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Suitable for ELISA, IHC-P, ICC/IF, WB. Cited in 31 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB97049), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Biotin
Applications
ELISA, IHC-P, ICC/IF, WB
Clonality
Polyclonal

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Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
1/20000.00000 - 1/400000.00000
Notes

ELISA-Primary

Application
IHC-P
Reactivity
Reacts
Dilution info
1/500.00000 - 1/5000.00000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/20000.00000 - 1/400000.00000
Notes

WB-Chemiluminescent

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Alternative names

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Suitable for ELISA, IHC-P, ICC/IF, WB. Cited in 31 publications.

Key facts

Description
Goat Anti-Rabbit IgG H&L (Biotin)
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Biotin
Applications
ELISA, IHC-P, ICC/IF, WB
Clonality
Polyclonal
Pre-adsorbed
No
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody reactivity was detected against non-immunoglobulin serum proteins.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

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Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab97049)

    ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

    Image on the left: isotype control image

    This image is courtesy of Dr Kyle Caires

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Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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