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Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA.

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Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Human
Conjugation
Beta-galactosidase
Applications
WB, ICC, IHC-Fr, IHC-P, ELISA
Clonality
Polyclonal

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/100 - 1/1000
Notes

-

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Recommended products

Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA.

Key facts

Description
Goat Anti-Rabbit IgG H&L (beta-galactosidase)
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Human
Conjugation
Beta-galactosidase
Applications
WB, ICC, IHC-Fr, IHC-P, ELISA
Clonality
Polyclonal
Pre-adsorbed
No
Specificity

ab136774 does not cross react with Human IgG, A, M.


Beta-galactosidase labelling provides good sensitivity and does not present endogenous activity in mammalian cells. It is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Purification technique
Affinity purification Immunogen
Purification notes

ab136774 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle

Notes

Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.

Product promise

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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