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Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 68 publications.

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Images

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt

Clonality

Polyclonal

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Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/200.00000 - 1/1000.00000

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/2000

Notes

-

Alternative names

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Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 68 publications.

Alternative names

Key facts

Description

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt

Clonality

Polyclonal

Pre-adsorbed

Yes

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

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15 product images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    ab8245 staining GAPDH in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab8245 at 5 μg/ml and ab6046 at 1 μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 2 μg/ml (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    ab7751 staining beta III Tubulin in Neuro-2a cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab7751 at 1/1000 and ab6046 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Rabbit secondary (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1- Rabbit primary and anti-mouse secondary antibody; 2 - Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    ab7291 staining alpha Tubulin in NIH3T3 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab7291 at 1μl/ml and ab6046 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green) and anti-rabbit AlexaFluor® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1- Rabbit primary antibody and anti-mouse secondary antibody; 2 - Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    ab7291 staining alpha-Tubulin in Caco-2 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab7291 at 1μg/ml and ab6046 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green) and anti-rabbit AlexaFluor® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150088 Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150088 Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling mNeonGreen with ab321887 at 1/2000 (0.249 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a mNeonGreen-GPGPG-SP1 expression vector (shown in green). ab321887 is shown in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling GFP with ab314656 at 1/50 (10.18 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining in 293T cells transfected with a empty expression vector containing a GFP tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2 ug/mL) dilution.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    ab109028 staining HP1 alpha in HeLa (human cervical adenocarcinoma epithelial cell). The cells were fixed with 100% methanol, permeabilized with 0.1% TritonX-100. The cells were then incubated with ab109028 at 1:100 dilution. Cells were then incubated with ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1:1000 dilution (shown in green). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as counterstain at 1:200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Confocal image showing nuclear staining in HeLa cell line.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) cells labelling SARS-CoV-2 orf7a with primary antibody anti-SARS-CoV-2 orf7a (ab283962) at 1/100 dilution, followed by AlexaFluor®594 Goat anti-Rabbit (green) (ab150088) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a SARS-CoV-2 orf7a and a SARS-CoV-1 orf7a expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-CoV-2 orf8 expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).
    Secondary antibody only control : Secondary antibody is ab150088 AlexaFluor®594 Goat anti-Rabbit (green) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Noradrenaline with ab315969 at 1/50 (10.06 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 (2ug/ml) dilution (Magenta).

    Confocal image showing positive staining in RAW 264.7 cells treated with 0.5 mg/mL Norepinephrine-crosslink-BSA-FITC for 4 hours and no staining when 0.5 mg/mL crosslink-BSA-FITC was incubated for 4 hours.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).



    The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2ug/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling GPCR GPR17 with ab316105 at 1/50 (10.18 ug/ml) dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2 ug/mL) dilution (Green).

    Panel A: merged staining of anti-GPCR GPR17 (ab316105, green), anti-Olig2 (ab225099, red) and anti-GFAP (ab313596, grey) on mouse cerebellum.

    Panel B: anti-GPCR GPR17 stained on mouse cerebellum.

    Panel C: anti-Olig2 stained in oligodendrocyte of mouse cerebellum.

    Panel D: anti-GFAP stained in astrocytes of mouse cerebellum.

    The nuclear counterstain was DAPI (Blue). The section was incubated with ab316105 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling GPCR GPR17 with ab316105 at 1/50 (10.18 ug/ml) dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2 ug/mL) dilution (Green).

    Panel A: merged staining of anti-GPCR GPR17 (ab316105, green), anti-Olig2 (ab225099, red) and anti-GFAP (ab313596, grey) on rat cerebellum.

    Panel B: anti-GPCR GPR17 stained on rat cerebellum.

    Panel C: anti-Olig2 stained in oligodendrocyte of rat cerebellum.

    Panel D: anti-GFAP stained in astrocytes of rat cerebellum.

    The nuclear counterstain was DAPI (Blue). The section was incubated with ab316105 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)at 1/1000 (2 ug/mL) dilution.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling SARS-CoV2 orf8 with primary antibody anti-SARS-CoV2 orf8 (ab283914) at 1/100 dilution, followed by AlexaFluor®594 Goat anti-Rabbit (green) (ab150088) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with a SARS-CoV2 orf8 expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-COV2 orf7a expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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