat Khan,Bruno Péault,Francesc Xavier Donadeu

Stem cell research & therapy 8:80 PubMed28420427

2017

Isolation and characterization of equine native MSC populations.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina L Esteves,Tara A Sheldrake,Simone P Mesquita,Juan J Pesántez,Timothy Menghini,Lucy Dawson,Bruno Péault,F Xavier Donadeu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise