AB7089

Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Biotin (ab7089) | Abcam
Brand: Abcam Limited
SKU: AB7089
Price: 295 USD
Availability: InStock

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(7 Publications)

Goat Anti-Rabbit IgG H&L (Biotin)(ab7089) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC and ELISA.

- Preadsorbed to minimize non-specific binding and high background staining
- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method

1 Images

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed (AB7089)

Western blot analysis of ab7089. Secondary antibody : Peroxidase streptavidin secondary antibody at 1/40000 dilution for 30 min at RT.

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab7089) at 1/1000 dilution

All lanes:

Rabbit IgG at 50 ng

Secondary

All lanes:

Peroxidase streptavidin secondary antibody at 1/40000 dilution

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Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human, Mouse

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

WB, Dot, ELISA, IHC-Fr, IM, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/20000 - 1/100000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

ACS chemical biology 17:941-956 PubMed35385646

2022

Panel of Engineered Ubiquitin Variants Targeting the Family of Human Ubiquitin Interacting Motifs.

Applications

Unspecified application

Species

Unspecified reactive species

Gianluca Veggiani,Bradley P Yates,Gregory D Martyn,Noah Manczyk,Alex U Singer,Igor Kurinov,Frank Sicheri,Sachdev S Sidhu

Frontiers in physiology 12:700338 PubMed34385934

2021

Blockage of C-X-C Motif Chemokine Receptor 2 (CXCR2) Suppressed Uric Acid (UA)-Induced Cardiac Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxi Xu,Xu Zheng,Dongxia Wang,Xiaodan Fu,Yida Xing,Yu Liu,Hongjiang Wang,Xiaodan Kong

Oncology reports 46: PubMed34278497

2021

EphA8 inhibits cell apoptosis via AKT signaling and is associated with poor prognosis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Hua Wang,Kan Ni,Changjiang Gu,Jianfei Huang,Jing Chen,Xu-Dong Wang,Qichao Ni

Oncology reports 45:1044-1058 PubMed33650655

2021

Nucleolar and spindle‑associated protein 1 promotes non‑small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Ling,Pengya Wei,Juan Xiao,Bingkui Cen,Hong Wei,Xueping Feng,Guangbin Ye,Songbo Li,Zhongwei Zhang,Wei Liang,Suoyi Huang,Wei Huang

Oncology reports 44:2465-2474 PubMed33125146

2020

Long non‑coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiliang Guan,Yufen Xu,Jufen Zheng

PLoS biology 16:e2006660 PubMed30543635

2018

Direct visualization of single-molecule membrane protein interactions in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Do-Hyeon Kim,Soyeon Park,Dong-Kyun Kim,Min Gyu Jeong,Jungeun Noh,Yonghoon Kwon,Kai Zhou,Nam Ki Lee,Sung Ho Ryu

Nature structural & molecular biology 21:955-961 PubMed25306109

2014

Cross-talking noncoding RNAs contribute to cell-specific neurodegeneration in SCA7.

Applications

Species

Unspecified reactive species

Jennifer Y Tan,Keith W Vance,Miguel A Varela,Tamara Sirey,Lauren M Watson,Helen J Curtis,Martina Marinello,Sandro Alves,Bruno Steinkraus,Sarah Cooper,Tatyana Nesterova,Neil Brockdorff,Tudor Fulga,Alexis Brice,Annie Sittler,Peter L Oliver,Matthew J Wood,Chris P Ponting,Ana C Marques

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed

Western blot - Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed (AB7089)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise