AB150087

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 647
Brand: Abcam Limited
SKU: AB150087
Price: 255 USD
Availability: InStock

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(11 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150087 Alexa Fluor® 647 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ELISA, IHC-P, Flow Cyt, IHC-Fr, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody." }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6569 PubMed37848444

2023

Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Wei Chou,Chia-Nung Hung,Cheryl Hsiang-Ling Chiu,Xi Tan,Meizhen Chen,Chien-Chin Chen,Moawiz Saeed,Che-Wei Hsu,Michael A Liss,Chiou-Miin Wang,Zhao Lai,Nathaniel Alvarez,Pawel A Osmulski,Maria E Gaczynska,Li-Ling Lin,Veronica Ortega,Nameer B Kirma,Kexin Xu,Zhijie Liu,Addanki P Kumar,Josephine A Taverna,Gopalrao V N Velagaleti,Chun-Liang Chen,Zhao Zhang,Tim Hui-Ming Huang

Nature communications 13:3997 PubMed35810181

2022

Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Xin Wen,Zhengmao Wang,Zaisheng Lin,Chunhong Li,Huilin Zhou,Huimin Yu,Yuhan Li,Yifei Cheng,Yuling Chen,Geer Lou,Junmin Pan,Muqing Cao

Viruses 14: PubMed35336863

2022

Characterization of Hantavirus N Protein Intracellular Dynamics and Localization.

Applications

Unspecified application

Species

Unspecified reactive species

Robert-William Welke,Hannah Sabeth Sperber,Ronny Bergmann,Amit Koikkarah,Laura Menke,Christian Sieben,Detlev H Krüger,Salvatore Chiantia,Andreas Herrmann,Roland Schwarzer

Nature immunology 22:880-892 PubMed34099917

2021

Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hiltensperger,Eduardo Beltrán,Ravi Kant,Sofia Tyystjärvi,Gildas Lepennetier,Helena Domínguez Moreno,Isabel J Bauer,Simon Grassmann,Sebastian Jarosch,Kilian Schober,Veit R Buchholz,Selin Kenet,Christiane Gasperi,Rupert Öllinger,Roland Rad,Andreas Muschaweckh,Christopher Sie,Lilian Aly,Benjamin Knier,Garima Garg,Ali M Afzali,Lisa Ann Gerdes,Tania Kümpfel,Sören Franzenburg,Naoto Kawakami,Bernhard Hemmer,Dirk H Busch,Thomas Misgeld,Klaus Dornmair,Thomas Korn

International journal of molecular sciences 22: PubMed34065402

2021

KRAS and EGFR Mutations Differentially Alter ABC Drug Transporter Expression in Cisplatin-Resistant Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Jaromi,Veronika Csongei,Monika Vesel,ElHusseiny Mohamed Mahmud Abdelwahab,Amina Soltani,Zsofia Torok,Gabor Smuk,Veronika Sarosi,Judit Erzsebet Pongracz

The EMBO journal 40:e106336 PubMed33595114

2021

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Raghunandan,Dan Geelen,Eva Majerova,Anabelle Decottignies

Brain structure & function 226:365-379 PubMed33398432

2021

Loss of α7 nicotinic acetylcholine receptors in GABAergic neurons causes sex-dependent decreases in radial glia-like cell quantity and impairments in cognitive and social behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Samir A Nacer,Ayland C Letsinger,Simone Otto,Jemma Strauss DeFilipp,Viktoriya D Nikolova,Natallia V Riddick,Korey D Stevanovic,Jesse D Cushman,Jerrel L Yakel

Lipids in health and disease 19:54 PubMed32213192

2020

Bioinformatics analysis of vascular RNA-seq data revealed hub genes and pathways in a novel Tibetan minipig atherosclerosis model induced by a high fat/cholesterol diet.

Applications

Unspecified application

Species

Unspecified reactive species

Yongming Pan,Chen Yu,Junjie Huang,Yili Rong,Jiaojiao Chen,Minli Chen

Cell metabolism 29:1243-1257.e10 PubMed30827861

2019

GAPDH Expression Predicts the Response to R-CHOP, the Tumor Metabolic Status, and the Response of DLBCL Patients to Metabolic Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Chiche,Julie Reverso-Meinietti,Annabelle Mouchotte,Camila Rubio-Patiño,Rana Mhaidly,Elodie Villa,Jozef P Bossowski,Emma Proics,Manuel Grima-Reyes,Agnès Paquet,Konstantina Fragaki,Sandrine Marchetti,Josette Briere,Damien Ambrosetti,Jean-François Michiels,Thierry Jo Molina,Christiane Copie-Bergman,Jacqueline Lehmann-Che,Isabelle Peyrottes,Frederic Peyrade,Eric de Kerviler,Bruno Taillan,Georges Garnier,Els Verhoeyen,Véronique Paquis-Flucklinger,Laetitia Shintu,Vincent Delwail,Celine Delpech-Debiais,Richard Delarue,André Bosly,Tony Petrella,Gabriel Brisou,Bertrand Nadel,Pascal Barbry,Nicolas Mounier,Catherine Thieblemont,Jean-Ehrland Ricci

Developmental cell 46:344-359.e4 PubMed30032992

2018

Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey P Rasmussen,Nhat-Thi Vo,Alvaro Sagasti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

Product promise