Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

17 Images

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab156302 overnight at 4°C. Antibody binding was detected using ab175781 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Secondary antibody - goat anti-rabbit Alexa Fluor^® 790 (ab175781).

All lanes:

Western blot - Anti-muscle Actin antibody [EPR8484] - Loading Control (<a href='/en-us/products/primary-antibodies/muscle-actin-antibody-epr8484-loading-control-ab156302'>ab156302</a>) at 1/1000 dilution

Lane 1:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg

Lane 6:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab13585 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Anti-MDC1 antibody (<a href='/en-us/products/unavailable/mdc1-antibody-ab13858'>ab13858</a>) at 1 µg/mL

Lane 1:

Hela whole cell lysate (Staurosporine treated, 2uM/4hr) at 20 µg

Lane 2:

Hela whole cell lysate (untreated control) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 22 kDa,28 kDa,29 kDa,31 kDa,50 kDa

Observed band size: 17 kDa,19 kDa,32 kDa,36 kDa,43 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32537 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit Alexa Fluor^® 790) ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-ERK1 antibody [Y72] (<a href='/en-us/products/primary-antibodies/erk1-antibody-y72-ab32537'>ab32537</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 20 µg

Lane 4:

Western blot - Recombinant Human ERK2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-erk2-protein-ab43625'>ab43625</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 44 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor^® 790 (ab175781) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-loading-control-ab18251'>ab18251</a>) at 0.5 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney cell line) whole cell lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Caco-2 (Human colonic carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 27 kDa,50 kDa

Observed band size: 44 kDa,52 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Secondary antibody - anti-rabbit Alexa Fluor 790, ab175781.

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-ep1041y-ab52971'>ab52971</a>) at 20 µg

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa,140 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab18251 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-loading-control-ab18251'>ab18251</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52866 overnight at 4°C. Antibody binding was detected using Anti-Rabbit Alexa Fluor® 790 (ab175781) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-ep1332y-loading-control-ab52866'>ab52866</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-loading-control-ab8227'>ab8227</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) Whole Cell Lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32081 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/erk2-antibody-e460-ab32081'>ab32081</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 20 µg

Lane 6:

Western blot - Recombinant Human ERK2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-erk2-protein-ab43625'>ab43625</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52971).

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Secondary antibody -anti-rabbit Alexa Fluor 790, ab175781

All lanes:

Western blot - Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/integrin-beta-1-antibody-ep1041y-bsa-and-azide-free-ab192456'>ab192456</a>) at 20 µg

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 20 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa,140 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52623 overnight at 4°C.

Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/beta-iii-tubulin-antibody-ep1569y-neuronal-marker-ab52623'>ab52623</a>) at 1/1000 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 2:

Brain (Rat) Tissue Lysate at 20 µg

Lane 3:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Lane 4:

Spinal Cord (Rat) Tissue Lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody [EP1331Y] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-ep1331y-loading-control-ab52901'>ab52901</a>) at 1/20000 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 2:

Brain (Rat) Tissue Lysate at 20 µg

Lane 3:

Spinal Cord (Mouse) Tissue Lysate at 20 µg

Lane 4:

Spinal Cord (Rat) Tissue Lysate at 20 µg

Lane 5:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 52 kDa

false

• ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

This data was developed using the unconjugated antibody (ab182016).

• ELISA

Lab

ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 μl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

This data was developed using the unconjugated antibody (ab182016).

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab175007 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-asymmetric-di-methyl-r2-antibody-chip-grade-ab175007'>ab175007</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 14 kDa,15 kDa,36 kDa,41 kDa,49 kDa

Observed band size: 17 kDa,18 kDa,37 kDa,42 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177138 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Histone H2B (formyl K5) antibody (<a href='/en-us/products/primary-antibodies/histone-h2b-formyl-k5-antibody-ab177138'>ab177138</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

Lane 3:

NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (AB175781)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177168 overnight at 4°C. Antibody binding was detected using ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Histone H2B (formyl K108) antibody (<a href='/en-us/products/primary-antibodies/histone-h2b-formyl-k108-antibody-ab177168'>ab177168</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa,37 kDa

false