AB97049

Goat Anti-Rabbit IgG H&L (Biotin)

Name: Goat Anti Rabbit (IgG) secondary antibody Biotin (ab97049) | Abcam
Brand: Abcam Limited
SKU: AB97049
Price: 265 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(37 Publications)

Goat Anti-Rabbit IgG H&L (Biotin)(ab97049) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC, ICC/IF, ELISA and more.

- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method
- Proven performance: cited in over 35 publications

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB97049)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB97049)

ab97049 was used at dilution 1 : 500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

Image on the left : isotype control image

This image is courtesy of Dr Kyle Caires

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

IHC-P, WB, ELISA, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody reactivity was detected against non-immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/400000", "notes":"ELISA-Primary" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/400000", "notes":"WB-Chemiluminescent" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1572299 PubMed40538844

2025

Digital spatial profiling identifies phospho-JNK as a biomarker for early risk stratification of aggressive prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Samaneh Eickelschulte,Adam Kaczorowski,Florian Janke,Anja Lisa Riediger,Olga Lazareva,Sarah Böning,Glen Kristiansen,Constantin Schwab,Albrecht Stenzinger,Holger Sültmann,Stefan Duensing,Anette Duensing,Magdalena Görtz

Polymers 16: PubMed39274180

2024

Chitosan siRNA Nanoparticles Produce Significant Non-Toxic Functional Gene Silencing in Kidney Cortices.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamad-Gabriel Alameh,Ashkan Tavakoli Naeini,Garima Dwivedi,Frederic Lesage,Michael D Buschmann,Marc Lavertu

Scientific reports 14:20281 PubMed39217197

2024

Digital spatial profiling identifies the tumor center as a topological niche in prostate cancer characterized by an upregulation of BAD.

Applications

Unspecified application

Species

Unspecified reactive species

Ann-Kathrin Huber,Adam Kaczorowski,Felix Schneider,Sarah Böning,Magdalena Görtz,David Langhoff,Constantin Schwab,Albrecht Stenzinger,Markus Hohenfellner,Anette Duensing,Stefan Duensing

Pediatric research 96:952-963 PubMed38684886

2024

Lithium rescues cultured rat metatarsals from dexamethasone-induced growth failure.

Applications

Unspecified application

Species

Unspecified reactive species

Ondrej Soucek,Ondrej Cinek,Lilly Velentza,Valerij Semjonov,Martin Bezdicka,Farasat Zaman,Lars Sävendahl

Animal reproduction 21:e20230112 PubMed38628494

2024

Expression of luteinizing hormone receptor during development of bovine fetal ovary.

Applications

Unspecified application

Species

Unspecified reactive species

Alan Brunholi Giroto,Marina Platzeck Chaves,Priscila Helena Dos Santos,Patrícia Kubo Fontes,Sarah Gomes Nunes,Thainá Sallum Bacco Manssur,Leonardo Oliveira Mendes,Anthony César de Souza Castilho

Journal of the Endocrine Society 8:bvae009 PubMed38328478

2024

Humanin Treatment Protects Against Venetoclax-Induced Bone Growth Retardation in Cultured Rat Bones.

Applications

Unspecified application

Species

Unspecified reactive species

Lilly Velentza,Malin Wickström,Per Kogner,Claes Ohlsson,Farasat Zaman,Lars Sävendahl

Cancers 15: PubMed38136261

2023

Long-Term Survival of Patients with Stage T1N0M1 Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Schütz,Huan Lin,Adam Kaczorowski,Stefanie Zschäbitz,Dirk Jäger,Albrecht Stenzinger,Anette Duensing,Jürgen Debus,Markus Hohenfellner,Stefan Duensing

Aging and disease 14:2193-2214 PubMed37199590

2023

Healthy Human Fecal Microbiota Transplantation into Mice Attenuates MPTP-Induced Neurotoxicity via AMPK/SOD2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenchao Xie,Mahui Zhang,Yuqi Luo,Dana Jin,Xingfang Guo,Wanlin Yang,Jialing Zheng,Hongfei Zhang,Lu Zhang,Chao Deng,Wenhua Zheng,Eng-King Tan,Kunlin Jin,Shuzhen Zhu,Qing Wang

Journal of translational medicine 21:855 PubMed38012763

2023

METTL3-mediated HOTAIRM1 promotes vasculogenic mimicry icontributionsn glioma via regulating IGFBP2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyi Wu,Nan Wei

Cancers 15: PubMed37894418

2023

Digital Spatial Profiling Identifies the Tumor Periphery as a Highly Active Biological Niche in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Schneider,Adam Kaczorowski,Christina Jurcic,Martina Kirchner,Constantin Schwab,Viktoria Schütz,Magdalena Görtz,Stefanie Zschäbitz,Dirk Jäger,Albrecht Stenzinger,Markus Hohenfellner,Stefan Duensing,Anette Duensing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Rabbit IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB97049)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (37)

Product promise