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AB186697

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed

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(8 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

1 Images
Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (AB186697)
  • WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (AB186697)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab186697 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (ab186697) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 790

Excitation/Emission

Ex: 782nm, Em: 805nm

Applications

WB

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed, ab186697 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed, ab186697 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109808 PubMed38741710

2024

Mitochondrial F0F1-ATP synthase governs the induction of mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Charlène Lhuissier,Valérie Desquiret-Dumas,Anaïs Girona,Jennifer Alban,Justine Faure,Julien Cassereau,Philippe Codron,Guy Lenaers,Olivier R Baris,Naïg Gueguen,Arnaud Chevrollier

Communications chemistry 6:95 PubMed37202473

2023

Stereochemical engineering yields a multifunctional peptide macrocycle inhibitor of Akt2 by fine-tuning macrocycle-cell membrane interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Arundhati Nag,Amirhossein Mafi,Samir Das,Mary Beth Yu,Belen Alvarez-Villalonga,Soo-Kyung Kim,Yapeng Su,William A Goddard,James R Heath

Communications biology 6:226 PubMed36854740

2023

Abnormal proliferation of gut mycobiota contributes to the aggravation of Type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Li Bao,Ying Zhang,Guoying Zhang,Dechun Jiang,Dan Yan

Diabetes, metabolic syndrome and obesity : targets and therapy 15:3511-3517 PubMed36411788

2022

Lysophosphatidylcholine (17:0) Improves HFD-Induced Hyperglycemia & Insulin Resistance: A Mechanistic Mice Model Study.

Applications

Unspecified application

Species

Unspecified reactive species

Li Bao,Ying Zhang,Sichao Yan,Dan Yan,Dechun Jiang

ACS omega 6:17276-17288 PubMed34278114

2021

Network Pharmacology-Based Dissection of the Active Ingredients and Protective Mechanism of the and Herb Pair against Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Yang,Wen-Xiao Wang,Yu-Xi Huang,Shi-Jun Yue,Bai-Yang Zhang,Huan Gao,Lei Zhang,Dan Yan,Yu-Ping Tang

Scientific reports 10:2040 PubMed32029775

2020

Renoprotective effects of Gushen Jiedu capsule on diabetic nephropathy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Zhirui Yang,Yidan Zhao,Xinyu Yang,Xintong Meng,Juan Liu,Yi Liu,Can Yan,Dan Yan

Frontiers in physiology 10:1251 PubMed31632293

2019

Quantum Blue Reduces the Severity of Woody Breast Myopathy via Modulation of Oxygen Homeostasis-Related Genes in Broiler Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Greene,Joshua Flees,Sina Dadgar,Barbara Mallmann,Sara Orlowski,Ahmed Dhamad,Samuel Rochell,Michael Kidd,Caroline Laurendon,Hayley Whitfield,Charles Brearley,Narasimhan Rajaram,Carrie Walk,Sami Dridi

American journal of physiology. Endocrinology and metabolism 316:E73-E85 PubMed30422704

2018

Berberine alleviates insulin resistance by reducing peripheral branched-chain amino acids.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Jun Yue,Juan Liu,Ai-Ting Wang,Xin-Tong Meng,Zhi-Rui Yang,Cheng Peng,Hua-Shi Guan,Chang-Yun Wang,Dan Yan
View all publications

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