Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.
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- Nature communications 12:45512021Disruption of NIPBL/Scc2 in Cornelia de Lange Syndrome provokes cohesin genome-wide redistribution with an impact in the transcriptome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Garcia,Rita Fernandez-Hernandez,Ana Cuadrado,Ignacio Coca,Antonio Gomez,Maria Maqueda,Ana Latorre-Pellicer,Beatriz Puisac,Feliciano J Ramos,Juan Sandoval,Manel Esteller,Jose Luis Mosquera,Jairo Rodriguez,J Pié,Ana Losada,Ethel QueraltPubMed 34315879
- Biomicrofluidics 14:0441022020Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasatomo Chikamori et. al.PubMed 32665806
- Molecular reproduction and development 86:1053-10662019Thymoquinone reduces intracytoplasmic oxidative stress and improves epigenetic modification in polycystic ovary syndrome mice oocytes, during in-vitro maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Eini,Arash Bidadkosh,Hamid Nazarian,Abbas Piryaei,Marefat Ghaffari Novin,Khojasteh JoharchiPubMed 31209968
- Cell and tissue research 376:123-1352018Solubilized eggshell membrane supplies a type III collagen-rich elastic dermal papilla.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEri Ohto-Fujita,Miho Shimizu,Shoei Sano,Masashi Kurimoto,Kai Yamazawa,Tomoaki Atomi,Takashi Sakurai,Yoshihiko Murakami,Taku Takami,Tomoaki Murakami,Kotaro Yoshimura,Yukio Hasebe,Yoriko AtomiPubMed 30448901
- Animal science journal = Nihon chikusan Gakkaiho 90:49-542018Significance of CCN2 expression in bovine preimplantation development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Akizawa,Yojiro Yanagawa,Masashi Nagano,Hanako Bai,Masashi Takahashi,Manabu KawaharaPubMed 30358017
- Urology journal 15:116-1212018Evaluation of PAWP and PLC? Expression in Infertile Men with Previous ICSI Fertilization Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNahid Azad et. al.PubMed 29397567
- Reproduction (Cambridge, England) 155:563-5712018Reciprocal regulation of TEAD4 and CCN2 for the trophectoderm development of the bovine blastocyst.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Akizawa et. al.PubMed 29661794
- Physiological reports 4:2016Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization.Applications:IHCReactive species:Unspecified reactive speciesAlex J Pasternak,Glenn M Hamonic,Andrew Van Kessel,Heather L WilsonPubMed 27821716
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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8 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150082 Alexa Fluor® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150082 Alexa Fluor® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image is courtesy of Dr. Shaohua LiImmunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse embryonic fibroblasts
Preparation:
Fix in 3% PFA in PBS for 30 min at RT
Primary antibody: Rabbit anti-integrin beta3 (ab75872), 1:100
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 555) pre-adsorbed (ab150082), 1:200
FITC-phalloidin, 1:100
Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Pan Cav2 with Anti-Pan Cav2 antibody [EPR28073-84] ab315092 at 1/50 (10.32 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: confocal image showing no staining on mouse liver. The section was incubated with Anti-Pan Cav2 antibody [EPR28073-84] ab315092 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CSF-1-R with Anti-CSF-1-R antibody [EPR29702-379] ab319160 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing membranous with cytoplasmic staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression: NIH/3T3 (PMID: 36555673).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling ER81/ETV1 with Anti-ER81/ETV1 antibody [EPR28393-132] ab314874 at 1/50 (10.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing nuclear staining in PC-3 cells.
Negative control: MCF7(PMID:27604655).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labeling CD90 / Thy1 with Anti-CD90 / Thy1 antibody [EPR28145-53] ab307736 at 1/250 dilution (1.8 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing membranous and cytoplasmic staining in EL4 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
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