Goat Anti-Rabbit IgG H&L (Cy3 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry, ELISA and ICC.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 230 publications
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- Nature communications 15:4382024Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin ZhangPubMed 38200007
- Journal of applied genetics 65:181-1902023Long-run real-time PCR analysis of repetitive nuclear elements as a novel tool for DNA damage quantification in single cells: an approach validated on mouse oocytes and fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Kotarska,Łukasz Gąsior,Joanna Rudnicka,Zbigniew PolańskiPubMed 38110826
- Journal of innate immunity 15:876-8922023Advanced Glycation End Products-Induced Activation of Keratinocytes: A Mechanism Underlying Cutaneous Immune Response in Psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPan Kang,Jianru Chen,Shiyu Wang,Shaolong Zhang,Shuli Li,Sen Guo,Pu Song,Ling Liu,Gang Wang,Tianwen Gao,Weigang Zhang,Chunying LiPubMed 37989127
- Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association 131:656-6662023Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative Stress-Induced Pancreatic β-Cell Impairment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Liu,Yuanjun Zhang,Derong Shi,Cuihuan He,Guanghao XiaPubMed 37935388
- Cell death & disease 14:7072023Salicylate induces AMPK and inhibits c-MYC to activate a NRF2/ARE/miR-34a/b/c cascade resulting in suppression of colorectal cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunfeng Liu,Matjaz Rokavec,Zekai Huang,Heiko HermekingPubMed 37898661
- Royal Society open science 10:2310232023Mechanical loading due to muscle movement regulates establishment of the collagen network in the developing murine skeleton.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaima Ahmed,Andrew V Rogers,Niamh C NowlanPubMed 37859832
- Experimental and therapeutic medicine 26:5462023SPINT2 is involved in the proliferation, migration and phenotypic switching of aortic smooth muscle cells: Implications for the pathogenesis of thoracic aortic dissection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li,Changjun Yu,Kangmin Yu,Zhiyong Chen,Dan Xing,Binshan Zha,Wentao Xie,Huan OuyangPubMed 37928510
- Advanced healthcare materials 12:e23017722023Cu-Sr Bilayer Bioactive Glass Nanoparticles/Polydopamine Functionalized Polyetheretherketone Enhances Osteogenic Activity and Prevents Implant-Associated Infections through Spatiotemporal Immunomodulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuezhou Wu,Shicheng Huo,Shu Liu,Qimin Hong,You Wang,Zhuocheng LyuPubMed 37723927
- iScience 26:1079212023An early glycolysis burst in microglia regulates mitochondrial dysfunction in oligodendrocytes under neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHamid Suhail,Mohammad Nematullah,Faraz Rashid,Mir Sajad,Mena Fatma,Jaspreet Singh,Insha Zahoor,Wing Lee Cheung,Nivedita Tiwari,Kameshwar Ayasolla,Ashok Kumar,Nasrul Hoda,Ramandeep Rattan,Shailendra GiriPubMed 37841597
- Journal of natural medicines 78:53-672023Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jun Li,Ting Liu,Yuan WangPubMed 37668824
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human, Mouse, Rat, Sheep
- Conjugation
- Cy3®
- Applications
- ICC, IHC-Fr, Flow Cyt, IHC-P, ELISA, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/100 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Goat Anti-Rabbit IgG H&L (Cy3 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry, ELISA and ICC.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 230 publications
Key facts
- Description
- Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human, Mouse, Rat, Sheep
- Conjugation
- Cy3®
- Applications
- ICC, IHC-Fr, Flow Cyt, IHC-P, ELISA, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Purification technique
- Affinity purification Immunogen
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody reacts with the heavy and light chains of Rabbit IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.
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5 product images
Bockor et al PLoS One. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954. eCollection 2014. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter "n" marks the nucleoli. Scale bar is 5 μm.
Islam et al PLoS One. 2015 Jun 8;10(6):e0128306. doi: 10.1371/journal.pone.0128306. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).
Photomicrographs are representative of four independent samples.
Cell nuclei were counterstained with DAPI (blue).
Original magnification: 200x.
Buchholz et al PLoS One. 2015 Apr 7;10(4):e0122946. doi: 10.1371/journal.pone.0122946. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Examples of the different antibody stainings for functional analyses.Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).
Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).
Original magnifications are indicated in the images.
Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Lane M: 3 μL Molecular ladder.Lane 1: Rabbit IgG whole molecule (p/n 011-0102).
Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).
Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).
Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).
Lane 5: Normal Rabbit Serum (p/n B309).
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: ab6939 used at 1/1000 for 60 min at RT.
Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Lane 1: Rabbit IgG.Load: 50 ng per lane.
Secondary antibody: ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
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