AB175733

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 750
Brand: Abcam Limited
SKU: AB175733
Price: 280 USD
Availability: InStock

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(10 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.

2 Images

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175733)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175733 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175733) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175733)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175733)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26814 PubMed40702232

2025

Cell-specific α and β subunit expression patterns of Na/K-ATPases in the common marmoset cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masafumi Ueno,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hiroyuki Ozawa

Scientific reports 14:25166 PubMed39448766

2024

A primate model animal revealed the inter-species differences and similarities in the subtype specifications of the spiral ganglion neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masafumi Ueno,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hiroyuki Ozawa

Molecular and cellular biology 44:411-428 PubMed39169784

2024

Unveiling the Role of Sik1 in Osteoblast Differentiation: Implications for Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kuanmin Tian,Xiaoxin He,Xue Lin,Xiaolei Chen,Yajing Su,Zhidong Lu,Zhirong Chen,Liang Zhang,Peng Li,Long Ma,Zhibin Lan,Xin Zhao,Gangning Fen,Qinqin Hai,Di Xue,Qunhua Jin

Pediatric transplantation 27:e14475 PubMed36691289

2023

T-cell infiltrate intensity is associated with delayed response to treatment in late acute cellular rejection in pediatric liver transplant recipients.

Applications

Unspecified application

Species

Unspecified reactive species

Anna L Peters,Michael Rogers,Gousia Begum,Qin Sun,Lin Fei,Daniel Leino,David Hildeman,Ervin Steve Woodle

Scientific reports 11:16512 PubMed34389795

2021

Mitochondrial dysfunction associated with autophagy and mitophagy in cerebrospinal fluid cells of patients with delayed cerebral ischemia following subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Hyuk Youn,Youngmi Kim,Bong Jun Kim,Myeong Seon Jeong,Jooeun Lee,Jong Kook Rhim,Heung Cheol Kim,Jin Pyeong Jeon

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12577-12598 PubMed32677089

2020

Endoplasmic reticulum stress in the dorsal root ganglia regulates large-conductance potassium channels and contributes to pain in a model of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Saad Yousuf,Samira Samtleben,Shawn M Lamothe,Timothy N Friedman,Ana Catuneanu,Kevin Thorburn,Mansi Desai,Gustavo Tenorio,Geert J Schenk,Klaus Ballanyi,Harley T Kurata,Thomas Simmen,Bradley J Kerr

Cell death & disease 11:372 PubMed32415085

2020

A circular RNA derived from DAB1 promotes cell proliferation and osteogenic differentiation of BMSCs via RBPJ/DAB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weekai Chia,Jiali Liu,Yi-Gang Huang,Changqing Zhang

Cell and tissue research 381:55-69 PubMed32036485

2020

Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Timo Schomann,Juvita D Iljas,Ivo Que,Yuedan Li,Ernst Suidgeest,Luis J Cruz,Johan H M Frijns,Alan Chan,Clemens M W G Löwik,Margriet A Huisman,Laura Mezzanotte

Cancer research 78:6399-6412 PubMed30254148

2018

An ATM/TRIM37/NEMO Axis Counteracts Genotoxicity by Activating Nuclear-to-Cytoplasmic NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Geyan Wu,Libing Song,Jinrong Zhu,Yameng Hu,Lixue Cao,Zhanyao Tan,Shuxia Zhang,Ziwen Li,Jun Li

eLife 6: PubMed28656888

2017

The complex of TRIP-Br1 and XIAP ubiquitinates and degrades multiple adenylyl cyclase isoforms.

Applications

ICC/IF

Species

Unspecified reactive species

Wenbao Hu,Xiaojie Yu,Zhengzhao Liu,Ying Sun,Xibing Chen,Xin Yang,Xiaofen Li,Wai Kwan Lam,Yuanyuan Duan,Xu Cao,Hermann Steller,Kai Liu,Pingbo Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175733)

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175733)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

Product promise