Suitable for ICC, WB, IHC-P, ELISA. Cited in 42 publications.
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- International journal of molecular sciences 25:2024Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone?Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwa Tomaszewska,Monika Hułas-Stasiak,Piotr Dobrowolski,Małgorzata Świątkiewicz,Siemowit Muszyński,Agnieszka Tomczyk-Warunek,Tomasz Blicharski,Janine Donaldson,Marcin B Arciszewski,Michał Świetlicki,Iwona Puzio,Joanna BoniorPubMed 38396667
- Plants (Basel, Switzerland) 12:2023Isolation and Characterization of Extracellular Vesicles from Cell Culture and Investigation of the Specificities of Their Biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulia Yugay,Zhargalma Tsydeneshieva,Tatiana Rusapetova,Olga Grischenko,Anastasia Mironova,Dmitry Bulgakov,Vladimir Silant'ev,Galina Tchernoded,Victor Bulgakov,Yury ShkrylPubMed 37896067
- Nature medicine 29:2866-28842023Microglia and complement mediate early corticostriatal synapse loss and cognitive dysfunction in Huntington's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel K Wilton,Kevin Mastro,Molly D Heller,Frederick W Gergits,Carly Rose Willing,Jaclyn B Fahey,Arnaud Frouin,Anthony Daggett,Xiaofeng Gu,Yejin A Kim,Richard L M Faull,Suman Jayadev,Ted Yednock,X William Yang,Beth StevensPubMed 37814059
- Current issues in molecular biology 45:7827-78412023T-Lymphocytes Activated by Dendritic Cells Loaded by Tumor-Derived Vesicles Decrease Viability of Melanoma Cells In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvan Yurevich Filin,Yuriy Pavlovich Mayasin,Chulpan Bulatovna Kharisova,Anna Valerevna Gorodilova,Daria Sergeevna Chulpanova,Kristina Viktorovna Kitaeva,Albert Anatolyevich Rizvanov,Valeria Vladimirovna SolovyevaPubMed 37886937
- International journal of molecular sciences 24:2023Prenatal Fumonisin Exposure Impairs Bone Development via Disturbances in the OC/Leptin and RANKL/RANK/OPG Systems in Weaned Rat Offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwa Tomaszewska,Halyna Rudyk,Siemowit Muszyński,Monika Hułas-Stasiak,Norbert Leszczyński,Maria Mielnik-Błaszczak,Janine Donaldson,Piotr DobrowolskiPubMed 37240089
- International journal of molecular sciences 24:2023Repetitive Cerulein-Induced Chronic Pancreatitis in Growing Pigs-A Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwa Tomaszewska,Małgorzata Świątkiewicz,Siemowit Muszyński,Janine Donaldson,Katarzyna Ropka-Molik,Marcin B Arciszewski,Maciej Murawski,Tomasz Schwarz,Piotr Dobrowolski,Sylwia Szymańczyk,Sławomir Dresler,Joanna BoniorPubMed 37175426
- Microbiology spectrum 11:e03103222022Influence of Streptococcus pneumoniae Within-Strain Population Diversity on Virulence and Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura C Jacques,Angharad E Green,Thomas E Barton,Murielle Baltazar,Julia Aleksandrowicz,Rong Xu,Erwan Trochu,Aras Kadioglu,Daniel R NeillPubMed 36507681
- Genetics 222:2022Turning coldspots into hotspots: targeted recruitment of axis protein Hop1 stimulates meiotic recombination in Saccharomyces cerevisiae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnura Shodhan,Martin Xaver,David Wheeler,Michael LichtenPubMed 35876814
- Environmental toxicology 37:1853-18662022LINC00365 inhibited lung adenocarcinoma progression and glycolysis via sponging miR-429/KCTD12 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng-Wei Zhang,Bin Zhou,Yan-Chao Liu,Li-Wei Su,Jie Meng,Shao-Lei Li,Xue-Long WangPubMed 35426242
- Frontiers in bioengineering and biotechnology 10:7974372022Magnetic Nanoparticle-Mediated Orientation of Collagen Hydrogels for Engineering of Tendon-Mimetic Constructs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbigail L Wright,Lucrezia Righelli,T J Broomhall,Hannah C Lamont,Alicia J El HajPubMed 35372293
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- ICC, WB, IHC-P, ELISA
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/5000.00000 - 1/50000.00000 | Notes Chemiluminescent detection |
Application IHC-P | Reactivity Reacts | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/5000.00000 - 1/50000.00000 | Notes Primary |
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
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Suitable for ICC, WB, IHC-P, ELISA. Cited in 42 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- ICC, WB, IHC-P, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non- immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Part of the AbExcel range.
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Jeong et al PLoS One. 2015 Jun 24;10(6):e0130517. doi: 10.1371/journal.pone.0130517. eCollection 2015. File S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)
RSV mutants show expected patterns of G, F, and M2-1 expression.Western blotting of Vero cell lysates infected with RSV ΔG, ΔG C-term, and wild type (MSA1). (Figure A) Detection of G using anti-G polyclonal antibody. (Figure B) Detection of F using anti-F antibody. (Figure C) Detection of M2-1 using anti-M2-1 antibody. Each Figure shows samples that were run on the same gel and irrelevant lanes are covered with white rectangles. Molecular weights in kD are shown in Figure A.
Cell lysates from virus cultures grown for 6 days on Vero cells were subjected to SDS-PAGE, transferred to membranes and probed with a proprietary anti-RSV F antibody or anti-M2-1 antibody (Abcam, Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] ab94805), and incubated with a secondary goat anti-mouse alkaline phosphatase conjugate. Anti-G western blot was done with rabbit anti-RSV-G polyclonal and incubated with goat anti-rabbit secondary antibody (Abcam, ab97048). Bands were visualized using BCIP/NBT. The molecular weight marker used was Precision Plus Protein Dual Color Standards.
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)
All lanes : Anti-beta Actin antibody - Loading Control (Anti-beta Actin antibody ab8227) at 1 μg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) whole cell lysate Lysates/proteins at 10 μg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutesSecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)
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