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Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

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Images

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (AB186697), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Horse, Chicken, Cow, Human, Pig
Conjugation
Alexa Fluor® 790
Excitation/Emission
Ex: 782nm, Em: 805nm
Applications
WB
Clonality
Polyclonal

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
1/10000
Notes

-

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Recommended products

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

Key facts

Description
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Horse, Chicken, Cow, Human, Pig
Conjugation
Alexa Fluor® 790
Excitation/Emission
Ex: 782nm, Em: 805nm
Applications
WB
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed, ab186697 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed, ab186697 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (ab186697), expandable thumbnail

    Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (ab186697)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-beta Tubulin antibody - Loading Control ab6046 overnight at 4°C. Antibody binding was detected using ab186697 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (Anti-beta Tubulin antibody - Loading Control ab6046) at 1 µg/mL

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (ab186697) at 1/10000 dilution

    Predicted band size: 36 kDa

    Observed band size: 50 kDa

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com