AB97073

Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Biotin (ab97073) | Abcam
Brand: Abcam Limited
SKU: AB97073
Price: 295 USD
Availability: InStock

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(6 Publications)

Suitable for WB, IHC-P, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

ELISA, ICC/IF, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Chemiluminescent)" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Primary)" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:749153 PubMed34858976

2021

Malaria Parasites Hijack Host Receptors From Exosomes to Capture Lipoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Naoyuki Iso-O,Keisuke Komatsuya,Fuyuki Tokumasu,Noriko Isoo,Tomohiro Ishigaki,Hiroshi Yasui,Hiroshi Yotsuyanagi,Masumi Hara,Kiyoshi Kita

Journal of cellular and molecular medicine 25:4455-4465 PubMed33797200

2021

Exosomes from neuronal stem cells may protect the heart from ischaemia/reperfusion injury via JAK1/2 and gp130.

Applications

Unspecified application

Species

Unspecified reactive species

Miroslava Katsur,Zhenhe He,Vladimir Vinokur,Randolph Corteling,Derek M Yellon,Sean M Davidson

Journal of extracellular vesicles 8:1560809 PubMed30651940

2019

Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.

Applications

Unspecified application

Species

Unspecified reactive species

Kaloyan Takov,Derek M Yellon,Sean M Davidson

Journal of extracellular vesicles 6:1388731 PubMed29184625

2017

Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes.

Applications

Unspecified application

Species

Unspecified reactive species

Kaloyan Takov,Derek M Yellon,Sean M Davidson

Molecular carcinogenesis 55:575-85 PubMed25764520

2015

miR-577 inhibits glioblastoma tumor growth via the Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weiguang Zhang,Chen Shen,Chenguang Li,Guang Yang,Huailei Liu,Xin Chen,Dan Zhu,Huichao Zou,Yunbo Zhen,Daming Zhang,Shiguang Zhao

Anatomical record (Hoboken, N.J. : 2007) 296:921-32 PubMed23580480

2013

Novel spatiotemporal glycome changes in the murine placenta during placentation based on BS-I lectin binding patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Charalambous,Katerina Drakou,Stavros Nicolaou,Pantelis Georgiades

View all publications

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Product promise

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Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise