Suitable for WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 39 publications.
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- Drug metabolism and disposition: the biological fate of chemicals 51:1230-12372023NEAT1_2 and DAZAP1, Paraspeckle Components, Interact with PXR to Negatively Regulate CYP3A4 Induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRei Mitamura,Masataka Nakano,Motoki Isono,Kiamu Kurosawa,Tatsuki Fukami,Miki NakajimaPubMed 37349114
- Theranostics 13:3550-35672023Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiping Chen,Guihui Qin,Yuhong Liu,Moxin Li,Yue Li,Lun-Zhang Guo,Lidong Du,Weiming Zheng,Pei-Chun Wu,Yueh-Hsun Chuang,Xiaoyan Wang,Tzung-Dau Wang,Ja-An Annie Ho,Tzu-Ming LiuPubMed 37441598
- Frontiers in microbiology 14:11422832023Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Tian,Tianyuan Zhang,Luying Wang,Jingyue Ma,Xin SunPubMed 37125189
- Scientific reports 13:32532023Experimental venous thrombus resolution is driven by IL-6 mediated monocyte actions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea T Obi,Sriganesh B Sharma,Megan A Elfline,Catherine E Luke,Abigail R Dowling,Qing Cai,Andrew S Kimball,Mike Hollinstat,Livia Stanger,Bethany B Moore,Farouc A Jaffer,Peter K HenkePubMed 36828892
- Journal of translational medicine 20:4852022Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuxia Deng,Xiansheng Lu,Chang Hong,Rui Cai,Ping Wang,Le Xiong,Xiaoyu Wang,Qiaoyu Chen,Jie LinPubMed 36274132
- Journal of healthcare engineering 2021:27717122021CircRNA RERE Promotes the Oxidative Stress-Induced Apoptosis and Autophagy of Nucleus Pulposus Cells through the miR-299-5p/Galectin-3 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Wang,Xingchao Zhou,Guorui Luo,Jin Zhang,Min Yang,Chao SongPubMed 34956563
- Experimental and therapeutic medicine 21:672020miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Ren,Ruanzhong Bao,Zhujun Guo,Jin Kai,Chen-Ge Cai,Zhu LiPubMed 33365067
- Cell cycle (Georgetown, Tex.) 19:2530-25372020Lidocaine alleviates cisplatin resistance and inhibits migration of MGC-803/DDP cells through decreasing miR-10b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomin Zhang et. al.PubMed 32892697
- Cancer cell international 20:3352020LncRNA ANRIL/miR-7-5p/TCF4 axis contributes to the progression of T cell acute lymphoblastic leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Li et. al.PubMed 32714094
- Cell communication and signaling : CCS 18:1062020Renal cancer-derived exosomes induce tumor immune tolerance by MDSCs-mediated antigen-specific immunosuppression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingying Gao et. al.PubMed 32641056
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Goat, Horse, Guinea pig, Hamster, Cow, Human, Chicken
- Conjugation
- Cy2®
- Applications
- WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 39 publications.
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Goat, Horse, Guinea pig, Hamster, Cow, Human, Chicken
- Conjugation
- Cy2®
- Applications
- WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Purification technique
- Affinity purification Immunogen
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
Incubated with ab6940 for 1 hour at room temperature.
All lanes: Rabbit IgG at 0.05 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
Incubated with the primary antibody for 1 hour at room temperature.
Incubated with the secondary antibody for 30 minutes at room temperature.
Lane 1: Rabbit IgG whole molecule at 0.05 µg
Lane 2: Rabbit IgG F(ab) fragment at 0.05 µg
Lane 3: Rabbit IgG F(c) fragment at 0.05 µg
Lane 4: Rabbit IgM whole molecule at 0.05 µg
Lane 5: Normal rabbit serum at 0.05 µg
SecondaryLanes 1 - 5: Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution
Lanes 1 - 5: Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution
Performed under reducing conditions.
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
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