AB175471

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

Name: Goat anti-rabbit IgG (Alexa Fluor® 568) secondary antibody (ab175471) | Abcam
Brand: Abcam Limited
SKU: AB175471
Price: 130 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(197 Publications)

Goat anti-rabbit IgG H&L (Alexa Fluor® 568) is a secondary antibody with a maximum absorption wavelength of 578nm and a maximum emission wavelength of 603nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 190 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

4 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% Methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175471 Alexa Fluor^® 568 goat anti-rabbit IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

This data was developed using the unconjugated antibody (ab182016).

ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

This data was developed using the unconjugated antibody (ab182016).

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

Beta-galactosidase

Goat Anti-Rabbit IgG H&L (beta-galactosidase)
Glucose Oxidase

Goat Anti-Rabbit IgG H&L (Glucose Oxidase)
HRP polymer

Goat Anti-Rabbit IgG H&L (HRP polymer)
Unconjugated

Goat Anti-Rabbit IgG H&L preadsorbed
Alkaline Phosphatase

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
667 Cy5®

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
505 Cy2®

Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
519 FITC

Goat Anti-Rabbit IgG H&L (FITC)
572 TRITC

Goat Anti-Rabbit IgG H&L (TRITC)
6nm Gold

Goat Anti-Rabbit IgG H&L (6nm Gold)
618 DyLight® 594

Goat Anti-Rabbit IgG H&L (DyLight® 594)
576 DyLight® 550

Goat Anti-Rabbit IgG H&L (DyLight® 550)
673 DyLight® 650

Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
702 Alexa Fluor® 680

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)
519 Alexa Fluor® 488

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
565 Alexa Fluor® 555

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)
665 Alexa Fluor® 647

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)
617 Alexa Fluor® 594

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)
702 Alexa Fluor® 680

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed
Biotin

Goat Anti-Rabbit IgG H&L (Biotin)
HRP

Goat Anti-Rabbit IgG H&L (HRP)
775 Alexa Fluor® 750

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed
421 Alexa Fluor® 405

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)
578 PE

Goat Anti-Rabbit IgG H&L (PE) preadsorbed
615 Texas Red®

Goat Anti-Rabbit IgG H&L (Texas Red ®)
660 APC

Goat Anti-Rabbit IgG H&L (APC) preadsorbed
518 DyLight® 488

Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
703 Cy5.5®

Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed
565 Cy3®

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
596 Cy3.5®

Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed
805 Alexa Fluor® 790

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" } } }

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You may be interested in:

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

15 Reviews

View product

Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (197)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:43 PubMed38217571

2024

Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Tithi Mandal,Arikta Biswas,Tanmoy Ghosh,Sreekanth Manikandan,Avijit Kundu,Ayan Banerjee,Dhrubaditya Mitra,Bidisha Sinha

Frontiers in cell and developmental biology 11:1253274 PubMed38020889

2023

INO80 function is required for mouse mammary gland development, but mutation alone may be insufficient for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen Xuan Thang,Dong Wook Han,Chanhyeok Park,Hyeonji Lee,Hyeonwoo La,Seonho Yoo,Heeji Lee,Sang Jun Uhm,Hyuk Song,Jeong Tae Do,Kyoung Sik Park,Youngsok Choi,Kwonho Hong

Frontiers in immunology 14:1259521 PubMed37954611

2023

Tuft cells utilize taste signaling molecules to respond to the pathobiont microbe in the proximal colon.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Lei,Defu Yu,Yan-Bo Xue,Yi-Hong Li,Shi-Meng Gong,Yuan-Yuan Peng,Kai-Fang Liu,Damiano Buratto,Yisen Yang,Sai-Sai Zhang,Min Wu,Ruhong Zhou,Liquan Huang

PloS one 18:e0291029 PubMed37751459

2023

Functional characterization of a single nucleotide polymorphism associated with Alzheimer's disease in a hiPSC-based neuron model.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay R Stolzenburg,Sahar Esmaeeli,Ameya S Kulkarni,Erin Murphy,Taekyung Kwon,Christina Preiss,Lamiaa Bahnassawy,Joshua D Stender,Justine D Manos,Peter Reinhardt,Fedik Rahimov,Jeffrey F Waring,Cyril Y Ramathal

Frontiers in cellular neuroscience 17:1254923 PubMed37771931

2023

Replenished microglia partially rescue schizophrenia-related stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Yan,Fang-Ling Xuan,Song Chen,Mengzhuang Gou,Wenjin Chen,Yanli Li,Zhiren Wang,Leilei Wang,Ting Xie,Fengmei Fan,Alexander Zharkovsky,Yunlong Tan,Li Tian

Science advances 9:eade2120 PubMed37585527

2023

Fibroblasts generate topographical cues that steer cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Baschieri,Abigail Illand,Jorge Barbazan,Olivier Zajac,Clémence Henon,Damarys Loew,Florent Dingli,Danijela Matic Vignjevic,Sandrine Lévêque-Fort,Guillaume Montagnac

Brain communications 5:fcad218 PubMed37601407

2023

Neuron-binding antibody responses are associated with Black ethnicity in multiple sclerosis during natalizumab treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Kiel M Telesford,Chad Smith,Marcel Mettlen,Melissa B Davis,Lindsay Cowell,Rick Kittles,Timothy Vartanian,Nancy Monson

Frontiers in microbiology 14:1212242 PubMed37637123

2023

Interferon-stimulated gene 15 facilitates BTV replication through interacting with the NS1 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Di Kang,Guorui Zhang,Zhonghui Zhang,Zhancheng Tian,Shandian Gao,Guangyuan Liu,Guiquan Guan,Jianxun Luo,Hong Yin,Junzheng Du

BMC medicine 21:286 PubMed37542262

2023

CSF1R regulates schizophrenia-related stress response and vascular association of microglia/macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Yan,Yanli Li,Fengmei Fan,Mengzhuang Gou,Fangling Xuan,Wei Feng,Keerthana Chithanathan,Wei Li,Junchao Huang,Hongna Li,Wenjin Chen,Baopeng Tian,Zhiren Wang,Shuping Tan,Alexander Zharkovsky,L Elliot Hong,Yunlong Tan,Li Tian

Nature communications 14:4591 PubMed37524699

2023

The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Djakovic,Thomas Hennig,Katharina Reinisch,Andrea Milić,Adam W Whisnant,Katharina Wolf,Elena Weiß,Tobias Haas,Arnhild Grothey,Christopher S Jürges,Michael Kluge,Elmar Wolf,Florian Erhard,Caroline C Friedel,Lars Dölken

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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goat-rabbit-igg-h-l-alexa-fluor-790-ab175781

(0 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

Related conjugates and formulations (31)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

You may be interested in:

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Why is this recommended?

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (197)

Product promise

Associated Products

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L (HRP polymer)

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Secondary Antibodies

Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2, pre-adsorbed

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L preadsorbed

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L (FITC) preadsorbed

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L (HRP)

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L (Biotin)

Alternative Product

Secondary Antibodies

Goat F(ab) Anti-Rabbit IgG H&L (FITC)

Alternative Product

Secondary Antibodies

Goat F(ab) Anti-Rabbit IgG H&L (TRITC)

Alternative Product

Secondary Antibodies

Goat F(ab) Anti-Rabbit IgG H&L (Texas Red ®)

Alternative Product

Secondary Antibodies

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

Alternative Product

Secondary Antibodies

Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed

Alternative Product

Secondary Antibodies

Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin)