JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175781

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Be the first to review this product! Submit a review

|

(24 Publications)

Suitable for WB. Cited in 24 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

17 Images
Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32537 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit Alexa Fluor® 790) ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-ERK1 antibody [Y72] (<a href='/en-us/products/primary-antibodies/erk1-antibody-y72-ab32537'>ab32537</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 20 µg

Lane 4:

Western blot - Recombinant Human ERK2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-erk2-protein-ab43625'>ab43625</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 44 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Secondary antibody - anti-rabbit Alexa Fluor 790, ab175781.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-ep1041y-ab52971'>ab52971</a>) at 20 µg

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa,140 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-loading-control-ab18251'>ab18251</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52866 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor® 790 (ab175781) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-ep1332y-loading-control-ab52866'>ab52866</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-loading-control-ab8227'>ab8227</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) Whole Cell Lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor® 790 (ab175781) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-loading-control-ab18251'>ab18251</a>) at 0.5 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney cell line) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Caco-2 (Human colonic carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 27 kDa,50 kDa

Observed band size: 44 kDa,52 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52623 overnight at 4°C.

Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/beta-iii-tubulin-antibody-ep1569y-neuronal-marker-ab52623'>ab52623</a>) at 1/1000 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 2:

Brain (Rat) Tissue Lysate at 20 µg

Lane 3:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Lane 4:

Spinal Cord (Rat) Tissue Lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

This data was developed using the unconjugated antibody (ab182016).

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

This data was developed using the unconjugated antibody (ab182016).

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32081 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/erk2-antibody-e460-ab32081'>ab32081</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 20 µg

Lane 6:

Western blot - Recombinant Human ERK2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-erk2-protein-ab43625'>ab43625</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab156302 overnight at 4°C. Antibody binding was detected using ab175781 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Secondary antibody - goat anti-rabbit Alexa Fluor® 790 (ab175781).

All lanes:

Western blot - Anti-muscle Actin antibody [EPR8484] - Loading Control (<a href='/en-us/products/primary-antibodies/muscle-actin-antibody-epr8484-loading-control-ab156302'>ab156302</a>) at 1/1000 dilution

Lane 1:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg

Lane 6:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody [EP1331Y] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-ep1331y-loading-control-ab52901'>ab52901</a>) at 1/20000 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 2:

Brain (Rat) Tissue Lysate at 20 µg

Lane 3:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Lane 4:

Spinal Cord (Rat) Tissue Lysate at 20 µg

Lane 5:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 52 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab175007 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-asymmetric-di-methyl-r2-antibody-chip-grade-ab175007'>ab175007</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 14 kDa,15 kDa,36 kDa,41 kDa,49 kDa

Observed band size: 17 kDa,18 kDa,37 kDa,42 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177138 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Histone H2B (formyl K5) antibody (<a href='/en-us/products/primary-antibodies/histone-h2b-formyl-k5-antibody-ab177138'>ab177138</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

Lane 3:

NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177168 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Histone H2B (formyl K108) antibody (<a href='/en-us/products/primary-antibodies/histone-h2b-formyl-k108-antibody-ab177168'>ab177168</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa,37 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab13585 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Anti-MDC1 antibody (<a href='/en-us/products/unavailable/mdc1-antibody-ab13858'>ab13858</a>) at 1 µg/mL

Lane 1:

Hela whole cell lysate (Staurosporine treated, 2uM/4hr) at 20 µg

Lane 2:

Hela whole cell lysate (untreated control) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 22 kDa,28 kDa,29 kDa,31 kDa,50 kDa

Observed band size: 17 kDa,19 kDa,32 kDa,36 kDa,43 kDa

false

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)
  • WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52971).

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Secondary antibody -anti-rabbit Alexa Fluor 790, ab175781

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-ep1041y-bsa-and-azide-free-ab192456'>ab192456</a>) at 20 µg

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa,140 kDa

false

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 790

Excitation/Emission

Ex: 782nm, Em: 805nm

Applications

WB

applications

Clonality

Polyclonal

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }
style
left-column

You may be interested in:

AB175775

Goat Anti-Mouse IgG H&L (Alexa Fluor® 680)

0

0 Reviews

View product
AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

5

5 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790), ab175781 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790), ab175781 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Rabbit IgG
style
left-column

Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Animal nutrition (Zhongguo xu mu shou yi xue hui) 16:34-44 PubMed38131029

2023

Mulberry leaf supplementation inhibits skatole deposition by regulating gut microbiota and upregulating liver cytochrome P450 1A1 expression in finishing pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Sun,Xiaoming Men,Tianbao Lin,Bo Deng,Shi Zhong,Jinxi Huo,Kaipeng Qin,Zhiqiang Lv,Ziwei Xu,Yougui Li

Science advances 9:eadi4148 PubMed37624885

2023

Structural basis of telomeric nucleosome recognition by shelterin factor TRF1.

Applications

Unspecified application

Species

Unspecified reactive species

Hongmiao Hu,Anne-Marie M van Roon,George E Ghanim,Bilal Ahsan,Abraham O Oluwole,Sew-Yeu Peak-Chew,Carol V Robinson,Thi Hoang Duong Nguyen

Neuroreport 34:348-356 PubMed36966805

2023

Neuroprotective effects of different doses of Maresin1 pretreatment in aged rats after anesthesia/surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuhua Li,Xu Han,Yubo Gao,Shaling Tang,Yanfang Yang,Chun Zhang,Xinli Ni

Food science & nutrition 11:493-503 PubMed36655066

2023

Alcohol extracts of Chinese bayberry branch induce S-phase arrest and apoptosis in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zheng,Zheping Yu,Yougui Li,Shi Zhong,Yuqing Sun,Li Sun,Xiliang Zheng,Xingjiang Qi,Shuwen Zhang

Microbiology spectrum 10:e0294922 PubMed36377917

2022

Inhibition of the Type III Secretion System of Salmonella enterica Serovar Typhimurium via Treatment with Fraxetin.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjia Shi,Zeyu Sun,Yang Liu,Jingyan Shu,Yong Zhang,Qianghua Lv,Jianfeng Wang,Xuming Deng,Hongtao Liu,Jiazhang Qiu

Journal of neuroinflammation 19:253 PubMed36217178

2022

Promoted CD4 T cell-derived IFN-γ/IL-10 by photobiomodulation therapy modulates neurogenesis to ameliorate cognitive deficits in APP/PS1 and 3xTg-AD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Wu,Qi Shen,Haocai Chang,Junyu Li,Da Xing

Frontiers in cellular and infection microbiology 12:967149 PubMed36176578

2022

Harmine, an inhibitor of the type III secretion system of serovar Typhimurium.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjia Shi,Xindi Chen,Jingyan Shu,Yang Liu,Yong Zhang,Qianghua Lv,Jianfeng Wang,Xuming Deng,Hongtao Liu,Jiazhang Qiu

Cell death discovery 8:228 PubMed35468892

2022

LINC00858 promotes colon cancer progression through activation of STAT3/5 signaling by recruiting transcription factor RAD21 to upregulate PCNP.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xu,Kun Wu,Jin Shi,Lindong Ji,Xudong Song,Guoquan Tao,Shutao Zheng,Li Zhang,Baofei Jiang

Open medicine (Warsaw, Poland) 17:566-576 PubMed35415247

2022

hsa-miR-340-5p inhibits epithelial-mesenchymal transition in endometriosis by targeting MAP3K2 and inactivating MAPK/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yiting Wan,Jiami Huang,Yanhua Song,Cancan Gu,Jueying Kong,Ling Zuo,Jing Chen

Bioengineered 13:6942-6954 PubMed35249453

2022

MicroRNA-193b-3p reduces oxidative stress and mitochondrial damage in rats with cerebral ischemia-reperfusion injury via the seven in absentia homolog 1/Jun N-terminal kinase pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tianye Yang,Jiajun Wu,Kui Ge,Fanlin Wang,Jingxian Fan
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Secondary Antibodies

AB6717

Goat Anti-Rabbit IgG H&L (FITC)

secondary-antibodies

goat-rabbit-igg-h-l-fitc-ab6717

5

(2 reviews)

Alternative Version
Secondary Antibodies

AB175773

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-680-ab175773

0

(0 reviews)

Alternative Version
Secondary Antibodies

AB175471

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-568-ab175471

5

(1 reviews)

Alternative Version
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-488-ab150077

5

(20 reviews)

Alternative Version
Secondary Antibodies

AB150078

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-555-ab150078

5

(2 reviews)

Alternative Version
Secondary Antibodies

AB150079

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-647-ab150079

3

(1 reviews)

Alternative Version
Secondary Antibodies

AB150080

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-594-ab150080

5

(3 reviews)

Alternative Version
Secondary Antibodies

AB182016

Goat Anti-Rabbit IgG H&L

secondary-antibodies

goat-rabbit-igg-h-l-ab182016

0

(0 reviews)

Alternative Version
Secondary Antibodies

AB207995

Goat Anti-Rabbit IgG H&L (Biotin)

secondary-antibodies

goat-rabbit-igg-h-l-biotin-ab207995

0

(0 reviews)

Alternative Version
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

secondary-antibodies

goat-rabbit-igg-h-l-hrp-ab205718

5

(8 reviews)

Alternative Version
Secondary Antibodies

AB175652

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

secondary-antibodies

goat-rabbit-igg-h-l-alexa-fluor-405-ab175652

5

(2 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com