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AB186696

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

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(5 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

1 Images
Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (AB186696)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (AB186696)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab186696 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186696) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Applications

WB

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed, ab186696 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed, ab186696 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:5266-5278 PubMed37354210

2023

Hepatic hydrogen sulfide levels are reduced in mouse model of Hutchinson-Gilford progeria syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen E Wilkie,Diana E Marcu,Roderick N Carter,Nicholas M Morton,Susana Gonzalo,Colin Selman

Cell reports 39:110698 PubMed35443182

2022

Bladder epithelial cell phosphate transporter inhibition protects mice against uropathogenic Escherichia coli infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Pang,Zhihui Cheng,Si Zhang,Shujie Li,Xueping Li,Xiaodan Li,Xiao Zhang,Xiaoxiao Li,Yingxing Feng,Heting Cui,Zhen Chen,Le Liu,Qing Li,Jianxiao Huang,Mingqing Zhang,Siwei Zhu,Lei Wang,Lu Feng

Frontiers in immunology 13:827709 PubMed35401540

2022

Swine Interferon-Inducible Transmembrane Proteins Potently Inhibit African Swine Fever Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Cai,Zezhong Zheng,JiaoJiao Cheng,Lintao Zhong,Ran Shao,Feiyan Zheng,Zhiying Lai,Jiajun Ou,Liang Xu,Pei Zhou,Gang Lu,Guihong Zhang

Scientific reports 8:11528 PubMed30068998

2018

The Metabolomic Bioenergetic Signature of Opa1-Disrupted Mouse Embryonic Fibroblasts Highlights Aspartate Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Cinzia Bocca,Mariame Selma Kane,Charlotte Veyrat-Durebex,Stéphanie Chupin,Jennifer Alban,Judith Kouassi Nzoughet,Morgane Le Mao,Juan Manuel Chao de la Barca,Patrizia Amati-Bonneau,Dominique Bonneau,Vincent Procaccio,Guy Lenaers,Gilles Simard,Arnaud Chevrollier,Pascal Reynier

Oncotarget 8:105155-105169 PubMed29285241

2017

GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shicong Tang,Hong Pan,Wei Wei,Huawei Yang,Jianlun Liu,Rirong Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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