AB6722

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

Name: Goat Anti-Rabbit IgG (AP) secondary antibody (ab6722) | Abcam
Brand: Abcam Limited
SKU: AB6722
Price: 265 USD
Availability: InStock

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(57 Publications)

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) is a secondary antibody that is suitable for Western blot, IHC, ICC/IF, ELISA and more.

- Cited in over 50 publications

5 Images

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.

Lane M : 3 μl Molecular ladder.

Lane 1 : Rabbit IgG whole molecule.

Lane 2 : Rabbit IgG F(ab) Fragment.

Lane 3 : Rabbit IgG F(c) Fragment.

Lane 4 : Rabbit IgM Whole Molecule.

Lane 5 : Normal Rabbit Serum.

All samples were reduced. Load : 50 ng per lane. Blocked for 30 min at RT.

Primary Antibody : Anti-Rabbit IgG (H&L) (GOAT) Antibody 1 : 1,000 for 60 min at RT. Secondary antibody : Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1 : 40,000 for 30 min at RT.

Predicted/Obsevered Size : 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.

Rabbit F(c) migrates slightly higher.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

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ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

ELISA

Unknown

ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

ab6722 was used with the primary antibody ab2594 in ELISA. See Abreview on ab2594.

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

ab6722 was used at dilution 1/10000 with the primary antibody ab16731 in WB. See the review on ab16731.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

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Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Lane 1 : Rabbit IgG.

Load : 100 ng per lane.

Secondary antibody : ab6722 used at 1 : 1,000 for 60 min at RT. Blocked for 30 min at RT.

Predicted/Observed size : 55 and 28 kDa, 55 kDa for Rabbit IgG.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

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Supplier Data

Dot Blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Dot Blot of ab6722.

Antigen : Rabbit IgG.

Load : Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.

Secondary antibody : ab6722 used at 1 : 1,000 for 60 min at RT. Blocked for 60 min at RT.

Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

Dot, WB, IHC-P, ICC, IHC-Fr, ELISA, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/4000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/3000 - 1/25000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 22:16 PubMed40295933

2025

An ALS assembly modulator signature in peripheral blood mononuclear cells: implications for ALS pathophysiology, therapeutics, and diagnostics.

Applications

Unspecified application

Species

Unspecified reactive species

Shao Feng Yu,Maya Michon,Anuradha F Lingappa,Kumar Paulvannan,Dennis Solas,Kim Staats,Justin Ichida,Debendranath Dey,Jeffrey Rosenfeld,Vishwanath R Lingappa

NPJ precision oncology 9:64 PubMed40055530

2025

Role of GPX3+ astrocytes in breast cancer brain metastasis activated by circulating tumor cell exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Huang,Gongwen Xu,Qianqian Cao,Sheng Li,Hao Li,Xiaonan Zhang,Xiaomei Li

Environmental health perspectives 132:107004 PubMed39412896

2024

Lead Acetate Exposure and Cerebral Amyloid Accumulation: Mechanistic Evaluations in APP/PS1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huiying Gu,Luqing L Liu,Alanna Wu,Yongqi Yu,Uzay Emir,Stephen J Sawiak,Paul R Territo,Matin R Farlow,Wei Zheng,Yansheng Du

Journal of experimental botany 76:411-432 PubMed39377268

2024

The MADS-box gene XAANTAL1 participates in Arabidopsis thaliana primary root growth and columella stem cell patterns in response to ROS, via direct regulation of PEROXIDASE 28 and RETINOBLASTOMA-RELATED genes.

Applications

Unspecified application

Species

Unspecified reactive species

Estephania Zluhan-Martínez,Claudio A Castañón-Suárez,Mario A Gutiérrez-Rodríguez,Fernando Lledías,Tao Zhang,Jesús T Peng,Jazz Dickinson,Diana Belén Sánchez Rodríguez,María de la Paz Sánchez,Berenice García-Ponce,Elena R Álvarez-Buylla,Adriana Garay-Arroyo

The Journal of cell biology 223: PubMed38241019

2024

Mechanism of exportin retention in the cell nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Larisa E Kapinos,Joanna Kalita,Elena Kassianidou,Chantal Rencurel,Roderick Y H Lim

Frontiers in pharmacology 14:1335305 PubMed38235110

2024

Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Uma Maheswara Rao Dindi,Sameer Al-Ghamdi,Naif Abdurhman Alrudian,Salman Bin Dayel,Abdulwahab Ali Abuderman,Mohammed Saad Alqahtani,Nasraddin Othman Bahakim,Thiyagarajan Ramesh,Ravikumar Vilwanathan

Journal of virology 98:e0162323 PubMed38193692

2024

An HTLV-1 envelope mRNA vaccine is immunogenic and protective in New Zealand rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua J Tu,Emily King,Victoria Maksimova,Susan Smith,Ramon Macias,Xiaogang Cheng,Tanmayee Vegesna,Lianbo Yu,Lee Ratner,Patrick L Green,Stefan Niewiesk,Justin M Richner,Amanda R Panfil

Small (Weinheim an der Bergstrasse, Germany) 19:e2207731 PubMed36916701

2023

Duplex Electrochemical Microfluidic Sensor for COVID-19 Antibody Detection: Natural versus Vaccine-Induced Humoral Response.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Mazzaracchio,Mauricio Rios Maciel,Tatiana Porto Santos,Kazumi Toda-Peters,Amy Q Shen

Scientific reports 13:4036 PubMed36899011

2023

Novel long non-coding RNAs associated with inflammation and macrophage activation in human.

Applications

Unspecified application

Species

Unspecified reactive species

Avisankar Chini,Prarthana Guha,Venkat S Malladi,Zibiao Guo,Subhrangsu S Mandal

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 275:111340 PubMed36347467

2022

Cardiovascular oxygen transport and peripheral oxygen extraction capacity contribute to acute heat tolerance in European seabass.

Applications

Unspecified application

Species

Unspecified reactive species

Katja Anttila,Florian Mauduit,Mirella Kanerva,Miriam Götting,Mikko Nikinmaa,Guy Claireaux

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Dot Blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB6722)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

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Primary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (57)

Product promise