AB150087

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 647
Brand: Abcam Limited
SKU: AB150087
Price: 255 USD
Availability: InStock

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(17 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 17 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150087 Alexa Fluor® 647 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ICC/IF, Flow Cyt, ELISA, IHC-Fr, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody." }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 10:53 PubMed38763950

2024

DEPDC5 protects CD8 T cells from ferroptosis by limiting mTORC1-mediated purine catabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Song Li,Xinxing Ouyang,Hongxiang Sun,Jingsi Jin,Yao Chen,Liang Li,Qijun Wang,Yingzhong He,Jiwen Wang,Tongxin Chen,Qing Zhong,Yinming Liang,Philippe Pierre,Qiang Zou,Youqiong Ye,Bing Su

Nature communications 15:2382 PubMed38493217

2024

Maternal dietary fat during lactation shapes single nucleus transcriptomic profile of postnatal offspring hypothalamus in a sexually dimorphic manner in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Huang,Anyongqi Wang,Wenjiang Zhou,Baoguo Li,Linshan Zhang,Agata M Rudolf,Zengguang Jin,Catherine Hambly,Guanlin Wang,John R Speakman

Nature 627:407-415 PubMed38383779

2024

B cells orchestrate tolerance to the neuromyelitis optica autoantigen AQP4.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Maisam Afzali,Lucy Nirschl,Christopher Sie,Monika Pfaller,Oleksii Ulianov,Tobias Hassler,Christine Federle,Elisabetta Petrozziello,Sudhakar Reddy Kalluri,Hsin Hsiang Chen,Sofia Tyystjärvi,Andreas Muschaweckh,Katja Lammens,Claire Delbridge,Andreas Büttner,Katja Steiger,Gönül Seyhan,Ole Petter Ottersen,Rupert Öllinger,Roland Rad,Sebastian Jarosch,Adrian Straub,Anton Mühlbauer,Simon Grassmann,Bernhard Hemmer,Jan P Böttcher,Ingrid Wagner,Mario Kreutzfeldt,Doron Merkler,Irene Bonafonte Pardàs,Marc Schmidt Supprian,Veit R Buchholz,Sylvia Heink,Dirk H Busch,Ludger Klein,Thomas Korn

Nature communications 14:6569 PubMed37848444

2023

Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Wei Chou,Chia-Nung Hung,Cheryl Hsiang-Ling Chiu,Xi Tan,Meizhen Chen,Chien-Chin Chen,Moawiz Saeed,Che-Wei Hsu,Michael A Liss,Chiou-Miin Wang,Zhao Lai,Nathaniel Alvarez,Pawel A Osmulski,Maria E Gaczynska,Li-Ling Lin,Veronica Ortega,Nameer B Kirma,Kexin Xu,Zhijie Liu,Addanki P Kumar,Josephine A Taverna,Gopalrao V N Velagaleti,Chun-Liang Chen,Zhao Zhang,Tim Hui-Ming Huang

Science advances 9:eadi1870 PubMed37556541

2023

Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Anqi Zhang,Kang Yong Loh,Chandan S Kadur,Lukas Michalek,Jiayi Dou,Charu Ramakrishnan,Zhenan Bao,Karl Deisseroth

Tissue barriers 12:2184157 PubMed36852963

2023

Yogurt starter strains ameliorate intestinal barrier dysfunction via activating AMPK in Caco-2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyosuke Kobayashi,Junko Mochizuki,Fuka Yamazaki,Toshihiro Sashihara

Nature communications 13:3997 PubMed35810181

2022

Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Xin Wen,Zhengmao Wang,Zaisheng Lin,Chunhong Li,Huilin Zhou,Huimin Yu,Yuhan Li,Yifei Cheng,Yuling Chen,Geer Lou,Junmin Pan,Muqing Cao

Viruses 14: PubMed35336863

2022

Characterization of Hantavirus N Protein Intracellular Dynamics and Localization.

Applications

Unspecified application

Species

Unspecified reactive species

Robert-William Welke,Hannah Sabeth Sperber,Ronny Bergmann,Amit Koikkarah,Laura Menke,Christian Sieben,Detlev H Krüger,Salvatore Chiantia,Andreas Herrmann,Roland Schwarzer

Nature immunology 22:880-892 PubMed34099917

2021

Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hiltensperger,Eduardo Beltrán,Ravi Kant,Sofia Tyystjärvi,Gildas Lepennetier,Helena Domínguez Moreno,Isabel J Bauer,Simon Grassmann,Sebastian Jarosch,Kilian Schober,Veit R Buchholz,Selin Kenet,Christiane Gasperi,Rupert Öllinger,Roland Rad,Andreas Muschaweckh,Christopher Sie,Lilian Aly,Benjamin Knier,Garima Garg,Ali M Afzali,Lisa Ann Gerdes,Tania Kümpfel,Sören Franzenburg,Naoto Kawakami,Bernhard Hemmer,Dirk H Busch,Thomas Misgeld,Klaus Dornmair,Thomas Korn

International journal of molecular sciences 22: PubMed34065402

2021

KRAS and EGFR Mutations Differentially Alter ABC Drug Transporter Expression in Cisplatin-Resistant Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Jaromi,Veronika Csongei,Monika Vesel,ElHusseiny Mohamed Mahmud Abdelwahab,Amina Soltani,Zsofia Torok,Gabor Smuk,Veronika Sarosi,Judit Erzsebet Pongracz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150087)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (17)

Product promise