Goat anti-rabbit IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 430 publications
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Publications
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- Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 30:e135622024Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang Aixue,Wei Feng,Zhang Huanhuan,M Xixing,L YanlingPubMed 38279604
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e234012024Ferrostatin-1 inhibits ferroptosis of vascular smooth muscle cells and alleviates abdominal aortic aneurysm formation through activating the SLC7A11/GPX4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin He,Yunchuan Xiong,Yu Liu,Yaozhen Li,Haiyang Zhou,Kemin WuPubMed 38236196
- Progress in neurobiology 233:1025682024Tdrd3-null mice show post-transcriptional and behavioral impairments associated with neurogenesis and synaptic plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingliang Zhu,Yuyoung Joo,Simone Bossi,Ross A McDevitt,Aoji Xie,Yue Wang,Yutong Xue,Shuaikun Su,Seung Kyu Lee,Nirnath Sah,Shiliang Zhang,Rong Ye,Alejandro Pinto,Yongqing Zhang,Kimi Araki,Masatake Araki,Marisela Morales,Mark P Mattson,Henriette van Praag,Weidong WangPubMed 38216113
- iScience 27:1086702023Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Hennegan,Aled H Bryant,Lauren Griffiths,Matthieu Trigano,Oliver J M Bartley,Joanna J Bartlett,Carys Minahan,Willy Antoni Abreu de Oliveira,Eylan Yutuc,Sotirios Ntikas,Christos S Bartsocas,Margarita Markouri,Eleni Antoniadou,Ioanna Laina,Owain W Howell,Meng Li,Yuqin Wang,William J Griffiths,Emma L Lane,Mariah J Lelos,Spyridon TheofilopoulosPubMed 38155767
- Mobile DNA 14:202023An intronic LINE-1 regulates IFNAR1 expression in human immune cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen A Buttler,Daniel Ramirez,Robin D Dowell,Edward B ChuongPubMed 38037122
- Science advances 9:eadk10502023Biochemically functionalized probes for cell-type-specific targeting and recording in the brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnqi Zhang,Theodore J Zwang,Charles M LieberPubMed 38019917
- Cancer cell international 23:2862023CDK5 promotes apoptosis and attenuates chemoresistance in gastric cancer via E2F1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Long Cao,Yu-Kai Wu,Tong-Xin Lin,Mi Lin,Yu-Jing Chen,Ling-Qian Wang,Jia-Bin Wang,Jian-Xian Lin,Jun Lu,Qi-Yue Chen,Ru-Hong Tu,Ze-Ning Huang,Ju-Li Lin,Hua-Long Zheng,Jian-Wei Xie,Ping Li,Chang-Ming Huang,Chao-Hui ZhengPubMed 37990321
- Frontiers in immunology 14:12716032023Hypoxia mediates immune escape of pancreatic cancer cells by affecting miR-1275/AXIN2 in natural killer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenglin Ou,Yebin Lu,Dayong Xu,Zhen LuoPubMed 38035113
- Heliyon 9:e223062023Nanobead-based single-molecule pulldown for single cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQirui Zhao,Yusheng Shen,Xiaofen Li,Yulin Li,Fang Tian,Xiaojie Yu,Zhengzhao Liu,Rongbiao Tong,Hyokeun Park,Levent Yobas,Pingbo HuangPubMed 38027957
- Cardiovascular diabetology 22:2932023Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Jiang,Chun Gan,Xindi Zhou,Qing Yang,Dan Chen,Han Xiao,Lujun Dai,Yaxi Chen,Mo Wang,Haiping Yang,Qiu LiPubMed 37891556
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Goat anti-rabbit IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 430 publications
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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6 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RFP with Anti-RFP antibody [EPR28246-45] ab314225 at 1/2000 (0.252 ug/ml) dilution, followed by ab150079 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing positive staining in 293T cells transfected with a RFP expression vector containing a Myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain at 1/100 (0.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150079 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) at 1/500 (4 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 2μg/ml) overnight at +4°C. The secondary antibody ab150079 (shown in red) was used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left)/ LNCaP (Human prostate carcinoma epithelial cell, Right) cells labelling TMPRSS2 with Anti-TMPRSS2 antibody [EPR24407-87] ab280567 at 1/500 dilution (0.1μg)/ red compared with Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK-239T (PMID: 20631123).
Gated on viable cells.
Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
Cross-reactivity of the polyclonal secondary antibody Goat Anti-Rabbit IgG H&L ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.
For the batch tested, Goat Anti-Rabbit IgG H&L ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.
This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).
ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
Cross-reactivity of Goat anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).
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