Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

31 product images

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  GAPDH immunofluorescence staining of HeLa cells using mouse anti-GAPDH antibody
  
  Anti-GAPDH antibody [6C5] - Loading Control ab8245 staining GAPDH in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

  The cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with Anti-GAPDH antibody [6C5] - Loading Control ab8245 at 5 μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046 at 1 μg/ml overnight at +4°C followed by a further incubation at room temperature for 1 hour with Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

  Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker ab7751 staining beta III Tubulin in Neuro-2a cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker ab7751 at 1/1000 and Anti-beta Tubulin antibody - Loading Control ab6046 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor^®488 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and AlexaFluor^®594 Goat anti-Rabbit secondary (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
  Negative controls: 1- Rabbit primary and anti-mouse secondary antibody; 2 - Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 staining alpha Tubulin in NIH3T3 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1μl/ml and Anti-beta Tubulin antibody - Loading Control ab6046 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor^® 488 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and anti-rabbit AlexaFluor^® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
  Negative controls: 1- Rabbit primary antibody and anti-mouse secondary antibody; 2 - Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 staining alpha-Tubulin in Caco-2 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor^® 488 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and anti-rabbit AlexaFluor^® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
  

  Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150088 Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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  Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)
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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150088 Alexa Fluor® 594 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] ab317837 at 1/500 (2.062 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 16 L1 or HPV 18 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  A: Myc transfected cells
  
  B: HPV 11 L1-Myc transfected cells
  
  C: HPV 18 L1-Myc transfected cells
  
  D: HPV 16 L1-Myc transfected cells
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] ab317837 at 1/500 (2.062 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 6B L1 or HPV13 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  A: Myc transfected cells
  
  B: HPV 11 L1-Myc transfected cells
  
  C: HPV 6B L1-Myc transfected cells
  
  D: HPV13 L1-Myc transfected cells
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/100 (5 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus L2 protein with Anti-Human papillomavirus L2 antibody [MK18L2_K3] ab322523 at 1/1000 (1.04 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 18 L2 protein, a Human papillomavirus type 16 L2 protein, a Human papillomavirus type 31 L2 protein, a Human papillomavirus type 45 L2 protein, a Human papillomavirus type 51 L2 protein, a Human papillomavirus type 6B L2 protein and a Human papillomavirus type 11 L2 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis C Virus region NS5B with Anti-Hepatitis C Virus region NS5B antibody [1A4] ab322521 at 1/2000 (0.47 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) region NS5B expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human Papilloma Virus type 35 L1 protein with Anti-Human Papilloma Virus type 35 L1 antibody [LH35L1_A] ab322518 at 1/100 (10.66 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing nuclear staining in 293T cells transfected with a HPV 35 L1 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a HPV 16 L1 or HPV 11 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  A: Myc transfected cells
  
  B: HPV 35 L1-Myc transfected cells
  
  C: HPV 16 L1-Myc transfected cells
  
  D: HPV 11 L1-Myc transfected cells
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2ug/ml) dilution (Magenta).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [4.15] ab322235 at 1/2000 (0.56 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells transfected with a Human respiratory syncytial virus subtype B1 Fusion (F) Glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus subtype A2 Fusion (F) Glycoprotein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 16 L1 protein with Anti-Human Papilloma Virus type 16 L1 antibody [16L1_VIII_1.3.5.15] ab321901 at 1/2000 (0.57 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1000 2ug/ml dilution (Green).

  Confocal image showing nuclear staining in 293T cells transfected with a HPV 16 L1 protein expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a HPV 18 L1 protein or HPV 6B L1 protein or HPV 11 L1 protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [11-6-F9] ab322234 at 1/2000 (0.48 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cells transfected with a Human respiratory syncytial virus subtype B1 Fusion (F) Glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus subtype A2 Fusion (F) Glycoprotein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 59 L1 protein with Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] ab322271 at 1/2000 (0.49ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 59 (HPV59) L1 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling mNeonGreen with Anti-mNeonGreen antibody [EPR28835-76] ab321887 at 1/2000 (0.249 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 2 ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a mNeonGreen-GPGPG-SP1 expression vector (shown in green). Anti-mNeonGreen antibody [EPR28835-76] ab321887 is shown in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  was used to counterstain tubulin at 1/None dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker ab109028 staining HP1 alpha in HeLa (human cervical adenocarcinoma epithelial cell). The cells were fixed with 100% methanol, permeabilized with 0.1% TritonX-100. The cells were then incubated with Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker ab109028 at 1:100 dilution. Cells were then incubated with ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1:1000 dilution (shown in green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as counterstain at 1:200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  
  Confocal image showing nuclear staining in HeLa cell line.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) cells labelling SARS-CoV-2 orf7a with primary antibody anti-SARS-CoV-2 orf7a (Anti-SARS-CoV-2 orf7a antibody [EPR24850-55] ab283962) at 1/100 dilution, followed by AlexaFluor®594 Goat anti-Rabbit (green) (ab150088) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with a SARS-CoV-2 orf7a and a SARS-CoV-1 orf7a expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-CoV-2 orf8 expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  Secondary antibody only control : Secondary antibody is ab150088 AlexaFluor®594 Goat anti-Rabbit (green) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Noradrenaline with Anti-Noradrenaline antibody [EPR24435-114] ab315969 at 1/50 (10.06 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 (2ug/ml) dilution (Magenta).

  Confocal image showing positive staining in RAW 264.7 cells treated with 0.5 mg/mL Norepinephrine-crosslink-BSA-FITC for 4 hours and no staining when 0.5 mg/mL crosslink-BSA-FITC was incubated for 4 hours.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  
  
  The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2ug/ml) dilution.

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  Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at 1/50 (10.18 ug/ml) dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-GPCR GPR17 (Anti-GPCR GPR17 antibody [EPR26423-34] ab316105, green), anti-Olig2 (Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] ab225099, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on mouse cerebellum.
  
  Panel B: anti-GPCR GPR17 stained on mouse cerebellum.
  
  Panel C: anti-Olig2 stained in oligodendrocyte of mouse cerebellum.
  
  Panel D: anti-GFAP stained in astrocytes of mouse cerebellum.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)at 1/1000 (2 ug/mL) dilution.

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  Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling GPCR GPR17 with Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at 1/50 (10.18 ug/ml) dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-GPCR GPR17 (Anti-GPCR GPR17 antibody [EPR26423-34] ab316105, green), anti-Olig2 (Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] ab225099, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on rat cerebellum.
  
  Panel B: anti-GPCR GPR17 stained on rat cerebellum.
  
  Panel C: anti-Olig2 stained in oligodendrocyte of rat cerebellum.
  
  Panel D: anti-GFAP stained in astrocytes of rat cerebellum.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)at 1/1000 (2 ug/mL) dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling SARS-CoV2 orf8 with primary antibody anti-SARS-CoV2 orf8 (Anti-SARS-CoV2 orf8 antibody [EPR24851-65] ab283914) at 1/100 dilution, followed by AlexaFluor®594 Goat anti-Rabbit (green) (ab150088) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with a SARS-CoV2 orf8 expression vector containing a myc tag, no staining in HEK-293T cells transfected with a SARS-COV2 orf7a expression vector containing a myc tag. Myc-Tag Mouse mab (Alexa Fluor® 647) (red) was used to counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized transfected 293T (human embryonic kidney epithelial cell) cells labelling HIV Vif with Anti-HIV1 Vif antibody [319] ab66643 at 1/100 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluo^® 488) at 1/1000 dilution (green).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed at a 1/1000 dilution (Magenta).

  Confocal image showing positive staining in 293T cells transfected with human immunodeficiency virus type 1 group M subtype B (isolate YU-2) (HIV-1) Vif expression vector containing a myc-His-tag^® (shown in green) but no staining in 293T cells transfected with a human immunodeficiency virus type 1 group M subtype K (isolate 96CM-MP535) (HIV-1) Vif, human immunodeficiency virus type 1 group M subtype K (isolate 97ZR-EQTB11) (HIV-1) Vif, human immunodeficiency virus type 1 group M subtype D (isolate ELI) (HIV-1) Vif expression vector containing a myc-His-tag^®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  A: 293T cells transfected with an empty vector containing a myc-His-tag^®
  B: 293T cells transfected with a human immunodeficiency virus type 1 group M subtype K (isolate 96CM-MP535) (HIV-1) Vif expression vector containing a myc-His-tag^®
  C: 293T cells transfected with a human immunodeficiency virus type 1 group M subtype K (isolate 97ZR-EQTB11) (HIV-1) Vif expression vector containing a myc-His-tag^®
  D: 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate YU-2) (HIV-1) Vif expression vector containing a myc-His-tag^®
  E: 293T cells transfected with a human immunodeficiency virus type 1 group M subtype D (isolate ELI) (HIV-1) Vif expression vector containing a myc-His-tag^®

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling GFP with Anti-GFP antibody [EPR28018-38] ab314656 at 1/50 (10.18 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining in 293T cells transfected with a empty expression vector containing a GFP tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 (2 ug/mL) dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus M1 protein with Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] ab322959 at 1/2000 (0.50 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1000 2ug/ml dilution (Green).

  Confocal image showing nuclear staining in 293T cells transfected with a Influenza A virus M1 protein expression vector containing a myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] ab322959 at 1/2000 dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

  -ve control 2: Anti-Myc tag antibody ab9106 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] ab322925 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

  Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1, (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 11 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
  

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with Anti-Human papillomavirus 6 L1 protein antibody [LH6L1_D] ab322925 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

  Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1 expression vector containing a myc tag (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 18 L1 or human papillomavirus type 4 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HPV4 L2 with Anti-Human papillomavirus type 4 L2 antibody [HPV4L2(20-38)_4SA] ab322958 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

  Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 4 L2 and Human papillomavirus type 65 L2 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a human papillomavirus type 60 L2 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus phosphoprotein VP32 with Anti-Human respiratory syncytial virus phosphoprotein VP32 antibody [3.5-18] ab322893 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

  Confocal image showing positive staining both in 293T cells transfected with a human respiratory syncytial virus A (strain A2) Phosphoprotein VP32 and a human respiratory syncytial virus B (strain B1) Phosphoprotein VP32 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with Anti-Human respiratory syncytial virus Fusion (F) Glycoprotein antibody [11-3-A3] ab322869 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a Human respiratory syncytial virus (RSV) (strain B1) Fusion glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus (RSV) (strain A2) Fusion glycoprotein expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).