Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).

Photomicrographs are representative of four independent samples.

Cell nuclei were counterstained with DAPI (blue).

Original magnification : 200x.

Islam et al PLoS One. 2015 Jun 8;10(6):e0128306. doi: 10.1371/journal.pone.0128306. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Examples of the different antibody stainings for functional analyses.

Left Panel : Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).

Right Panel : Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).

Original magnifications are indicated in the images.

Buchholz et al PLoS One. 2015 Apr 7;10(4):e0122946. doi: 10.1371/journal.pone.0122946. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.

IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter "n" marks the nucleoli. Scale bar is 5 μm.

Bockor et al PLoS One. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954. eCollection 2014. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Lane 1 : Rabbit IgG.

Load : 50 ng per lane.

Secondary antibody : ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.

Predicted/Observed size : 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

false

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (AB6939)

Lane M : 3 μL Molecular ladder.

Lane 1 : Rabbit IgG whole molecule (p/n 011-0102).

Lane 2 : Rabbit IgG F(ab) Fragment (p/n 011-0105).

Lane 3 : Rabbit IgG F(c) Fragment (p/n 010-0103).

Lane 4 : Rabbit IgM Whole Molecule (p/n 011-0107).

Lane 5 : Normal Rabbit Serum (p/n B309).

All samples were reduced. Load : 50 ng per lane. Blocked for 30 min at RT.

Primary Antibody : ab6939 used at 1/1000 for 60 min at RT.

Secondary antibody : Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.

Predicted/Obsevered Size : 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.

Rabbit F(c) migrates slightly higher.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

false