Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.
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- Nature communications 14:65692023Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Wei Chou,Chia-Nung Hung,Cheryl Hsiang-Ling Chiu,Xi Tan,Meizhen Chen,Chien-Chin Chen,Moawiz Saeed,Che-Wei Hsu,Michael A Liss,Chiou-Miin Wang,Zhao Lai,Nathaniel Alvarez,Pawel A Osmulski,Maria E Gaczynska,Li-Ling Lin,Veronica Ortega,Nameer B Kirma,Kexin Xu,Zhijie Liu,Addanki P Kumar,Josephine A Taverna,Gopalrao V N Velagaleti,Chun-Liang Chen,Zhao Zhang,Tim Hui-Ming HuangPubMed 37848444
- Nature communications 13:39972022Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Wang,Xin Wen,Zhengmao Wang,Zaisheng Lin,Chunhong Li,Huilin Zhou,Huimin Yu,Yuhan Li,Yifei Cheng,Yuling Chen,Geer Lou,Junmin Pan,Muqing CaoPubMed 35810181
- Viruses 14:2022Characterization of Hantavirus N Protein Intracellular Dynamics and Localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert-William Welke,Hannah Sabeth Sperber,Ronny Bergmann,Amit Koikkarah,Laura Menke,Christian Sieben,Detlev H Krüger,Salvatore Chiantia,Andreas Herrmann,Roland SchwarzerPubMed 35336863
- Nature immunology 22:880-8922021Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Hiltensperger,Eduardo Beltrán,Ravi Kant,Sofia Tyystjärvi,Gildas Lepennetier,Helena Domínguez Moreno,Isabel J Bauer,Simon Grassmann,Sebastian Jarosch,Kilian Schober,Veit R Buchholz,Selin Kenet,Christiane Gasperi,Rupert Öllinger,Roland Rad,Andreas Muschaweckh,Christopher Sie,Lilian Aly,Benjamin Knier,Garima Garg,Ali M Afzali,Lisa Ann Gerdes,Tania Kümpfel,Sören Franzenburg,Naoto Kawakami,Bernhard Hemmer,Dirk H Busch,Thomas Misgeld,Klaus Dornmair,Thomas KornPubMed 34099917
- International journal of molecular sciences 22:2021KRAS and EGFR Mutations Differentially Alter ABC Drug Transporter Expression in Cisplatin-Resistant Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Jaromi,Veronika Csongei,Monika Vesel,ElHusseiny Mohamed Mahmud Abdelwahab,Amina Soltani,Zsofia Torok,Gabor Smuk,Veronika Sarosi,Judit Erzsebet PongraczPubMed 34065402
- The EMBO journal 40:e1063362021NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaya Raghunandan,Dan Geelen,Eva Majerova,Anabelle DecottigniesPubMed 33595114
- Brain structure & function 226:365-3792021Loss of α7 nicotinic acetylcholine receptors in GABAergic neurons causes sex-dependent decreases in radial glia-like cell quantity and impairments in cognitive and social behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamir A Nacer,Ayland C Letsinger,Simone Otto,Jemma Strauss DeFilipp,Viktoriya D Nikolova,Natallia V Riddick,Korey D Stevanovic,Jesse D Cushman,Jerrel L YakelPubMed 33398432
- Lipids in health and disease 19:542020Bioinformatics analysis of vascular RNA-seq data revealed hub genes and pathways in a novel Tibetan minipig atherosclerosis model induced by a high fat/cholesterol diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongming Pan et. al.PubMed 32213192
- Cell metabolism 29:1243-1257.e102019GAPDH Expression Predicts the Response to R-CHOP, the Tumor Metabolic Status, and the Response of DLBCL Patients to Metabolic Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna Chiche,Julie Reverso-Meinietti,Annabelle Mouchotte,Camila Rubio-Patiño,Rana Mhaidly,Elodie Villa,Jozef P Bossowski,Emma Proics,Manuel Grima-Reyes,Agnès Paquet,Konstantina Fragaki,Sandrine Marchetti,Josette Briere,Damien Ambrosetti,Jean-François Michiels,Thierry Jo Molina,Christiane Copie-Bergman,Jacqueline Lehmann-Che,Isabelle Peyrottes,Frederic Peyrade,Eric de Kerviler,Bruno Taillan,Georges Garnier,Els Verhoeyen,Véronique Paquis-Flucklinger,Laetitia Shintu,Vincent Delwail,Celine Delpech-Debiais,Richard Delarue,André Bosly,Tony Petrella,Gabriel Brisou,Bertrand Nadel,Pascal Barbry,Nicolas Mounier,Catherine Thieblemont,Jean-Ehrland RicciPubMed 30827861
- Developmental cell 46:344-359.e42018Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey P Rasmussen et. al.PubMed 30032992
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Horse, Chicken, Cow, Human, Pig
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody. |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Horse, Chicken, Cow, Human, Pig
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150087)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150087 Alexa Fluor® 647 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150087)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150087)
Anti-160 kD Neurofilament Medium antibody [EP2460] - Neuronal Marker ab92539 stained in SHSY5Y cells. Cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with the antibody Anti-160 kD Neurofilament Medium antibody [EP2460] - Neuronal Marker ab92539 at 0.5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150087 (colored red) and Donkey Anti-Sheep IgG H&L (Alexa Fluor® 488) ab150177 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
This product gave a positive signal in 4% formaldehyde (10 min) fixed SHSY5Y cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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