Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) is a secondary antibody that is suitable for Western blot, IHC, ICC/IF, ELISA and more.
- Cited in over 50 publications
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- Frontiers in pharmacology 14:13353052024Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUma Maheswara Rao Dindi,Sameer Al-Ghamdi,Naif Abdurhman Alrudian,Salman Bin Dayel,Abdulwahab Ali Abuderman,Mohammed Saad Alqahtani,Nasraddin Othman Bahakim,Thiyagarajan Ramesh,Ravikumar VilwanathanPubMed 38235110
- Small (Weinheim an der Bergstrasse, Germany) 19:e22077312023Duplex Electrochemical Microfluidic Sensor for COVID-19 Antibody Detection: Natural versus Vaccine-Induced Humoral Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincenzo Mazzaracchio,Mauricio Rios Maciel,Tatiana Porto Santos,Kazumi Toda-Peters,Amy Q ShenPubMed 36916701
- Scientific reports 13:40362023Novel long non-coding RNAs associated with inflammation and macrophage activation in human.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvisankar Chini,Prarthana Guha,Venkat S Malladi,Zibiao Guo,Subhrangsu S MandalPubMed 36899011
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 275:1113402022Cardiovascular oxygen transport and peripheral oxygen extraction capacity contribute to acute heat tolerance in European seabass.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatja Anttila,Florian Mauduit,Mirella Kanerva,Miriam Götting,Mikko Nikinmaa,Guy ClaireauxPubMed 36347467
- Molecules (Basel, Switzerland) 27:2022Effect of Sex Steroids and PGF on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early-Mid Pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Jamioł,Magdalena Sozoniuk,Jacek Wawrzykowski,Marta KankoferPubMed 36364246
- Frontiers in pharmacology 13:9676232022Chaihu Shugan powder alleviates liver inflammation and hepatic steatosis in NAFLD mice: A network pharmacology study and experimental validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSisi Lei,Shuai Zhao,Xiaoyan Huang,Yuchao Feng,Zhishang Li,Li Chen,Peiying Huang,Hansu Guan,Haobo Zhang,Qihua Wu,Bojun ChenPubMed 36172180
- Analytical chemistry 94:9297-93052022Simultaneous Multiplexed Imaging of Biomolecules in Transgenic Mouse Brain Tissues Using Mass Spectrometry Imaging: A Multi-omic Approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinh-Uyen Thi Le,Hyun Kyong Shon,Hong-Phuong Nguyen,Chul-Ho Lee,Kyoung-Shim Kim,Hee-Kyung Na,Tae Geol LeePubMed 35696262
- Acta physiologica (Oxford, England) 234:e137952022Diet-induced maternal obesity impacts feto-placental growth and induces sex-specific alterations in placental morphology, mitochondrial bioenergetics, dynamics, lipid metabolism and oxidative stress in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTina Napso,Samantha C Lean,Minhui Lu,Emily J Mort,Michelle Desforges,Ali Moghimi,Beverly Bartels,Tatiana El-Bacha,Abigail L Fowden,Emily J Camm,Amanda N Sferruzzi-PerriPubMed 35114078
- Science advances 7:eabj34002021Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Seok Lee,Sung-Bae Lee,Dong-Woon Kim,Nara Shin,Seon-Ju Jeong,Chae-Ha Yang,Chang-Gue SonPubMed 34739315
- Metabolites 11:2021Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyoka Hoshi,Hiromi Ito,Eriko Abe,Takashi J Fuwa,Mayumi Kanno,Yuta Murakami,Mitsunari Abe,Takenobu Murakami,Akioh Yoshihara,Yoshikazu Ugawa,Takashi Saito,Takaomi C Saido,Kana Matsumoto,Yoshiki Yamaguchi,Katsutoshi Furukawa,Hiroyuki Arai,Mitsuyasu Kanai,Masakazu Miyajima,Hajime Arai,Norihiro Ogawa,Hiroyasu Akatsu,Yoshio Hashizume,Hiroaki Tateno,Takashi Honda,Yasuhiro HashimotoPubMed 34564432
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- Dot, ICC, IHC-Fr, IHC-P, WB, ICC/IF, ELISA
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000 - 1/4000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200 - 1/1000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/3000 - 1/25000 | Notes - |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
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Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) is a secondary antibody that is suitable for Western blot, IHC, ICC/IF, ELISA and more.
- Cited in over 50 publications
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- Dot, ICC, IHC-Fr, IHC-P, WB, ICC/IF, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification
- Purification notes
Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody reacts with the heavy and light chains of Rabbit IgG
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5 product images
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
ab6722 was used at dilution 1/10000 with the primary antibody Anti-Catalase antibody - Peroxisome Marker ab16731 in WB. See the review on ab16731.
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 μl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Dot Blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Dot Blot of ab6722.Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Lane 1: Rabbit IgG.Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
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