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AB6722

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

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(51 Publications)

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) is a secondary antibody that is suitable for Western blot, IHC, ICC/IF, ELISA and more.

- Cited in over 50 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

5 Images
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.

Lane M : 3 μl Molecular ladder.

Lane 1 : Rabbit IgG whole molecule.

Lane 2 : Rabbit IgG F(ab) Fragment.

Lane 3 : Rabbit IgG F(c) Fragment.

Lane 4 : Rabbit IgM Whole Molecule.

Lane 5 : Normal Rabbit Serum.

All samples were reduced. Load : 50 ng per lane. Blocked for 30 min at RT.

Primary Antibody : Anti-Rabbit IgG (H&L) (GOAT) Antibody 1 : 1,000 for 60 min at RT. Secondary antibody : Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1 : 40,000 for 30 min at RT.

Predicted/Obsevered Size : 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.

Rabbit F(c) migrates slightly higher.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

false

ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)
  • ELISA

Unknown

ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

ab6722 was used with the primary antibody ab2594 in ELISA. See Abreview on ab2594.

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)
  • WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

ab6722 was used at dilution 1/10000 with the primary antibody ab16731 in WB. See the review on ab16731.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

false

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Lane 1 : Rabbit IgG.

Load : 100 ng per lane.

Secondary antibody : ab6722 used at 1 : 1,000 for 60 min at RT. Blocked for 30 min at RT.

Predicted/Observed size : 55 and 28 kDa, 55 kDa for Rabbit IgG.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

false

Dot Blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)
  • Dot

Supplier Data

Dot Blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Dot Blot of ab6722.

Antigen : Rabbit IgG.

Load : Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.

Secondary antibody : ab6722 used at 1 : 1,000 for 60 min at RT. Blocked for 60 min at RT.

Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alkaline Phosphatase

Excitation/Emission
Applications

ELISA, WB, ICC, IHC-Fr, ICC/IF, IHC-P, Dot

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/4000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/3000 - 1/25000", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1335305 PubMed38235110

2024

Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Uma Maheswara Rao Dindi,Sameer Al-Ghamdi,Naif Abdurhman Alrudian,Salman Bin Dayel,Abdulwahab Ali Abuderman,Mohammed Saad Alqahtani,Nasraddin Othman Bahakim,Thiyagarajan Ramesh,Ravikumar Vilwanathan

Small (Weinheim an der Bergstrasse, Germany) 19:e2207731 PubMed36916701

2023

Duplex Electrochemical Microfluidic Sensor for COVID-19 Antibody Detection: Natural versus Vaccine-Induced Humoral Response.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Mazzaracchio,Mauricio Rios Maciel,Tatiana Porto Santos,Kazumi Toda-Peters,Amy Q Shen

Scientific reports 13:4036 PubMed36899011

2023

Novel long non-coding RNAs associated with inflammation and macrophage activation in human.

Applications

Unspecified application

Species

Unspecified reactive species

Avisankar Chini,Prarthana Guha,Venkat S Malladi,Zibiao Guo,Subhrangsu S Mandal

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 275:111340 PubMed36347467

2022

Cardiovascular oxygen transport and peripheral oxygen extraction capacity contribute to acute heat tolerance in European seabass.

Applications

Unspecified application

Species

Unspecified reactive species

Katja Anttila,Florian Mauduit,Mirella Kanerva,Miriam Götting,Mikko Nikinmaa,Guy Claireaux

Molecules (Basel, Switzerland) 27: PubMed36364246

2022

Effect of Sex Steroids and PGF on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early-Mid Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Jamioł,Magdalena Sozoniuk,Jacek Wawrzykowski,Marta Kankofer

Frontiers in pharmacology 13:967623 PubMed36172180

2022

Chaihu Shugan powder alleviates liver inflammation and hepatic steatosis in NAFLD mice: A network pharmacology study and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Lei,Shuai Zhao,Xiaoyan Huang,Yuchao Feng,Zhishang Li,Li Chen,Peiying Huang,Hansu Guan,Haobo Zhang,Qihua Wu,Bojun Chen

Analytical chemistry 94:9297-9305 PubMed35696262

2022

Simultaneous Multiplexed Imaging of Biomolecules in Transgenic Mouse Brain Tissues Using Mass Spectrometry Imaging: A Multi-omic Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Minh-Uyen Thi Le,Hyun Kyong Shon,Hong-Phuong Nguyen,Chul-Ho Lee,Kyoung-Shim Kim,Hee-Kyung Na,Tae Geol Lee

Acta physiologica (Oxford, England) 234:e13795 PubMed35114078

2022

Diet-induced maternal obesity impacts feto-placental growth and induces sex-specific alterations in placental morphology, mitochondrial bioenergetics, dynamics, lipid metabolism and oxidative stress in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tina Napso,Samantha C Lean,Minhui Lu,Emily J Mort,Michelle Desforges,Ali Moghimi,Beverly Bartels,Tatiana El-Bacha,Abigail L Fowden,Emily J Camm,Amanda N Sferruzzi-Perri

Science advances 7:eabj3400 PubMed34739315

2021

Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Seok Lee,Sung-Bae Lee,Dong-Woon Kim,Nara Shin,Seon-Ju Jeong,Chae-Ha Yang,Chang-Gue Son

Metabolites 11: PubMed34564432

2021

Transferrin Biosynthesized in the Brain Is a Novel Biomarker for Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoka Hoshi,Hiromi Ito,Eriko Abe,Takashi J Fuwa,Mayumi Kanno,Yuta Murakami,Mitsunari Abe,Takenobu Murakami,Akioh Yoshihara,Yoshikazu Ugawa,Takashi Saito,Takaomi C Saido,Kana Matsumoto,Yoshiki Yamaguchi,Katsutoshi Furukawa,Hiroyuki Arai,Mitsuyasu Kanai,Masakazu Miyajima,Hajime Arai,Norihiro Ogawa,Hiroyasu Akatsu,Yoshio Hashizume,Hiroaki Tateno,Takashi Honda,Yasuhiro Hashimoto
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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