Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

17 product images

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-alpha Tubulin antibody - Loading Control ab18251 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody - Loading Control ab18251) at 1 µg/mL

  All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 36 kDa

  Observed band size: 50 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Histone H2B (formyl K5) antibody ab177138 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-Histone H2B (formyl K5) antibody (Anti-Histone H2B (formyl K5) antibody ab177138) at 1 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

  Lane 3: NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg

  Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 14 kDa

  Observed band size: 18 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-beta Tubulin antibody [EP1331Y] - Loading Control (Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901) at 1/20000 dilution

  Lane 1: Brain (Mouse) Tissue Lysate at 20 µg

  Lane 2: Brain (Rat) Tissue Lysate at 20 µg

  Lane 3: Spinal Cord (Mouse) Tissue Lysate at 20 µg

  Lane 4: Spinal Cord (Rat) Tissue Lysate at 20 µg

  Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 49 kDa

  Observed band size: 52 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-beta Actin antibody - Loading Control ab8227 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-beta Actin antibody - Loading Control (Anti-beta Actin antibody - Loading Control ab8227) at 1/1000 dilution

  Lane 1: A431 (human epidermoid carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) Whole Cell Lysate at 20 µg

  Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg

  Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 41 kDa

  Observed band size: 42 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade ab175007 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade (Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade ab175007) at 1 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

  Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 14 kDa, 15 kDa, 36 kDa, 41 kDa, 49 kDa

  Observed band size: 17 kDa, 18 kDa, 37 kDa, 42 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Integrin beta 1 antibody [EP1041Y] ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  Secondary antibody - anti-rabbit Alexa Fluor 790, ab175781.

  All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (Anti-Integrin beta 1 antibody [EP1041Y] ab52971) at 20 µg

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

  Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 88 kDa

  Observed band size: 120 kDa, 140 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-alpha Tubulin antibody - Loading Control ab18251 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor^® 790 (ab175781) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody - Loading Control ab18251) at 0.5 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg

  Lane 2: HEK-293 (Human embryonic kidney cell line) whole cell lysate at 20 µg

  Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate at 20 µg

  Lane 4: Caco-2 (Human colonic carcinoma cell line) whole cell lysate at 20 µg

  Lane 5: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

  Lane 6: PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 27 kDa, 50 kDa

  Observed band size: 44 kDa, 52 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin beta 1 antibody [EP1041Y] ab52971).
  

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Integrin beta 1 antibody [EP1041Y] ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  Secondary antibody -anti-rabbit Alexa Fluor 790, ab175781

  All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free (Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free ab192456) at 20 µg

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

  Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 88 kDa

  Observed band size: 120 kDa, 140 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-muscle Actin antibody [EPR8484] - Loading Control ab156302 overnight at 4°C. Antibody binding was detected using ab175781 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  Secondary antibody - goat anti-rabbit Alexa Fluor^® 790 (ab175781).

  All lanes: Western blot - Anti-muscle Actin antibody [EPR8484] - Loading Control (Anti-muscle Actin antibody [EPR8484] - Loading Control ab156302) at 1/1000 dilution

  Lane 1: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

  Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 5: Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg

  Lane 6: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 42 kDa

  Observed band size: 42 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-ERK1 antibody [Y72] ab32537 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit Alexa Fluor^® 790) ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-ERK1 antibody [Y72] (Anti-ERK1 antibody [Y72] ab32537) at 1/1000 dilution

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

  Lane 3: Recombinant Human ERK1 protein (ab43623) (ab43623) at 20 µg

  Lane 4: Western blot - Recombinant Human ERK2 protein (Recombinant Human ERK2 protein ab43625) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 43 kDa

  Observed band size: 44 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-ERK2 antibody [E460] ab32081 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-ERK2 antibody [E460] (Anti-ERK2 antibody [E460] ab32081) at 1/1000 dilution

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

  Lane 3: 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 20 µg

  Lane 4: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

  Lane 5: Recombinant Human ERK1 protein (ab43623) (ab43623) at 20 µg

  Lane 6: Western blot - Recombinant Human ERK2 protein (Recombinant Human ERK2 protein ab43625) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 41 kDa

  Observed band size: 41 kDa

• 

  ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  Cross-reactivity of the polyclonal secondary antibody Goat Anti-Rabbit IgG H&L ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.
  

  For the batch tested, Goat Anti-Rabbit IgG H&L ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

  This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).

• 

  ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  Cross-reactivity of Goat anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
  

  This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 overnight at 4°C.
  

  Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623) at 1/1000 dilution

  Lane 1: Brain (Mouse) Tissue Lysate at 20 µg

  Lane 2: Brain (Rat) Tissue Lysate at 20 µg

  Lane 3: Spinal Cord (Mouse) Tissue Lysate at 20 µg

  Lane 4: Spinal Cord (Rat) Tissue Lysate at 20 µg

  Lane 5: PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 50 kDa

  Observed band size: 52 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor® 790 (ab175781) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) at 1/1000 dilution

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

  Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 4: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 5: NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

  Lane 6: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 50 kDa

  Observed band size: 52 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Histone H2B (formyl K108) antibody ab177168 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-Histone H2B (formyl K108) antibody (Anti-Histone H2B (formyl K108) antibody ab177168) at 1 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

  Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Predicted band size: 14 kDa

  Observed band size: 18 kDa, 37 kDa

• 

  Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Caspase-3 antibody [31A1067] ab13585 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Anti-MDC1 antibody (ab13858) at 1 µg/mL

  Lane 1: Hela whole cell lysate (Staurosporine treated, 2uM/4hr) at 20 µg

  Lane 2: Hela whole cell lysate (untreated control) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 22 kDa, 28 kDa, 29 kDa, 31 kDa, 50 kDa

  Observed band size: 17 kDa, 19 kDa, 32 kDa, 36 kDa, 43 kDa