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Goat anti-rabbit IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 430 publications


Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (AB150079), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (AB150079), expandable thumbnail
  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (AB150079), expandable thumbnail
  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (AB150079), expandable thumbnail
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (AB150079), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal

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Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/2000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

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Alternative names

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Goat anti-rabbit IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 430 publications

Key facts

Description
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Immunogen
Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RFP with Anti-RFP antibody [EPR28246-45] ab314225 at 1/2000 (0.252 ug/ml) dilution, followed by ab150079 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing positive staining in 293T cells transfected with a RFP expression vector containing a Myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain at 1/100 (0.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is ab150079 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) at 1/500 (4 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

    ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 2μg/ml) overnight at +4°C. The secondary antibody ab150079 (shown in red) was used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), expandable thumbnail

    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

    Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left)/ LNCaP (Human prostate carcinoma epithelial cell, Right) cells labelling TMPRSS2 with Anti-TMPRSS2 antibody [EPR24407-87] ab280567 at 1/500 dilution (0.1μg)/ red compared with Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-239T (PMID: 20631123).

    Gated on viable cells.

  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), expandable thumbnail

    Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), expandable thumbnail

    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

    Cross-reactivity of the polyclonal secondary antibody Goat Anti-Rabbit IgG H&L ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.

    For the batch tested, Goat Anti-Rabbit IgG H&L ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

    This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), expandable thumbnail

    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

    Cross-reactivity of Goat anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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