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AB175773

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

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(19 Publications)

Suitable for WB. Cited in 19 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (AB175773)
  • ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (AB175773)

Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

This data was developed using the unconjugated antibody (ab182016).

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (AB175773)
  • ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (AB175773)

Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

This data was developed using the unconjugated antibody (ab182016).

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (AB175773)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (AB175773)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using ab175773 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-loading-control-ab18251'>ab18251</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Applications

WB

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680), ab175773 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680), ab175773 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed38139101

2023

A Comparative Study of Different Protocols for Isolation of Murine Neutrophils from Bone Marrow and Spleen.

Applications

Unspecified application

Species

Unspecified reactive species

Khetam Sounbuli,Ludmila A Alekseeva,Oleg V Markov,Nadezhda L Mironova

Molecular cancer therapeutics 22:306-316 PubMed36622760

2023

Nifuroxazide Activates the Parthanatos to Overcome TMPRSS2:ERG Fusion-Positive Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengxun Li,Jiale Zhang,Qiming Wu,Anuj Kumar,Guihong Pan,David J Kelvin

Scientific reports 12:17280 PubMed36241900

2022

Colocalization of senescent biomarkers in deep, superficial, and ovarian endometriotic lesions: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Palmieri,Helena Malvezzi,Bruna Cestari,Sergio Podgaec

Frontiers in oncology 12:976329 PubMed36119544

2022

Urinary exosomal long non-coding RNAs as noninvasive biomarkers for diagnosis of bladder cancer by RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Bingxian Bian,Li Li,Xing Ke,Hui Chen,Yi Liu,Naisheng Zheng,Yingxia Zheng,Yanhui Ma,Yunlan Zhou,Junyao Yang,Lanshu Xiao,Lisong Shen

Cell host & microbe 30:809-823.e6 PubMed35439436

2022

High-fat diet disrupts REG3γ and gut microbial rhythms promoting metabolic dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Katya Frazier,Amal Kambal,Elizabeth A Zale,Joseph F Pierre,Nathaniel Hubert,Sawako Miyoshi,Jun Miyoshi,Daina L Ringus,Dylan Harris,Karen Yang,Katherine Carroll,Jake B Hermanson,John S Chlystek,Katherine A Overmyer,Candace M Cham,Mark W Musch,Joshua J Coon,Eugene B Chang,Vanessa A Leone

Journal of molecular biology 434:167478 PubMed35123996

2022

A Similarity-Based Method for Predicting Enzymatic Functions in Yeast Uncovers a New AMP Hydrolase.

Applications

Unspecified application

Species

Unspecified reactive species

Nir Cohen,Amit Kahana,Maya Schuldiner

PloS one 16:e0262180 PubMed34972198

2021

The TOR kinase pathway is relevant for nitrogen signaling and antagonism of the mycoparasite Trichoderma atroviride.

Applications

Unspecified application

Species

Unspecified reactive species

Rossana Segreto,Hoda Bazafkan,Julia Millinger,Martina Schenk,Lea Atanasova,Maria Doppler,Christoph Büschl,Mélanie Boeckstaens,Silvia Soto Diaz,Ulrike Schreiner,Fabiano Sillo,Raffaella Balestrini,Rainer Schuhmacher,Susanne Zeilinger

Cell host & microbe 29:1316-1332.e12 PubMed34237247

2021

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Walch,Joel Selkrig,Leigh A Knodler,Mandy Rettel,Frank Stein,Keith Fernandez,Cristina Viéitez,Clément M Potel,Karoline Scholzen,Matthias Geyer,Klemens Rottner,Olivia Steele-Mortimer,Mikhail M Savitski,David W Holden,Athanasios Typas

Oncogenesis 9:92 PubMed33070167

2020

Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Te Zhang,Xuming Song,Zeyu Zhang,Qixing Mao,Wenjie Xia,Lin Xu,Feng Jiang,Gaochao Dong

mBio 11: PubMed32788377

2020

Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Zouhir,Wiem Abidi,Meryem Caleechurn,Petya Violinova Krasteva
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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