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Goat Anti-Rabbit IgG H&L (APC) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 660nm. Ideal for fluorescent cell and tissue imaging. Suitable for Western blot, IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 10 publications

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Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Human
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Applications
WB, ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
Clonality
Polyclonal

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
0.1 µg for 106 Cells
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

use at ≤ 0.1 μg/106 cells

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Goat Anti-Rabbit IgG H&L (APC) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 660nm. Ideal for fluorescent cell and tissue imaging. Suitable for Western blot, IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 10 publications

Key facts

Description
Goat Anti-Rabbit IgG H&L (APC) preadsorbed
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Human
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Applications
WB, ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

Reacts with the heavy and light chains of Rabbit IgG, and with the light chains of Rabbit IgM. Goat Anti-Rabbit IgG H&L (Allophycocyanin) preadsorbed shows minimal cross reactivity with Human and Mouse immunoglobulins.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, Sucrose

Purification technique
Affinity purification Immunogen
Purification notes

ab130805 was purified by affinity chromatography on pooled IgG covalently linked to agarose. ab130805 has been cross absorbed with purified Human paraproteins, pooled Mouse sera, and pooled Mouse plasmacytoma/hybridoma proteins.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

This antibody reacts with the heavy and light chains of Rabbit IgG

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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