AB6940

Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

Name: Goat Anti Rabbit (IgG) secondary antibody preadsorbed Cy2® (ab6940) | Abcam
Brand: Abcam Limited
SKU: AB6940
Rating: 5 (1 reviews)

(1 Review)

(47 Publications)

Goat Anti-Rabbit IgG H&L (Cy2 ®) preabsorbed (ab6940) is designed to minimize non-specific binding and high background staining. Suitable for Western blot Flow Cytometry, IHC, ICC/IF and ELISA applications.

- Proven performance: cited in over 45 publications.

3 Images

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Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/10000 dilution

false

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with the primary antibody for 1 hour at room temperature.

Incubated with the secondary antibody for 30 minutes at room temperature.

Lane 1:

Rabbit IgG whole molecule at 0.05 µg

Lane 2:

Rabbit IgG F(ab) fragment at 0.05 µg

Lane 3:

Rabbit IgG F(c) fragment at 0.05 µg

Lane 4:

Rabbit IgM whole molecule at 0.05 µg

Lane 5:

Normal rabbit serum at 0.05 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

Lanes 1 - 5:

Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution

false

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with ab6940 for 1 hour at room temperature.

All lanes:

Rabbit IgG at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

false

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Guinea pig, Hamster, Cow, Human, Chicken

Pre-adsorbed

Yes

Conjugation

Cy2®

Excitation/Emission

Ex: 489nm, Em: 505nm

Applications

ICC, IHC-P, Flow Cyt, IHC-Fr, ELISA, WB, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" } } }

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (47)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 16:20417314251326668 PubMed40342587

2025

Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Mercedes Olvera-Valencia,Verónica Garcia-Castillo,Rosalío Ramos-Payan,Maribel Aguilar-Medina,Samuel Trujano-Camacho,Alejandro López-Saavedra,Laurence A Marchat,Cesar López-Camarillo,Ronen Sumagin,Eloy Pérez-Yepez,Carlos Pérez-Plasencia

Communications biology 8:514 PubMed40155473

2025

Defining gastric cancer ecology: the crucial roles of TREM2 macrophages and fibroblasts in tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Cao,Dianshui Sun,Can Tu,Jihua Wang,Runjia Fu,Rumei Gong,Yueying Xiao,Qin Liu,Xiaomei Li

Molecular biology reports 52:302 PubMed40080230

2025

Simultaneous isolation and culture of endothelial colony-forming cells, endothelial cells and vascular smooth muscle cells from human umbilical cords.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Lotus Burger,Steeve Menétrey,Catherine Ponti,Karine Lepigeon,Joanna Sichitiu,Anne-Christine Peyter

Bio-protocol 15:e5181 PubMed39959295

2025

Development and Validation of Mouse Models for Studying Genital Tract Infection Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Zixuan Han,Luying Wang,Xin Sun,Qi Tian,Tianyuan Zhang

World journal of gastroenterology 30:5174-5190 PubMed39735272

2024

N6-methyladenosine-modified long non-coding RNA promotes stemness and sorafenib resistance in hepatocellular carcinoma by upregulating SHOX2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yu,Xiang-Hong Lu,Jin-Song Mu,Jiang-Yun Meng,Jiang-Shan Sun,Hai-Xu Chen,Yang Yan,Ke Meng

Cells 13: PubMed39404412

2024

Laherradurin Inhibits Colorectal Cancer Cell Growth by Induction of Mitochondrial Dysfunction and Autophagy Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Izamary Delgado-Waldo,Svetlana Dokudovskaya,Yahir A Loissell-Baltazar,Eduardo Pérez-Arteaga,Jossimar Coronel-Hernández,Mariano Martínez-Vázquez,Eloy Andrés Pérez-Yépez,Alejandro Lopez-Saavedra,Nadia Jacobo-Herrera,Carlos Pérez Plasencia

The International journal of developmental biology 68:19-24 PubMed38591690

2024

Disrupted odontoblast differentiation and dentin dysplasia in -deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Jiménez-Rojo,Susana de Vega,Gaskon Ibarretxe,Takashi Nakamura,Fernando J Unda

Drug metabolism and disposition: the biological fate of chemicals 51:1230-1237 PubMed37349114

2023

NEAT1_2 and DAZAP1, Paraspeckle Components, Interact with PXR to Negatively Regulate CYP3A4 Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Rei Mitamura,Masataka Nakano,Motoki Isono,Kiamu Kurosawa,Tatsuki Fukami,Miki Nakajima

Theranostics 13:3550-3567 PubMed37441598

2023

Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Chen,Guihui Qin,Yuhong Liu,Moxin Li,Yue Li,Lun-Zhang Guo,Lidong Du,Weiming Zheng,Pei-Chun Wu,Yueh-Hsun Chuang,Xiaoyan Wang,Tzung-Dau Wang,Ja-An Annie Ho,Tzu-Ming Liu

Frontiers in microbiology 14:1142283 PubMed37125189

2023

Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Tianyuan Zhang,Luying Wang,Jingyue Ma,Xin Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (47)

Product promise