Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB97048)

All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) whole cell lysate

Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/50000 dilution

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)

true

Exposure time: 4min

• WB

PubMed

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB97048)

RSV mutants show expected patterns of G, F, and M2-1 expression.

Western blotting of Vero cell lysates infected with RSV ΔG, ΔG C-term, and wild type (MSA1). (Figure A) Detection of G using anti-G polyclonal antibody. (Figure B) Detection of F using anti-F antibody. (Figure C) Detection of M2-1 using anti-M2-1 antibody. Each Figure shows samples that were run on the same gel and irrelevant lanes are covered with white rectangles. Molecular weights in kD are shown in Figure A.

Cell lysates from virus cultures grown for 6 days on Vero cells were subjected to SDS-PAGE, transferred to membranes and probed with a proprietary anti-RSV F antibody or anti-M2-1 antibody (Abcam, ab94805), and incubated with a secondary goat anti-mouse alkaline phosphatase conjugate. Anti-G western blot was done with rabbit anti-RSV-G polyclonal and incubated with goat anti-rabbit secondary antibody (Abcam, ab97048). Bands were visualized using BCIP/NBT. The molecular weight marker used was Precision Plus Protein Dual Color Standards.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)

false

Jeong et al PLoS One. 2015 Jun 24;10(6):e0130517. doi: 10.1371/journal.pone.0130517. eCollection 2015. File S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/