Suitable for WB. Cited in 24 publications.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
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Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Suitable for WB. Cited in 24 publications.
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790), ab175781 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790), ab175781 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-alpha Tubulin antibody - Loading Control ab18251 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody - Loading Control ab18251) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Histone H2B (formyl K5) antibody ab177138 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Histone H2B (formyl K5) antibody (Anti-Histone H2B (formyl K5) antibody ab177138) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg
Lane 3: NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg
Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 18 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-beta Tubulin antibody [EP1331Y] - Loading Control (Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901) at 1/20000 dilution
Lane 1: Brain (Mouse) Tissue Lysate at 20 µg
Lane 2: Brain (Rat) Tissue Lysate at 20 µg
Lane 3: Spinal Cord (Mouse) Tissue Lysate at 20 µg
Lane 4: Spinal Cord (Rat) Tissue Lysate at 20 µg
Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 52 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-beta Actin antibody - Loading Control ab8227 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (Anti-beta Actin antibody - Loading Control ab8227) at 1/1000 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) Whole Cell Lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade ab175007 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade (Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade ab175007) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 14 kDa, 15 kDa, 36 kDa, 41 kDa, 49 kDa
Observed band size: 17 kDa, 18 kDa, 37 kDa, 42 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Integrin beta 1 antibody [EP1041Y] ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Secondary antibody - anti-rabbit Alexa Fluor 790, ab175781.
All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (Anti-Integrin beta 1 antibody [EP1041Y] ab52971) at 20 µg
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg
Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 88 kDa
Observed band size: 120 kDa, 140 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-alpha Tubulin antibody - Loading Control ab18251 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor® 790 (ab175781) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody - Loading Control ab18251) at 0.5 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human embryonic kidney cell line) whole cell lysate at 20 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate at 20 µg
Lane 4: Caco-2 (Human colonic carcinoma cell line) whole cell lysate at 20 µg
Lane 5: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg
Lane 6: PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 27 kDa, 50 kDa
Observed band size: 44 kDa, 52 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin beta 1 antibody [EP1041Y] ab52971).
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Integrin beta 1 antibody [EP1041Y] ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Secondary antibody -anti-rabbit Alexa Fluor 790, ab175781
All lanes: Western blot - Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free (Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free ab192456) at 20 µg
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg
Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 88 kDa
Observed band size: 120 kDa, 140 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-muscle Actin antibody [EPR8484] - Loading Control ab156302 overnight at 4°C. Antibody binding was detected using ab175781 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Secondary antibody - goat anti-rabbit Alexa Fluor® 790 (ab175781).
All lanes: Western blot - Anti-muscle Actin antibody [EPR8484] - Loading Control (Anti-muscle Actin antibody [EPR8484] - Loading Control ab156302) at 1/1000 dilution
Lane 1: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 5: Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg
Lane 6: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-ERK1 antibody [Y72] ab32537 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit Alexa Fluor® 790) ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-ERK1 antibody [Y72] (Anti-ERK1 antibody [Y72] ab32537) at 1/1000 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3: Recombinant Human ERK1 protein (ab43623) (ab43623) at 20 µg
Lane 4: Western blot - Recombinant Human ERK2 protein (Recombinant Human ERK2 protein ab43625) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-ERK2 antibody [E460] ab32081 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-ERK2 antibody [E460] (Anti-ERK2 antibody [E460] ab32081) at 1/1000 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3: 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 20 µg
Lane 4: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg
Lane 5: Recombinant Human ERK1 protein (ab43623) (ab43623) at 20 µg
Lane 6: Western blot - Recombinant Human ERK2 protein (Recombinant Human ERK2 protein ab43625) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Cross-reactivity of the polyclonal secondary antibody Goat Anti-Rabbit IgG H&L ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.
For the batch tested, Goat Anti-Rabbit IgG H&L ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.
This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).
Cross-reactivity of Goat anti-Rabbit IgG H&L (Goat Anti-Rabbit IgG H&L ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (Donkey Anti-Goat IgG H&L (HRP) ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
This data was developed using the unconjugated antibody (Goat Anti-Rabbit IgG H&L ab182016).
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 overnight at 4°C.
Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623) at 1/1000 dilution
Lane 1: Brain (Mouse) Tissue Lysate at 20 µg
Lane 2: Brain (Rat) Tissue Lysate at 20 µg
Lane 3: Spinal Cord (Mouse) Tissue Lysate at 20 µg
Lane 4: Spinal Cord (Rat) Tissue Lysate at 20 µg
Lane 5: PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor® 790 (ab175781) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) at 1/1000 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 4: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 5: NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 6: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Histone H2B (formyl K108) antibody ab177168 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Histone H2B (formyl K108) antibody (Anti-Histone H2B (formyl K108) antibody ab177168) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa, 37 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-Caspase-3 antibody [31A1067] ab13585 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Anti-MDC1 antibody (ab13858) at 1 µg/mL
Lane 1: Hela whole cell lysate (Staurosporine treated, 2uM/4hr) at 20 µg
Lane 2: Hela whole cell lysate (untreated control) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa, 28 kDa, 29 kDa, 31 kDa, 50 kDa
Observed band size: 17 kDa, 19 kDa, 32 kDa, 36 kDa, 43 kDa
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