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AB6939

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

5

(3 Reviews)

|

(236 Publications)

Goat Anti-Rabbit IgG H&L (Cy3 ®) is a preadsorbed secondary antibody that is suitable for IHC, ICC/IF, Flow Cytometry, ELISA and ICC.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 230 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

5 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).

Photomicrographs are representative of four independent samples.

Cell nuclei were counterstained with DAPI (blue).

Original magnification : 200x.

Islam et al PLoS One. 2015 Jun 8;10(6):e0128306. doi: 10.1371/journal.pone.0128306. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Examples of the different antibody stainings for functional analyses.

Left Panel : Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).

Right Panel : Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).

Original magnifications are indicated in the images.

Buchholz et al PLoS One. 2015 Apr 7;10(4):e0122946. doi: 10.1371/journal.pone.0122946. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.

IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter "n" marks the nucleoli. Scale bar is 5 μm.

Bockor et al PLoS One. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954. eCollection 2014. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Lane 1 : Rabbit IgG.

Load : 50 ng per lane.

Secondary antibody : ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.

Predicted/Observed size : 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

false

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)
  • WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Lane M : 3 μL Molecular ladder.

Lane 1 : Rabbit IgG whole molecule (p/n 011-0102).

Lane 2 : Rabbit IgG F(ab) Fragment (p/n 011-0105).

Lane 3 : Rabbit IgG F(c) Fragment (p/n 010-0103).

Lane 4 : Rabbit IgM Whole Molecule (p/n 011-0107).

Lane 5 : Normal Rabbit Serum (p/n B309).

All samples were reduced. Load : 50 ng per lane. Blocked for 30 min at RT.

Primary Antibody : ab6939 used at 1/1000 for 60 min at RT.

Secondary antibody : Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.

Predicted/Obsevered Size : 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.

Rabbit F(c) migrates slightly higher.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

false

  • Beta-galactosidase

    Goat Anti-Rabbit IgG H&L (beta-galactosidase)

  • Glucose Oxidase

    Goat Anti-Rabbit IgG H&L (Glucose Oxidase)

  • HRP polymer

    Goat Anti-Rabbit IgG H&L (HRP polymer)

  • Unconjugated

    Goat Anti-Rabbit IgG H&L preadsorbed

  • Alkaline Phosphatase

    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)

  • 667 Cy5®

    Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed

  • 505 Cy2®

    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

  • 519 FITC

    Goat Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat Anti-Rabbit IgG H&L (TRITC)

  • 6nm Gold

    Goat Anti-Rabbit IgG H&L (6nm Gold)

  • 618 DyLight® 594

    Goat Anti-Rabbit IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Rabbit IgG H&L (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680)

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

  • 519 Alexa Fluor® 488

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)

  • 665 Alexa Fluor® 647

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

  • 702 Alexa Fluor® 680

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • 775 Alexa Fluor® 750

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405)

  • 578 PE

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed

  • 615 Texas Red®

    Goat Anti-Rabbit IgG H&L (Texas Red ®)

  • 660 APC

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed

  • 518 DyLight® 488

    Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

  • 703 Cy5.5®

    Goat Anti-Rabbit IgG H&L (Cy5.5 ®) preadsorbed

  • 596 Cy3.5®

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed

  • 805 Alexa Fluor® 790

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human, Mouse, Rat, Sheep,

Pre-adsorbed

Yes

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Applications

ICC, ICC/IF, ELISA, Flow Cyt, IHC-Fr, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }
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You may be interested in:

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

5

20 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This antibody reacts with the heavy and light chains of Rabbit IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (236)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang

Journal of applied genetics 65:181-190 PubMed38110826

2023

Long-run real-time PCR analysis of repetitive nuclear elements as a novel tool for DNA damage quantification in single cells: an approach validated on mouse oocytes and fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Kotarska,Łukasz Gąsior,Joanna Rudnicka,Zbigniew Polański

Journal of innate immunity 15:876-892 PubMed37989127

2023

Advanced Glycation End Products-Induced Activation of Keratinocytes: A Mechanism Underlying Cutaneous Immune Response in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Kang,Jianru Chen,Shiyu Wang,Shaolong Zhang,Shuli Li,Sen Guo,Pu Song,Ling Liu,Gang Wang,Tianwen Gao,Weigang Zhang,Chunying Li

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association 131:656-666 PubMed37935388

2023

Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative Stress-Induced Pancreatic β-Cell Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Yuanjun Zhang,Derong Shi,Cuihuan He,Guanghao Xia

Cell death & disease 14:707 PubMed37898661

2023

Salicylate induces AMPK and inhibits c-MYC to activate a NRF2/ARE/miR-34a/b/c cascade resulting in suppression of colorectal cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfeng Liu,Matjaz Rokavec,Zekai Huang,Heiko Hermeking

Royal Society open science 10:231023 PubMed37859832

2023

Mechanical loading due to muscle movement regulates establishment of the collagen network in the developing murine skeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Saima Ahmed,Andrew V Rogers,Niamh C Nowlan

Experimental and therapeutic medicine 26:546 PubMed37928510

2023

SPINT2 is involved in the proliferation, migration and phenotypic switching of aortic smooth muscle cells: Implications for the pathogenesis of thoracic aortic dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Changjun Yu,Kangmin Yu,Zhiyong Chen,Dan Xing,Binshan Zha,Wentao Xie,Huan Ouyang

Advanced healthcare materials 12:e2301772 PubMed37723927

2023

Cu-Sr Bilayer Bioactive Glass Nanoparticles/Polydopamine Functionalized Polyetheretherketone Enhances Osteogenic Activity and Prevents Implant-Associated Infections through Spatiotemporal Immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuezhou Wu,Shicheng Huo,Shu Liu,Qimin Hong,You Wang,Zhuocheng Lyu

iScience 26:107921 PubMed37841597

2023

An early glycolysis burst in microglia regulates mitochondrial dysfunction in oligodendrocytes under neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Hamid Suhail,Mohammad Nematullah,Faraz Rashid,Mir Sajad,Mena Fatma,Jaspreet Singh,Insha Zahoor,Wing Lee Cheung,Nivedita Tiwari,Kameshwar Ayasolla,Ashok Kumar,Nasrul Hoda,Ramandeep Rattan,Shailendra Giri

Journal of natural medicines 78:53-67 PubMed37668824

2023

Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jun Li,Ting Liu,Yuan Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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