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AB6940

Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

5

(1 Review)

|

(39 Publications)

Goat Anti-Rabbit IgG H&L (Cy2 ®) preabsorbed (ab6940) is designed to minimize non-specific binding and high background staining. Suitable for Western blot Flow Cytometry, IHC, ICC/IF and ELISA applications.

- Proven performance: cited in over 35 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with ab6940 for 1 hour at room temperature.

All lanes:

Rabbit IgG at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

false

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with the primary antibody for 1 hour at room temperature.

Incubated with the secondary antibody for 30 minutes at room temperature.

Lane 1:

Rabbit IgG whole molecule at 0.05 µg

Lane 2:

Rabbit IgG F(ab) fragment at 0.05 µg

Lane 3:

Rabbit IgG F(c) fragment at 0.05 µg

Lane 4:

Rabbit IgM whole molecule at 0.05 µg

Lane 5:

Normal rabbit serum at 0.05 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

Lanes 1 - 5:

Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution

false

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)
  • WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)

false

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 565 Cy3®

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Guinea pig, Hamster, Cow, Human, Chicken

Pre-adsorbed

Yes

Conjugation

Cy2®

Excitation/Emission

Ex: 489nm, Em: 505nm

Applications

IHC-P, IHC-Fr, WB, ICC, ICC/IF, ELISA, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage buffer
pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Drug metabolism and disposition: the biological fate of chemicals 51:1230-1237 PubMed37349114

2023

NEAT1_2 and DAZAP1, Paraspeckle Components, Interact with PXR to Negatively Regulate CYP3A4 Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Rei Mitamura,Masataka Nakano,Motoki Isono,Kiamu Kurosawa,Tatsuki Fukami,Miki Nakajima

Theranostics 13:3550-3567 PubMed37441598

2023

Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Chen,Guihui Qin,Yuhong Liu,Moxin Li,Yue Li,Lun-Zhang Guo,Lidong Du,Weiming Zheng,Pei-Chun Wu,Yueh-Hsun Chuang,Xiaoyan Wang,Tzung-Dau Wang,Ja-An Annie Ho,Tzu-Ming Liu

Frontiers in microbiology 14:1142283 PubMed37125189

2023

Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Tianyuan Zhang,Luying Wang,Jingyue Ma,Xin Sun

Scientific reports 13:3253 PubMed36828892

2023

Experimental venous thrombus resolution is driven by IL-6 mediated monocyte actions.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea T Obi,Sriganesh B Sharma,Megan A Elfline,Catherine E Luke,Abigail R Dowling,Qing Cai,Andrew S Kimball,Mike Hollinstat,Livia Stanger,Bethany B Moore,Farouc A Jaffer,Peter K Henke

Journal of translational medicine 20:485 PubMed36274132

2022

Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruxia Deng,Xiansheng Lu,Chang Hong,Rui Cai,Ping Wang,Le Xiong,Xiaoyu Wang,Qiaoyu Chen,Jie Lin

Journal of healthcare engineering 2021:2771712 PubMed34956563

2021

CircRNA RERE Promotes the Oxidative Stress-Induced Apoptosis and Autophagy of Nucleus Pulposus Cells through the miR-299-5p/Galectin-3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Wang,Xingchao Zhou,Guorui Luo,Jin Zhang,Min Yang,Chao Song

Experimental and therapeutic medicine 21:67 PubMed33365067

2020

miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Ren,Ruanzhong Bao,Zhujun Guo,Jin Kai,Chen-Ge Cai,Zhu Li

Cell cycle (Georgetown, Tex.) 19:2530-2537 PubMed32892697

2020

Lidocaine alleviates cisplatin resistance and inhibits migration of MGC-803/DDP cells through decreasing miR-10b.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Zhang,Guangfeng Gu,Xuanfei Li,Chaopei Zhang

Cancer cell international 20:335 PubMed32714094

2020

LncRNA ANRIL/miR-7-5p/TCF4 axis contributes to the progression of T cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Li,Lan Gao,Jing Zhao,Dejun Liu,Hui Li,Min Hu

Cell communication and signaling : CCS 18:106 PubMed32641056

2020

Renal cancer-derived exosomes induce tumor immune tolerance by MDSCs-mediated antigen-specific immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Gao,Haoyu Xu,Nan Li,Hexi Wang,Lei Ma,Shiyou Chen,Jiayu Liu,Yongbo Zheng,Yao Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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